BMRB: 34677

Solution structure of an intramolecular RNA G-quadruplex formed by the 6A mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto-oncogene

Authors

Wang, Z., Falk, T., Jurt, S., Johannsen, S., Sigel, R.K.O.

Assembly

RNA (5'-R(*GP*GP*GP*CP*CP*AP*UP*GP*GP*GP*GP*UP*GP*GP*GP*AP*GP*CP*UP*GP*GP*G)-3')

Entity

1. RNA (5'-R(*GP*GP*GP*CP*CP*AP*UP*GP*GP*GP*GP*UP*GP*GP*GP*AP*GP*CP*UP*GP*GP*G)-3') (polymer, Thiol state: not present), 22 monomers, 7343.349 Da Detail

GGGCCAUGGG GUGGGAGCUG GG

Formula weight

7343.349 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.1 %, Completeness (bb): 93.4 % Detail

Download

• NMR-STAR v3.1 (partial)
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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	87.1 % (298 of 342)	91.4 % (169 of 185)	92.1 % (129 of 140)	0.0 % (0 of 17)
Suger, PO4	93.4 % (226 of 242)	96.2 % (127 of 132)	90.0 % (99 of 110)
Nucleobase	72.0 % (72 of 100)	79.2 % (42 of 53)	100.0 % (30 of 30)	0.0 % (0 of 17)
Aromatic	76.6 % (72 of 94)	89.4 % (42 of 47)	100.0 % (30 of 30)	0.0 % (0 of 17)

1. entity 1

GGGCCAUGGG GUGGGAGCUG GG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.6 mM BCL-2 6A, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	BCL-2 6A	natural abundance		0.6 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.6 mM BCL-2 6A, 100% D2O

#	Name	Isotope labeling	Type	Concentration
2	BCL-2 6A	natural abundance		0.6 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.15 mM [U-13C; U-15N]-Gua BCL-2 6A, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
3	BCL-2 6A	[U-13C; U-15N]-Gua		0.15 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.4 mM [U-13C; U-15N]-Ade BCL-2 6A, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	BCL-2 6A	[U-13C; U-15N]-Ade		0.4 mM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.15 mM [U-13C; U-15N]-Gua BCL-2 6A, 100% D2O

#	Name	Isotope labeling	Type	Concentration
5	BCL-2 6A	[U-13C; U-15N]-Gua		0.15 mM

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 0.4 mM [U-13C; U-15N]-Ura BCL-2 6A, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	BCL-2 6A	[U-13C; U-15N]-Ura		0.4 mM

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Release date

2021-12-13

Citation

Solution structure of an intramolecular RNA G-quadruplex formed by the 6A mutant from a 22mer guanine-rich sequence within the 5'UTR of BCL-2 proto-oncogene
Wang, Z., Falk, T., Jurt, S., Johannsen, S., Sigel, R.K.O.

Related entities 1. RNA (5'-R(*GP*GP*GP*CP*CP*AP*UP*GP*GP*GP*GP*UP*GP*GP*GP*AP*GP*CP*UP*GP*GP*G)-3'), : 1 entities Detail

Experiments performed 10 experiments Detail