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Found 1 Document (1.895 seconds)

BMRB: 34638

7OVC

Structure of the human UFC1 protein in complex with the UBA5 C-terminal UFC1-binding motif.

Authors

Wesch, W., Loehr, F., Rogova, N., Doetsch, V., Rogov, V.V.

Assembly

Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5

Entity

1. Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5, entity 1 (polymer), 167 monomers, 19458.14 Da Detail

MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ

2. Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5, entity 2 (polymer, Thiol state: not present), 27 monomers, 3032.441 Da Detail

GMSVTELTVE DSGESLEDLM AKMKNMW

Total weight

22490.582 Da

Max. entity weight

19458.14 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 86.9 %, Completeness (bb): 80.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.9 % (2049 of 2357)	95.4 % (1172 of 1228)	74.3 % (679 of 914)	92.1 % (198 of 215)
Backbone	80.9 % (925 of 1144)	95.9 % (374 of 390)	65.6 % (374 of 570)	96.2 % (177 of 184)
Sidechain	93.3 % (1302 of 1395)	95.2 % (798 of 838)	91.8 % (483 of 526)	67.7 % (21 of 31)
Aromatic	81.3 % (169 of 208)	91.3 % (95 of 104)	69.1 % (67 of 97)	100.0 % (7 of 7)
Methyl	98.1 % (204 of 208)	98.1 % (102 of 104)	98.1 % (102 of 104)

1. entity 1

MADEATRRVV SEIPVLKTNA GPRDRELWVQ RLKEEYQSLI RYVENNKNAD NDWFRLESNK EGTRWFGKCW YIHDLLKYEF DIEFDIPITY PTTAPEIAVP ELDGKTAKMY RGGKICLTDH FKPLWARNVP KFGLAHLMAL GLGPWLAVEI PDLIQKGVIQ HKEKCNQ

2. entity 2

GMSVTELTVE DSGESLEDLM AKMKNMW

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.2) K, pH 7.5 (±0.05), Details 1.0 mM [U-100% 13C; U-100% 15N] Ubiquitin-fold modifier-conjugating enzyme 1, 1.0 mM Ubiquitin-like modifier-activating enzyme 5, 50 mM TRIS, 100 mM sodium chloride, 2 mM TCEP, 5 mM AEBSF protease inhibitor, 0.15 mM DSS, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.2) K, pH 7.5 (±0.05), Details 1.2 mM Ubiquitin-fold modifier-conjugating enzyme 1, 0.3 mM [U-100% 13C; U-100% 15N] Ubiquitin-like modifier-activating enzyme 5, 50 mM TRIS, 100 mM sodium chloride, 2 mM TCEP, 5 mM AEBSF protease inhibitor, 0.15 mM DSS, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
8	Ubiquitin-fold modifier-conjugating enzyme 1	natural abundance	1.2 (±0.05) mM
9	Ubiquitin-like modifier-activating enzyme 5	[U-100% 13C; U-100% 15N]	0.3 (±0.05) mM
10	TRIS	natural abundance	50 (±1.0) mM
11	sodium chloride	natural abundance	100 (±1.0) mM
12	TCEP	natural abundance	2 (±0.1) mM
13	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
14	DSS	natural abundance	0.15 (±0.01) mM

Release date

2021-07-11

Citation

A Concerted Action of UBA5 C-Terminal Unstructured Regions Is Important for Transfer of Activated UFM1 to UFC1
Wesch, W., Loehr, F., Rogova, N., Doetsch, V., Rogov, V.V.
Int. J. Mol. Sci. (2021), 22, 7390-7390, PubMed 34299007 , DOI 10.3390/ijms22147390 , Abstract

Related entities 1. Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5, entity 1, : 1 : 13 entities Detail

Related entities 2. Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5, entity 2, : 1 : 3 : 9 entities Detail

Interaction partners 2. Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5, entity 2, : 19 interactors Detail

More details for 19 interactors identified by 6 citations

Experiments performed 16 experiments Detail

1 3D [15N,1H]-TROSY-(H)C(CC)(CO)NH-TOCSY

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.2) K, pH 7.5 (±0.05), Details 1.0 mM [U-100% 13C; U-100% 15N] Ubiquitin-fold modifier-conjugating enzyme 1, 1.0 mM Ubiquitin-like modifier-activating enzyme 5, 50 mM TRIS, 100 mM sodium chloride, 2 mM TCEP, 5 mM AEBSF protease inhibitor, 0.15 mM DSS, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

2 3D [15N,1H]-TROSY-H(C)(CC)(CO)NH-TOCSY

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

3 3D BEST-[15N,1H]-TROSY-HNCACB

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

4 3D F1-13C/15N-filtered NOESY-[13C,1H]-HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

5 3D F1-13C/15N-filtered NOESY-[15N,1H]-SOFAST-HMQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

6 3D F1-13C/15N-filtered NOESY-[13C,1H]-SOFAST-HMQC (aro)

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

7 3D NOESY-[13C,1H]-SOFAST-HMQC (aro)

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

8 3D NOESY-[13C,1H]-HSQC (aliphatic region)

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

9 3D NOESY-BEST-[15N,1H]-TROSY

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
1	Ubiquitin-fold modifier-conjugating enzyme 1	[U-100% 13C; U-100% 15N]	1.0 (±0.05) mM
2	Ubiquitin-like modifier-activating enzyme 5	natural abundance	1.0 (±0.05) mM
3	TRIS	natural abundance	50 (±1.0) mM
4	sodium chloride	natural abundance	100 (±1.0) mM
5	TCEP	natural abundance	2 (±0.1) mM
6	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
7	DSS	natural abundance	0.15 (±0.01) mM

10 3D BEST-[15N,1H]-TROSY-HNCA

Instrument

Bruker AVANCE III - 950 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.2) K, pH 7.5 (±0.05), Details 1.2 mM Ubiquitin-fold modifier-conjugating enzyme 1, 0.3 mM [U-100% 13C; U-100% 15N] Ubiquitin-like modifier-activating enzyme 5, 50 mM TRIS, 100 mM sodium chloride, 2 mM TCEP, 5 mM AEBSF protease inhibitor, 0.15 mM DSS, 95% H2O/5% D2O.

#	Name	Isotope labeling	Concentration
8	Ubiquitin-fold modifier-conjugating enzyme 1	natural abundance	1.2 (±0.05) mM
9	Ubiquitin-like modifier-activating enzyme 5	[U-100% 13C; U-100% 15N]	0.3 (±0.05) mM
10	TRIS	natural abundance	50 (±1.0) mM
11	sodium chloride	natural abundance	100 (±1.0) mM
12	TCEP	natural abundance	2 (±0.1) mM
13	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
14	DSS	natural abundance	0.15 (±0.01) mM

11 3D BEST-[15N,1H]-TROSY-HNCACB

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
8	Ubiquitin-fold modifier-conjugating enzyme 1	natural abundance	1.2 (±0.05) mM
9	Ubiquitin-like modifier-activating enzyme 5	[U-100% 13C; U-100% 15N]	0.3 (±0.05) mM
10	TRIS	natural abundance	50 (±1.0) mM
11	sodium chloride	natural abundance	100 (±1.0) mM
12	TCEP	natural abundance	2 (±0.1) mM
13	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
14	DSS	natural abundance	0.15 (±0.01) mM

12 3D F1-13C/15N-filtered NOESY-[13C,1H]-HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Concentration
8	Ubiquitin-fold modifier-conjugating enzyme 1	natural abundance	1.2 (±0.05) mM
9	Ubiquitin-like modifier-activating enzyme 5	[U-100% 13C; U-100% 15N]	0.3 (±0.05) mM
10	TRIS	natural abundance	50 (±1.0) mM
11	sodium chloride	natural abundance	100 (±1.0) mM
12	TCEP	natural abundance	2 (±0.1) mM
13	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
14	DSS	natural abundance	0.15 (±0.01) mM

13 3D [15N,1H]-TROSY-(H)C(CC)(CO)NH-TOCSY

Instrument

Bruker AVANCE III HD - 700 MHz

Sample

#	Name	Isotope labeling	Concentration
8	Ubiquitin-fold modifier-conjugating enzyme 1	natural abundance	1.2 (±0.05) mM
9	Ubiquitin-like modifier-activating enzyme 5	[U-100% 13C; U-100% 15N]	0.3 (±0.05) mM
10	TRIS	natural abundance	50 (±1.0) mM
11	sodium chloride	natural abundance	100 (±1.0) mM
12	TCEP	natural abundance	2 (±0.1) mM
13	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
14	DSS	natural abundance	0.15 (±0.01) mM

14 3D NOESY [13C,1H]-HSQC

Instrument

Bruker AVANCE NEO - 900 MHz

Sample

#	Name	Isotope labeling	Concentration
8	Ubiquitin-fold modifier-conjugating enzyme 1	natural abundance	1.2 (±0.05) mM
9	Ubiquitin-like modifier-activating enzyme 5	[U-100% 13C; U-100% 15N]	0.3 (±0.05) mM
10	TRIS	natural abundance	50 (±1.0) mM
11	sodium chloride	natural abundance	100 (±1.0) mM
12	TCEP	natural abundance	2 (±0.1) mM
13	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
14	DSS	natural abundance	0.15 (±0.01) mM

15 3D [15N,1H]-TROSY-H(CC)(CO)NH-TOCSY

Instrument

Bruker AVANCE II - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
8	Ubiquitin-fold modifier-conjugating enzyme 1	natural abundance	1.2 (±0.05) mM
9	Ubiquitin-like modifier-activating enzyme 5	[U-100% 13C; U-100% 15N]	0.3 (±0.05) mM
10	TRIS	natural abundance	50 (±1.0) mM
11	sodium chloride	natural abundance	100 (±1.0) mM
12	TCEP	natural abundance	2 (±0.1) mM
13	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
14	DSS	natural abundance	0.15 (±0.01) mM

16 3D NOESY-[15N,1H]-SOFAST-HMQC

Instrument

Bruker AVANCE III HD - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
8	Ubiquitin-fold modifier-conjugating enzyme 1	natural abundance	1.2 (±0.05) mM
9	Ubiquitin-like modifier-activating enzyme 5	[U-100% 13C; U-100% 15N]	0.3 (±0.05) mM
10	TRIS	natural abundance	50 (±1.0) mM
11	sodium chloride	natural abundance	100 (±1.0) mM
12	TCEP	natural abundance	2 (±0.1) mM
13	AEBSF protease inhibitor	natural abundance	5 (±0.1) mM
14	DSS	natural abundance	0.15 (±0.01) mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34638_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34638_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	2268		96.3 (chain: 2, length: 27), 99.4 (chain: 1, length: 167)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 2, Chain length: 27, Sequence coverage: 96.3%
  - Entity_assembly_ID: 1, Chain length: 167, Sequence coverage: 99.4%
- Total number of assignments
  - ¹H chemical shifts: 1396
  - ¹³C chemical shifts: 676
  - ¹⁵N chemical shifts: 196
- Completeness of assignments
  - Entity_assemble_ID: 2
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 2
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	156	142	91.0
¹³C chemical shifts	117	81	69.2
¹⁵N chemical shifts	29	28	96.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	56	52	92.9
¹³C chemical shifts	54	26	48.1
¹⁵N chemical shifts	27	26	96.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	100	90	90.0
¹³C chemical shifts	63	55	87.3
¹⁵N chemical shifts	2	2	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	16	94.1
¹³C chemical shifts	17	16	94.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	6	1	16.7
¹³C chemical shifts	5	0	0.0
¹⁵N chemical shifts	1	1	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	1072	1034	96.5
¹³C chemical shifts	797	595	74.7
¹⁵N chemical shifts	186	168	90.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	334	322	96.4
¹³C chemical shifts	334	164	49.1
¹⁵N chemical shifts	157	149	94.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	738	712	96.5
¹³C chemical shifts	463	431	93.1
¹⁵N chemical shifts	29	19	65.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	94	94	100.0
¹³C chemical shifts	94	94	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	98	94	95.9
¹³C chemical shifts	92	67	72.8
¹⁵N chemical shifts	6	6	100.0

Keywords LIGASE, UBA5, UFC1, UFM1, complex structure, ufmylation

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Found 1 Document (1.895 seconds)

BMRB: 34638

Sample #1

Sample #2

Related entities 1. Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5, entity 1, : 1 : 13 entities Detail

Related entities 2. Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5, entity 2, : 1 : 3 : 9 entities Detail

Interaction partners 2. Ubiquitin-fold modifier-conjugating enzyme 1, Ubiquitin-like modifier-activating enzyme 5, entity 2, : 19 interactors Detail

Experiments performed 16 experiments Detail

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Chemical shift validation 4 contents Detail