BMRB: 34645

Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles

Authors

Wang, X., Bouaziz, S.

Assembly

Gag polyprotein

Entity

1. Gag polyprotein (polymer, Thiol state: not present), 13 monomers, 1535.716 Da Detail

EDPAVDLLKN YMQ

Formula weight

1535.716 Da

Source organism

Simian immunodeficiency virus - mac K6W

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 91.5 %, Completeness (bb): 92.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	91.5 % (75 of 82)	91.5 % (75 of 82)
Backbone	92.0 % (23 of 25)	92.0 % (23 of 25)
Sidechain	91.2 % (52 of 57)	91.2 % (52 of 57)
Aromatic	100.0 % (4 of 4)	100.0 % (4 of 4)
Methyl	100.0 % (7 of 7)	100.0 % (7 of 7)

1. entity 1

EDPAVDLLKN YMQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 4.0, Details 1.0 mM unlabeled SIVmac p6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SIVmac p6	natural abundance		1.0 mM

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Release date

2021-08-01

Citation

Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles
Wang, X.

Related entities 1. Gag polyprotein, : 1 : 2 : 10 entities Detail

Interaction partners 1. Gag polyprotein, : 1 interactors Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail