Solution structure of the fluorogen-activating protein FAST in the apo state
MEHVAFGSED IENTLAKMDD GQLDGLAFGA IQLDGDGNIL QYNAAEGDIT GRDPKQVIGK NFFKDVAPGT DSPEFYGKFK EGVASGNLNT MFEWMIPTSR GPTKVKVHMK KALSGDSYWV FVKRVKLAAA LEHHHHHH
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 91.2 % (1449 of 1588) | 93.8 % (769 of 820) | 89.7 % (556 of 620) | 83.8 % (124 of 148) |
Backbone | 90.6 % (741 of 818) | 90.6 % (259 of 286) | 92.7 % (370 of 399) | 84.2 % (112 of 133) |
Sidechain | 92.7 % (828 of 893) | 95.5 % (510 of 534) | 89.0 % (306 of 344) | 80.0 % (12 of 15) |
Aromatic | 71.2 % (114 of 160) | 85.0 % (68 of 80) | 56.4 % (44 of 78) | 100.0 % (2 of 2) |
Methyl | 100.0 % (132 of 132) | 100.0 % (66 of 66) | 100.0 % (66 of 66) |
1. entity 1
MEHVAFGSED IENTLAKMDD GQLDGLAFGA IQLDGDGNIL QYNAAEGDIT GRDPKQVIGK NFFKDVAPGT DSPEFYGKFK EGVASGNLNT MFEWMIPTSR GPTKVKVHMK KALSGDSYWV FVKRVKLAAA LEHHHHHHSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 318 K, pH 7.0 (±0.02), Details 1 mM [U-13C; U-15N] FAST, 20 mM sodium phosphate, 20 mM sodium chloride, 0.1 % sodium azide, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | FAST | [U-13C; U-15N] | 1 (±0.1) mM | |
2 | sodium phosphate | natural abundance | 20 (±0.1) mM | |
3 | sodium chloride | natural abundance | 20 (±0.1) mM | |
4 | sodium azide | natural abundance | 0.1 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 318 K, pH 7.0 (±0.02), Details 1 mM [U-13C; U-15N] FAST, 20 mM sodium phosphate, 20 mM sodium chloride, 0.1 % sodium azide, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | FAST | [U-13C; U-15N] | 1 (±0.1) mM | |
6 | sodium phosphate | natural abundance | 20 (±0.1) mM | |
7 | sodium chloride | natural abundance | 20 (±0.1) mM | |
8 | sodium azide | natural abundance | 0.1 % |