BMRB: 34674

Solution structure of an intramolecular RNA G-quadruplex formed by the 6A8U17U mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto onco-gene

Authors

Wang, Z., Jurt, S., Dominguez-Martin, A., Johannsen, S., Sigel, R.K.O.

Assembly

RNA (5'-R(*GP*GP*GP*CP*CP*AP*UP*UP*GP*GP*GP*UP*GP*GP*GP*AP*UP*CP*UP*GP*GP*G)-3')

Entity

1. RNA (5'-R(*GP*GP*GP*CP*CP*AP*UP*UP*GP*GP*GP*UP*GP*GP*GP*AP*UP*CP*UP*GP*GP*G)-3') (polymer, Thiol state: not present), 22 monomers, 7265.269 Da Detail

GGGCCAUUGG GUGGGAUCUG GG

Formula weight

7265.269 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.5 %, Completeness (bb): 99.6 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	96.5 % (334 of 346)	94.1 % (176 of 187)	99.3 % (141 of 142)	100.0 % (17 of 17)
Suger, PO4	99.6 % (241 of 242)	100.0 % (132 of 132)	99.1 % (109 of 110)
Nucleobase	89.4 % (93 of 104)	80.0 % (44 of 55)	100.0 % (32 of 32)	100.0 % (17 of 17)
Aromatic	94.9 % (93 of 98)	89.8 % (44 of 49)	100.0 % (32 of 32)	100.0 % (17 of 17)

1. entity 1

GGGCCAUUGG GUGGGAUCUG GG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.0 mM BCL-2 6A8U17U, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	BCL-2 6A8U17U	natural abundance		1.0 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1.0 mM BCL-2 6A8U17U, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	BCL-2 6A8U17U	natural abundance		1.0 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM G & U 15N-labeled BCL-2 6A8U17U, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	BCL-2 6A8U17U	[G & U 15N-labeled]		0.5 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM G & U 15N-labeled BCL-2 6A8U17U, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	BCL-2 6A8U17U	[G & U 15N-labeled]		0.5 mM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM A & C 15N-labeled BCL-2 6A8U17U, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	BCL-2 6A8U17U	[A & C 15N-labeled]		0.5 mM

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM A 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	BCL-2 6A8U17U	[A 13C, 15N-labeled]		0.5 mM

Sample #7

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.3 mM C 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	BCL-2 6A8U17U	[C 13C, 15N-labeled]		0.3 mM

Sample #8

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.3 mM G 13C-labeled BCL-2 6A8U17U, 100% D2O

#	Name	Isotope labeling	Type	Concentration
8	BCL-2 6A8U17U	[G 13C-labeled]		0.3 mM

Sample #9

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.5 mM U 13C, 15N-labeled BCL-2 6A8U17U, 100% D2O

#	Name	Isotope labeling	Type	Concentration
9	BCL-2 6A8U17U	[U 13C, 15N-labeled]		0.5 mM

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Release date

2021-11-28

Citation

Solution structure of an intramolecular RNA G-quadruplex formed by the 6A8U17U mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto onco-gene
Wang, Z., Jurt, S., Dominguez-Martin, A., Johannsen, S., Sigel, R.K.O.

Experiments performed 15 experiments Detail

Chemical shift validation 3 contents Detail