BMRBj - BMREntryMaster

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BMRB: 34629

2R8U

EB1 bound to MACF peptide

Authors

Almeida, T.B., Barsukov, I.L.

Assembly

Microtubule-associated protein RP/EB family member 1, 11MACF

Entity

1. Microtubule-associated protein RP/EB family member 1 (polymer), 70 monomers, 8042.918 × 2 Da Detail

DEAAELMQQV KVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG

2. 11MACF (polymer, Thiol state: not present), 11 monomers, 1279.528 × 2 Da Detail

KPSKIPTPQR K

Total weight

18644.893 Da

Max. entity weight

8042.918 Da

Source organism

Mus musculus , Homo sapiens

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 80.1 %, Completeness (bb): 77.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.1 % (783 of 978)	86.4 % (445 of 515)	69.6 % (261 of 375)	87.5 % (77 of 88)
Backbone	77.1 % (367 of 476)	93.2 % (151 of 162)	61.3 % (146 of 238)	92.1 % (70 of 76)
Sidechain	83.0 % (480 of 578)	83.3 % (294 of 353)	84.0 % (179 of 213)	58.3 % (7 of 12)
Aromatic	100.0 % (46 of 46)	100.0 % (23 of 23)	100.0 % (23 of 23)
Methyl	97.8 % (90 of 92)	97.8 % (45 of 46)	97.8 % (45 of 46)

1. Microtubule-associated protein RP/EB family member 1

DEAAELMQQV KVLKLTVEDL EKERDFYFGK LRNIELICQE NEGENDPVLQ RIVDILYATD EGFVIPDEGG

2. 11MACF

KPSKIPTPQR K

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

Release date

2021-06-14

Citation

EB1 bound to MACF peptide
Almeida, T.B., Barsukov, I.L.

Related entities 1. Microtubule-associated protein RP/EB family member 1, : 1 : 5 : 10 : 38 entities Detail

Related entities 2. 11MACF, : 3 : 1 entities Detail

Interaction partners 2. 11MACF, : 47 interactors Detail

More details for 47 interactors identified by 20 citations

Experiments performed 9 experiments Detail

1 2D 1H-13C HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

2 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

3 2D 1H-1H NOESY

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

4 2D 1H-15N HSQC

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

5 3D CBCA(CO)NH

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

6 3D 1H-15N NOESY

Instrument

Bruker AVANCE II - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

7 3D HCCH-TOCSY

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

8 3D 1H-13C NOESY

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

9 3D HBHA(CO)NH

Instrument

Bruker AVANCE II - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.15 mM [U-13C; U-15N] cEB1, 1.265 mM 11MACF, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	cEB1	[U-13C; U-15N]		1.15 mM
2	11MACF	natural abundance		1.265 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34629_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34629_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	849		90.9 (chain: 2, length: 11), 100.0 (chain: 1, length: 70)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 2, Chain length: 11, Sequence coverage: 90.9%
  - Entity_assembly_ID: 1, Chain length: 70, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 538
  - ¹³C chemical shifts: 239
  - ¹⁵N chemical shifts: 72
- Completeness of assignments
  - Entity_assemble_ID: 2
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 2
  - Chain_ID: 1

Keywords +TIPs, Leucine zipper, PROTEIN BINDING, homodimer, microtubule