BMRBj - BMREntryMaster

Found 1 Document (0.465 seconds)

BMRB: 34556

7AAO

Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2

Authors

Kawale, A.A., Kang, H.S., Sattler, M.

Assembly

U2 snRNP auxiliary factor large subunit

Entity

1. U2 snRNP auxiliary factor large subunit (polymer), 122 monomers, 13174.97 Da Detail

SISVSAMESY RSFRVPAINV AQQPAVTLPV TTIVPDSPNK IYVGGLPTCL NQDQVKELLQ SFGELKGLNL VMDTNTNLNK GFAFFEYCDP SVTDHAIAGL HGMLLGDRRL VVQRSIPGGK NA

Formula weight

13174.97 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.9 %, Completeness (bb): 84.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.9 % (1126 of 1391)	85.3 % (611 of 716)	73.4 % (398 of 542)	88.0 % (117 of 133)
Backbone	84.4 % (604 of 716)	99.6 % (245 of 246)	68.8 % (245 of 356)	100.0 % (114 of 114)
Sidechain	80.4 % (633 of 787)	77.9 % (366 of 470)	88.6 % (264 of 298)	15.8 % (3 of 19)
Aromatic	75.6 % (62 of 82)	78.0 % (32 of 41)	73.2 % (30 of 41)
Methyl	80.5 % (124 of 154)	77.9 % (60 of 77)	83.1 % (64 of 77)

1. entity 1

SISVSAMESY RSFRVPAINV AQQPAVTLPV TTIVPDSPNK IYVGGLPTCL NQDQVKELLQ SFGELKGLNL VMDTNTNLNK GFAFFEYCDP SVTDHAIAGL HGMLLGDRRL VVQRSIPGGK NA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM [U-13C; U-15N] LS2 Linker RRM2, 0.02 mM [U-13C; U-15N] potassium phosphate, 0.05 mM [U-13C; U-15N] sodium chloride, 0.002 mM [U-13C; U-15N] DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

Release date

2020-10-18

Citation

Structural evolution of the tissue-specific U2AF2 paralog and alternative splicing factor LS2
Kawale, A.A., Kang, H.S., Sattler, M.

Related entities 1. U2 snRNP auxiliary factor large subunit, : 1 : 1 : 34 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.0 mM [U-13C; U-15N] LS2 Linker RRM2, 0.02 mM [U-13C; U-15N] potassium phosphate, 0.05 mM [U-13C; U-15N] sodium chloride, 0.002 mM [U-13C; U-15N] DTT, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

3 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

4 3D 1H-15N NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

5 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

6 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

7 3D HNCA

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

8 3D HNCACB

Instrument

Bruker AVANCE - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

9 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE - 500 MHz

Sample

#	Name	Isotope labeling	Concentration
1	LS2 Linker RRM2	[U-13C; U-15N]	1.0 mM
2	potassium phosphate	natural abundance	0.02 mM
3	sodium chloride	natural abundance	0.05 mM
4	DTT	natural abundance	0.002 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34556_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34556_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1213		100.0 (chain: 1, length: 122)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 122, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 716
  - ¹³C chemical shifts: 381
  - ¹⁵N chemical shifts: 116
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	716	598	83.5
¹³C chemical shifts	542	381	70.3
¹⁵N chemical shifts	133	116	87.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	246	245	99.6
¹³C chemical shifts	244	122	50.0
¹⁵N chemical shifts	114	114	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	470	353	75.1
¹³C chemical shifts	298	259	86.9
¹⁵N chemical shifts	19	2	10.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	80	59	73.8
¹³C chemical shifts	80	64	80.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	41	27	65.9
¹³C chemical shifts	41	27	65.9

Keywords G quadruplex, LS2, RNA BINDING PROTEIN, RNA-protein interactions, RRM RNA binding domain, U2AF, alternative splicing, structural biology

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Found 1 Document (0.465 seconds)

BMRB: 34556

Sample

Related entities 1. U2 snRNP auxiliary factor large subunit, : 1 : 1 : 34 entities Detail

Experiments performed 9 experiments Detail

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Chemical shift validation 3 contents Detail