BMRBj - BMREntryMaster

Found 1 Document (0.833 seconds)

BMRB: 34534

6ZTG

Spor protein DedD

Authors

Pazos, M., Peters, K., Boes, A., Safaei, Y., Kenward, C., Caveney, N.A., Laguri, C., Breukink, E., Strynadka, N.C.J., Simorre, J.P., Terrak, M., Vollmer, W.

Assembly

Cell division protein DedD

Entity

1. Cell division protein DedD (polymer, Thiol state: not present), 193 monomers, 20133.69 Da Detail

DGQKKHYQDE FAAIPLVPKA GDRDEPDMMP AATQALPTQP PEGAAEEVRA GDAAAPSLDP ATIAANNTEF EPEPAPVAPP KPKPVEPPKP KVEAPPAPKP EPKPVVEEKA APTGKAYVVQ LGALKNADKV NEIVGKLRGA GYRVYTSPST PVQGKITRIL VGPDASKDKL KGSLGELKQL SGLSGVVMGY TPN

Formula weight

20133.69 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.2 %, Completeness: 65.3 %, Completeness (bb): 76.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	65.3 % (1408 of 2157)	64.3 % (730 of 1135)	62.1 % (523 of 842)	86.1 % (155 of 180)
Backbone	76.0 % (835 of 1098)	86.6 % (322 of 372)	63.6 % (358 of 563)	95.1 % (155 of 163)
Sidechain	59.0 % (729 of 1236)	53.5 % (408 of 763)	70.4 % (321 of 456)	0.0 % (0 of 17)
Aromatic	32.8 % (21 of 64)	34.4 % (11 of 32)	31.3 % (10 of 32)
Methyl	74.3 % (150 of 202)	72.3 % (73 of 101)	76.2 % (77 of 101)

1. entity 1

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 Pa, Temperature 298 K, pH 7, Details 1.08 mM [U-13C; U-15N] DedD, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DedD	[U-13C; U-15N]		1.08 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6ZTG, Strand ID: A Detail

Release date

2020-08-10

Citation

SPOR Proteins Are Required for Functionality of Class A Penicillin-Binding Proteins in Escherichia coli
Pazos, M., Peters, K., Boes, A., Safaei, Y., Kenward, C., Caveney, N.A., Laguri, C., Breukink, E., Strynadka, N., Simorre, J.P., Terrak, M., Vollmer, W.
mBio (2020), 11, e02796-20-e02796-20, PubMed 33144379 , DOI 10.1128/mBio.02796-20 , Abstract

Related entities 1. Cell division protein DedD, : 1 : 2 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34534_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34534_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1463		88.1 (chain: 1, length: 193)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 193, Sequence coverage: 88.1%
- Total number of assignments
  - ¹H chemical shifts: 836
  - ¹³C chemical shifts: 477
  - ¹⁵N chemical shifts: 150
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1135	696	61.3
¹³C chemical shifts	842	477	56.7
¹⁵N chemical shifts	180	150	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	372	314	84.4
¹³C chemical shifts	386	169	43.8
¹⁵N chemical shifts	163	150	92.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	763	382	50.1
¹³C chemical shifts	456	308	67.5
¹⁵N chemical shifts	17	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	104	70	67.3
¹³C chemical shifts	104	76	73.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	32	10	31.2
¹³C chemical shifts	32	9	28.1

Keywords CELL CYCLE, bacteria, peptidoglycan synthesis, preseptal

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Found 1 Document (0.833 seconds)

BMRB: 34534

Sample

Related entities 1. Cell division protein DedD, : 1 : 2 entities Detail

Experiments performed 3 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail