BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	82.6 % (526 of 637)	78.5 % (263 of 335)	88.3 % (211 of 239)	82.5 % (52 of 63)
Backbone	85.0 % (267 of 314)	80.2 % (85 of 106)	87.3 % (137 of 157)	88.2 % (45 of 51)
Sidechain	81.8 % (306 of 374)	77.7 % (178 of 229)	91.0 % (121 of 133)	58.3 % (7 of 12)
Aromatic	71.4 % (30 of 42)	71.4 % (15 of 21)	75.0 % (15 of 20)	0.0 % (0 of 1)
Methyl	95.8 % (46 of 48)	91.7 % (22 of 24)	100.0 % (24 of 24)

1. entity 1

GSWGSMENVN YDLLQKQVKY IMDSNMLNLP QFQHLPQEEK MSAILAMLNS NSD

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM [U-100% 13C; U-100% 15N] Nab3, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	25 mM
3	DTT	natural abundance	1 mM
4	Nab3	[U-100% 13C; U-100% 15N]	480 uM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM Nab3, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
5	potassium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	25 mM
7	DTT	natural abundance	1 mM
8	Nab3	natural abundance	480 uM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM Nab3, 100% D2O

#	Name	Isotope labeling	Concentration
9	potassium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	25 mM
11	DTT	natural abundance	1 mM
12	Nab3	natural abundance	480 uM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM [U-100% 13C; U-100% 15N] Nab3, 100% D2O

#	Name	Isotope labeling	Concentration
13	potassium phosphate	natural abundance	25 mM
14	sodium chloride	natural abundance	25 mM
15	DTT	natural abundance	1 mM
16	Nab3	[U-100% 13C; U-100% 15N]	480 uM

Release date

2021-10-14

Citation

Structural basis of Nrd1-Nab3 heterodimerization
Chaves-Arquero, B., Martinez-Lumbreras, S., Camero, S., Santiveri, C.M., Mirassou, Y., Campos-Olivas, R., Jimenez, M.A., Calvo, O., Perez-Canadillas, J.M.
Life Sci. Alliance (2022), 5, e202101252-e202101252, PubMed 35022249 , DOI 10.26508/lsa.202101252 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 34668 released on 2021-10-14
Title Solution structure of the chimeric Nrd1-Nab3 heterodimerization domains

Related entities 1. HLJ1 G0022400.mRNA.1.CDS.1, : 2 : 3 entities Detail

Interaction partners 1. HLJ1 G0022400.mRNA.1.CDS.1, : 33 interactors Detail

More details for 33 interactors identified by 8 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM [U-100% 13C; U-100% 15N] Nab3, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	25 mM
3	DTT	natural abundance	1 mM
4	Nab3	[U-100% 13C; U-100% 15N]	480 uM

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM [U-100% 13C; U-100% 15N] Nab3, 100% D2O

#	Name	Isotope labeling	Concentration
13	potassium phosphate	natural abundance	25 mM
14	sodium chloride	natural abundance	25 mM
15	DTT	natural abundance	1 mM
16	Nab3	[U-100% 13C; U-100% 15N]	480 uM

3 3D HNCA

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	25 mM
3	DTT	natural abundance	1 mM
4	Nab3	[U-100% 13C; U-100% 15N]	480 uM

4 3D HNCO

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	25 mM
3	DTT	natural abundance	1 mM
4	Nab3	[U-100% 13C; U-100% 15N]	480 uM

5 3D CBCA(CO)NH

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	25 mM
3	DTT	natural abundance	1 mM
4	Nab3	[U-100% 13C; U-100% 15N]	480 uM

6 3D HNCACB

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	potassium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	25 mM
3	DTT	natural abundance	1 mM
4	Nab3	[U-100% 13C; U-100% 15N]	480 uM

7 3D HCCH-TOCSY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
13	potassium phosphate	natural abundance	25 mM
14	sodium chloride	natural abundance	25 mM
15	DTT	natural abundance	1 mM
16	Nab3	[U-100% 13C; U-100% 15N]	480 uM

8 2D 1H-1H NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 308 K, pH 6.6, Details 25 mM potassium phosphate, 25 mM sodium chloride, 1 mM DTT, 480 uM Nab3, 100% D2O

#	Name	Isotope labeling	Concentration
9	potassium phosphate	natural abundance	25 mM
10	sodium chloride	natural abundance	25 mM
11	DTT	natural abundance	1 mM
12	Nab3	natural abundance	480 uM

9 2D 1H-1H NOESY

Instrument

Bruker AVANCE - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
5	potassium phosphate	natural abundance	25 mM
6	sodium chloride	natural abundance	25 mM
7	DTT	natural abundance	1 mM
8	Nab3	natural abundance	480 uM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34669_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34669_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	611		90.6 (chain: 1, length: 53)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 53, Sequence coverage: 90.6%
- Total number of assignments
  - ¹H chemical shifts: 342
  - ¹³C chemical shifts: 217
  - ¹⁵N chemical shifts: 52
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	335	284	84.8
¹³C chemical shifts	239	217	90.8
¹⁵N chemical shifts	63	52	82.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	106	92	86.8
¹³C chemical shifts	106	93	87.7
¹⁵N chemical shifts	51	45	88.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	229	192	83.8
¹³C chemical shifts	133	124	93.2
¹⁵N chemical shifts	12	7	58.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	29	29	100.0
¹³C chemical shifts	29	29	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	21	15	71.4
¹³C chemical shifts	20	15	75.0
¹⁵N chemical shifts	1	0	0.0

Keywords NNS pathway, Nab3, TRANSCRIPTION, Transcription termination, heterodimerization domain, yeast

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Found 1 Document (1.073 seconds)

BMRB: 34669

Sample #1

Sample #2

Sample #3

Sample #4

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. HLJ1 G0022400.mRNA.1.CDS.1, : 2 : 3 entities Detail

Interaction partners 1. HLJ1 G0022400.mRNA.1.CDS.1, : 33 interactors Detail

Experiments performed 9 experiments Detail

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Chemical shift validation 3 contents Detail