NMR structure of GIPC1-GH2 domain
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 79.9 % (748 of 936) | 92.1 % (443 of 481) | 61.2 % (226 of 369) | 91.9 % (79 of 86) |
Backbone | 93.7 % (461 of 492) | 94.2 % (161 of 171) | 92.5 % (223 of 241) | 96.2 % (77 of 80) |
Sidechain | 68.8 % (357 of 519) | 91.0 % (282 of 310) | 36.0 % (73 of 203) | 33.3 % (2 of 6) |
Aromatic | 35.9 % (28 of 78) | 71.8 % (28 of 39) | 0.0 % (0 of 38) | 0.0 % (0 of 1) |
Methyl | 59.1 % (52 of 88) | 97.7 % (43 of 44) | 20.5 % (9 of 44) |
1. entity 1
GAMGDLPSAF EEKAIEKVDD LLESYMGIRD TELAATMVEL GKDKRNPDEL AEALDERLGD FAFPDEFVFD VWGAIGDAKV GRYSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1.0 mM [U-15N] GIPC1-GH2, 20 mM TRIS, 0.1 mM EDTA, 0.1 mM PMSF, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | GIPC1-GH2 | [U-15N] | 1.0 mM | |
2 | TRIS | natural abundance | 20 mM | |
3 | EDTA | natural abundance | 0.1 mM | |
4 | PMSF | natural abundance | 0.1 mM |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1.0 mM [U-13C; U-15N] GIPC1-GH2, 20 mM TRIS, 0.1 mM EDTA, 0.1 mM PMSF, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
5 | GIPC1-GH2 | [U-13C; U-15N] | 1.0 mM | |
6 | TRIS | natural abundance | 20 mM | |
7 | EDTA | natural abundance | 0.1 mM | |
8 | PMSF | natural abundance | 0.1 mM |
Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.2, Details 1.0 mM GIPC1-GH2, 20 mM TRIS, 0.1 mM EDTA, 0.1 mM PMSF, 100% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
9 | GIPC1-GH2 | natural abundance | 1.0 mM | |
10 | TRIS | natural abundance | 20 mM | |
11 | EDTA | natural abundance | 0.1 mM | |
12 | PMSF | natural abundance | 0.1 mM |