BMRB: 34592

AG repetition attached to a compact i-motif clip at 3'-end

Authors

Novotny, A., Novotny, J.

Assembly

(CH+)C(CH+)GAGA, C(CH+)CGAGA

Entity

1. (CH+)C(CH+)GAGA, C(CH+)CGAGA, entity 1 (polymer, Thiol state: not present), 7 monomers, 2172.401 × 2 Da Detail

AGAGXCX

2. (CH+)C(CH+)GAGA, C(CH+)CGAGA, entity 2 (polymer, Thiol state: not present), 7 monomers, 2171.393 × 2 Da Detail

AGAGCXC

Total weight

8687.588 Da

Max. entity weight

2172.401 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 35.7 %, Completeness: 28.6 %, Completeness (bb): 23.4 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	28.6 % (30 of 105)	28.6 % (30 of 105)
Suger, PO4	23.4 % (18 of 77)	23.4 % (18 of 77)
Nucleobase	42.9 % (12 of 28)	42.9 % (12 of 28)
Aromatic	45.5 % (10 of 22)	45.5 % (10 of 22)

1. entity 1

AGAGXCX

2. entity 2

AGAGCXC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5 (±0.2), Details 1.3 mM AGAGCCC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	AGAGCCC, entity_1	natural abundance		1.3 (±0.1) mM
2	AGAGCCC, entity_2	natural abundance		1.3 (±0.1) mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 278 K, pH 5 (±0.2), Details 1.3 mM AGAGCCC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	AGAGCCC, entity_1	natural abundance		1.3 (±0.1) mM
4	AGAGCCC, entity_2	natural abundance		1.3 (±0.1) mM

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Release date

2021-03-18

Citation

Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip
Novotny, A., Novotny, J., Kejnovka, I., Vorlickova, M., Fiala, R., Marek, R.
Nucleic Acids Res. (2021), 49, 11425-11437, PubMed 34718718 , DOI 10.1093/nar/gkab915 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Experiments performed 2 experiments Detail

Chemical shift validation 4 contents Detail