BMRBj - BMREntryMaster

Found 1 Document (1.565 seconds)

BMRB: 34642

7OZ0

Solution structure of the N-terminal domain of human telomeric Repeat-binding factor 2-interacting protein 1 (hRap1): implication for Rap1-TRF2 interaction in Human.

Authors

Miron, S., LeDU, M.H., Gaullier, G., Zinn-justin, S., Cuniasse, P.

Assembly

Telomeric repeat-binding factor 2-interacting protein 1

Entity

1. Telomeric repeat-binding factor 2-interacting protein 1 (polymer), 114 monomers, 12125.45 Da Detail

MAEAMDLGKD PNGPTHSSTL FVRDDGSSMS FYVRPSPAKR RLSTLILHGG GTVCRVQEPG AVLLAQPGEA LAEASGDFIS TQYILDCVER NERLELEAYR LGPASAADTG SEAK

Formula weight

12125.45 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 88.4 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.4 % (1094 of 1238)	84.7 % (539 of 636)	92.9 % (448 of 482)	89.2 % (107 of 120)
Backbone	96.4 % (646 of 670)	95.7 % (222 of 232)	96.1 % (318 of 331)	99.1 % (106 of 107)
Sidechain	79.9 % (536 of 671)	77.0 % (311 of 404)	88.6 % (225 of 254)	0.0 % (0 of 13)
Aromatic	83.9 % (52 of 62)	83.9 % (26 of 31)	83.9 % (26 of 31)
Methyl	91.8 % (112 of 122)	91.8 % (56 of 61)	91.8 % (56 of 61)

1. entity 1

MAEAMDLGKD PNGPTHSSTL FVRDDGSSMS FYVRPSPAKR RLSTLILHGG GTVCRVQEPG AVLLAQPGEA LAEASGDFIS TQYILDCVER NERLELEAYR LGPASAADTG SEAK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 100 uM [U-100% 13C; U-100% 15N] human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

Release date

2021-07-29

Citation

Solution structure of the N-terminal domain of human telomeric Repeat-binding factor 2-interacting protein 1 (hRap1): implication for Rap1-TRF2 interaction in Human
Miron, S., LeDU, M.H., Gaullier, G., Zinn-justin, S., Cuniasse, P.

Related entities 1. Telomeric repeat-binding factor 2-interacting protein 1, : 1 : 2 : 6 entities Detail

Interaction partners 1. Telomeric repeat-binding factor 2-interacting protein 1, : 144 interactors Detail

More details for 144 interactors identified by 14 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE II - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 100 uM [U-100% 13C; U-100% 15N] human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1), 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

2 3D HNCACB

Instrument

Bruker AVANCE II - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

3 3D HNCO

Instrument

Bruker AVANCE II - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

4 3D HN(CA)CO

Instrument

Bruker AVANCE II - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

5 3D HCCH-TOSCY

Instrument

Bruker AVANCE II - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

6 3D 1H-15N TOCSY

Instrument

Bruker AVANCE II - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

7 3D 15N NOESY-HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

8 3D 13C (aromatic)NOESY-HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

9 3D 13C(aliphatic)NOESY-HSQC

Instrument

Bruker AVANCE III - 950 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

10 3D 1H-15N NOESY

Instrument

Bruker AVANCE II - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	human Telomeric Repeat-binding factor 2-interacting protein 1 (hRAP1)	[U-100% 13C; U-100% 15N]		100 (±10.0) uM
2	NaCl	natural abundance		150 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34642_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34642_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1209		99.1 (chain: 1, length: 114)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 114, Sequence coverage: 99.1%
- Total number of assignments
  - ¹H chemical shifts: 660
  - ¹³C chemical shifts: 442
  - ¹⁵N chemical shifts: 107
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	636	546	85.8
¹³C chemical shifts	482	442	91.7
¹⁵N chemical shifts	120	107	89.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	232	228	98.3
¹³C chemical shifts	228	217	95.2
¹⁵N chemical shifts	107	107	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	404	318	78.7
¹³C chemical shifts	254	225	88.6
¹⁵N chemical shifts	13	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	64	57	89.1
¹³C chemical shifts	64	57	89.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	31	26	83.9
¹³C chemical shifts	31	26	83.9

Keywords TRANSCRIPTION, Telomere Integrity

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Found 1 Document (1.565 seconds)

BMRB: 34642

Sample

Related entities 1. Telomeric repeat-binding factor 2-interacting protein 1, : 1 : 2 : 6 entities Detail

Interaction partners 1. Telomeric repeat-binding factor 2-interacting protein 1, : 144 interactors Detail

Experiments performed 10 experiments Detail

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Chemical shift validation 3 contents Detail