BMRB: 34671

Structure of Viscotoxin A3 from Viscum Album in the complex with DPC micelles

Authors

Paramonov, A.S., Shenkarev, Z.O.

Assembly

Viscotoxin-A3

Entity

1. Viscotoxin-A3 (polymer, Thiol state: all disulfide bound), 46 monomers, 4835.569 Da Detail

KSCCPNTTGR NIYNACRLTG APRPTCAKLS GCKIISGSTC PSDYPK

Formula weight

4835.569 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS40:SG
2	disulfide	sing	1:CYS4:SG	1:CYS32:SG
3	disulfide	sing	1:CYS16:SG	1:CYS26:SG

Source organism

Viscum album

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.2 %, Completeness (bb): 98.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.2 % (258 of 260)	99.2 % (258 of 260)
Backbone	98.9 % (90 of 91)	98.9 % (90 of 91)
Sidechain	99.4 % (168 of 169)	99.4 % (168 of 169)
Aromatic	100.0 % (8 of 8)	100.0 % (8 of 8)
Methyl	100.0 % (18 of 18)	100.0 % (18 of 18)

1. entity 1

KSCCPNTTGR NIYNACRLTG APRPTCAKLS GCKIISGSTC PSDYPK

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 4.0 (±0.1), Details 1.6 mM VtA3, 128 mM [U-100% 2H] DPC, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	VtA3	natural abundance		1.6 (±0.1) mM
2	DPC	[U-100% 2H]		128 (±1.0) mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 4.0 (±0.1), Details 1.6 mM VtA3, 128 mM [U-100% 2H] DPC, 100% D2O

#	Name	Isotope labeling	Type	Concentration
3	VtA3	natural abundance		1.6 (±0.1) mM
4	DPC	[U-100% 2H]		128 (±1.0) mM

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Release date

2021-10-25

Citation

Spatial structure and oligomerization of viscotoxin A3 in detergent micelles: Implication for mechanisms of ion channel formation and membrane lysis
Paramonov, A.S., Lyukmanova, E.N., Tonevitsky, A.G., Arseniev, A.S., Shenkarev, Z.O.
Biochem. Biophys. Res. Commun. (2021), 585, 22-28, PubMed 34781057 , DOI 10.1016/j.bbrc.2021.11.022 , Abstract

Related entities 1. Viscotoxin-A3, : 1 : 3 : 5 : 66 entities Detail

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail