BMRB: 34591

GA attached to an i-motif clip at 3'-end

Authors

Novotny, A., Novotny, J.

Assembly

(CH+)C(CH+)GAGA, C(CH+)CGAGA

Entity

1. (CH+)C(CH+)GAGA, C(CH+)CGAGA, entity 1 (polymer, Thiol state: not present), 5 monomers, 1221.784 × 2 Da Detail

GAXCX

2. (CH+)C(CH+)GAGA, C(CH+)CGAGA, entity 2 (polymer, Thiol state: not present), 5 monomers, 1543.008 × 2 Da Detail

GACXX

Total weight

5529.584 Da

Max. entity weight

1543.008 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 30.0 %, Completeness: 34.5 %, Completeness (bb): 28.6 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	34.5 % (20 of 58)	34.5 % (20 of 58)
Suger, PO4	28.6 % (12 of 42)	28.6 % (12 of 42)
Nucleobase	50.0 % (8 of 16)	50.0 % (8 of 16)
Aromatic	50.0 % (6 of 12)	50.0 % (6 of 12)

1. entity 1

GAXCX

2. entity 2

GACX

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5 (±0.2), Details 0.8 mM GAGACC(5MeC), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	GAGACC(5MeC), entity_1	natural abundance		0.8 (±0.1) mM
2	GAGACC(5MeC), entity_2	natural abundance		0.8 (±0.1) mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 278 K, pH 5 (±0.2), Details 0.8 mM GAGACC(5MeC), 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	GAGACC(5MeC), entity_1	natural abundance		0.8 (±0.1) mM
4	GAGACC(5MeC), entity_2	natural abundance		0.8 (±0.1) mM

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Release date

2021-03-18

Citation

Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip
Novotny, A., Novotny, J., Kejnovka, I., Vorlickova, M., Fiala, R., Marek, R.
Nucleic Acids Res. (2021), 49, 11425-11437, PubMed 34718718 , DOI 10.1093/nar/gkab915 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail