BMRBj - BMREntryMaster

Found 1 Document (0.457 seconds)

BMRB: 34646

5O2U

Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles

Authors

Wang, X., Bouaziz, S.

Assembly

Gag protein

Entity

1. Gag protein (polymer, Thiol state: not present), 52 monomers, 5807.257 Da Detail

LQSRPEPTAP PEESFRFGEE TTTPSQKQEP IDKELYPLAS LRSLFGSDPS SQ

Formula weight

5807.257 Da

Source organism

Human immunodeficiency virus 1

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.4 %, Completeness: 71.0 %, Completeness (bb): 71.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	71.0 % (265 of 373)	79.2 % (255 of 322)	19.6 % (10 of 51)
Backbone	71.1 % (101 of 142)	92.9 % (91 of 98)	22.7 % (10 of 44)
Sidechain	71.0 % (164 of 231)	73.2 % (164 of 224)	0.0 % (0 of 7)
Aromatic	73.7 % (14 of 19)	73.7 % (14 of 19)
Methyl	83.3 % (15 of 18)	83.3 % (15 of 18)

1. entity 1

LQSRPEPTAP PEESFRFGEE TTTPSQKQEP IDKELYPLAS LRSLFGSDPS SQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 4, Details 1.0 mM unlabeled HIV-1 p6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 p6	natural abundance		1.0 mM

Release date

2021-08-01

Citation

Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles
Wang, X., Bouaziz, S.

Related entities 1. Gag protein, : 1 : 2 : 1 : 1 : 41 entities Detail

Interaction partners 1. Gag protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34646_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34646_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	291		90.4 (chain: 1, length: 52)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 52, Sequence coverage: 90.4%
- Total number of assignments
  - ¹H chemical shifts: 285
  - ¹⁵N chemical shifts: 6
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	322	255	79.2
¹⁵N chemical shifts	51	6	11.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	98	91	92.9
¹⁵N chemical shifts	44	6	13.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	224	164	73.2
¹⁵N chemical shifts	7	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	18	15	83.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	14	73.7

Keywords HIV-1, P6, VIRAL PROTEIN

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Found 1 Document (0.457 seconds)

BMRB: 34646

Sample

Related entities 1. Gag protein, : 1 : 2 : 1 : 1 : 41 entities Detail

Interaction partners 1. Gag protein, : 1 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail