BMRBj - BMREntryMaster

Found 1 Document (0.347 seconds)

BMRB: 34647

Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles

Authors

Wang, X., Bouaziz, S.

Assembly

Gag polyprotein

Entity

1. Gag polyprotein (polymer, Thiol state: not present), 63 monomers, 7232.102 Da Detail

PMAQVHQGLM PTAPPEDPAV DLLKNYMQLG KQQREKQRES REKPYKEVTE DLLHLNSLFG GDQ

Formula weight

7232.102 Da

Source organism

Simian immunodeficiency virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.1 %, Completeness: 43.3 %, Completeness (bb): 44.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.3 % (329 of 760)	67.2 % (272 of 405)	9.1 % (26 of 286)	44.9 % (31 of 69)
Backbone	44.8 % (164 of 366)	92.7 % (115 of 124)	10.3 % (19 of 185)	52.6 % (30 of 57)
Sidechain	37.5 % (170 of 453)	55.9 % (157 of 281)	7.5 % (12 of 160)	8.3 % (1 of 12)
Aromatic	58.8 % (20 of 34)	76.5 % (13 of 17)	41.2 % (7 of 17)
Methyl	38.9 % (21 of 54)	77.8 % (21 of 27)	0.0 % (0 of 27)

1. entity 1

PMAQVHQGLM PTAPPEDPAV DLLKNYMQLG KQQREKQRES REKPYKEVTE DLLHLNSLFG GDQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 323 K, pH 4, Details 1 mM SIVmac p6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SIVmac p6	natural abundance		1 mM

Release date

2021-08-01

Citation

Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles
Wang, X., Bouaziz, S.

Related entities 1. Gag polyprotein, : 24 entities Detail

Interaction partners 1. Gag polyprotein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34647_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34647_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	359		92.1 (chain: 1, length: 63)
1	Chemical shift references	chem_shift_reference_1	OK	1		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 63, Sequence coverage: 92.1%
- Total number of assignments
  - ¹H chemical shifts: 305
  - ¹⁵N chemical shifts: 31
  - ¹³C chemical shifts: 23
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	405	263	64.9
¹⁵N chemical shifts	69	31	44.9
¹³C chemical shifts	286	23	8.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	124	116	93.5
¹⁵N chemical shifts	57	30	52.6
¹³C chemical shifts	126	12	9.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	281	147	52.3
¹⁵N chemical shifts	12	1	8.3
¹³C chemical shifts	160	11	6.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	30	21	70.0
¹³C chemical shifts	30	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	17	13	76.5
¹³C chemical shifts	17	7	41.2

Keywords SIVmac p6, VIRAL PROTEIN

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Found 1 Document (0.347 seconds)

BMRB: 34647

Sample

Related entities 1. Gag polyprotein, : 24 entities Detail

Interaction partners 1. Gag polyprotein, : 1 interactors Detail

Experiments performed 2 experiments Detail

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail