BMRB: 34593

AG repetition attached to an extended i-motif clip at 3'-end

Authors

Novotny, A., Novotny, J.

Assembly

(CH+)C(CH+)GAGA, C(CH+)CGAGA

Entity

1. (CH+)C(CH+)GAGA, C(CH+)CGAGA, entity 1 (polymer, Thiol state: not present), 7 monomers, 1864.196 × 2 Da Detail

AGAGXCX

2. (CH+)C(CH+)GAGA, C(CH+)CGAGA, entity 2 (polymer, Thiol state: not present), 7 monomers, 2185.420 × 2 Da Detail

AGAGCXX

Total weight

8099.232 Da

Max. entity weight

2185.42 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 35.7 %, Completeness: 31.9 %, Completeness (bb): 25.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polydeoxyribonucleotide

	Total	1H
All	31.9 % (30 of 94)	31.9 % (30 of 94)
Suger, PO4	25.7 % (18 of 70)	25.7 % (18 of 70)
Nucleobase	50.0 % (12 of 24)	50.0 % (12 of 24)
Aromatic	50.0 % (10 of 20)	50.0 % (10 of 20)

1. entity 1

AGAGXCX

2. entity 2

AGAGCX

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 278 K, pH 5 (±0.2), Details 0.8 mM AGAGCCmC, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	AGAGCCmC, entity_1	natural abundance		0.8 (±0.1) mM
2	AGAGCCmC, entity_2	natural abundance		0.8 (±0.1) mM

Hide sample codition

Release date

2021-03-18

Citation

Revealing structural peculiarities of homopurine GA repetition stuck by i-motif clip
Novotny, A., Novotny, J., Kejnovka, I., Vorlickova, M., Fiala, R., Marek, R.
Nucleic Acids Res. (2021), 49, 11425-11437, PubMed 34718718 , DOI 10.1093/nar/gkab915 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail