BMRB: 34680

Three-dimensional structure of the PGAM5 C12L mutant TMD

Authors

Silber, M., Muhle-Goll, C.

Assembly

Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16)

Entity

1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16) (polymer, Thiol state: not present), 35 monomers, 3311.790 Da Detail

AFRQALQLAA LGLAGGSAAV LFSAVAVGKP RAGGD

Formula weight

3311.79 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 170, ¹³C: 79 assignments Detail

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	ALA	HA	H	4.077
2	1	1	ALA	HB1	H	1.636
3	1	1	ALA	HB2	H	1.636
4	1	1	ALA	HB3	H	1.636
5	1	1	ALA	CA	C	51.353
6	1	1	ALA	CB	C	17.673
7	1	2	PHE	H	H	7.918
8	1	2	PHE	HA	H	4.515
9	1	2	PHE	HB2	H	3.217
10	1	2	PHE	CA	C	59.048
11	1	2	PHE	CB	C	37.804
12	1	3	ARG	H	H	8.328
13	1	3	ARG	HA	H	3.975
14	1	3	ARG	HB2	H	1.846
15	1	3	ARG	HB3	H	1.914
16	1	3	ARG	HG2	H	1.647
17	1	3	ARG	HG3	H	1.718
18	1	3	ARG	HD2	H	3.208
19	1	3	ARG	CA	C	58.073
20	1	3	ARG	CB	C	28.608
21	1	3	ARG	CG	C	26.287
22	1	3	ARG	CD	C	42.082
23	1	4	GLN	H	H	7.942
24	1	4	GLN	HA	H	4.112
25	1	4	GLN	HB2	H	2.07
26	1	4	GLN	HB3	H	2.196
27	1	4	GLN	HG2	H	2.392
28	1	4	GLN	HG3	H	2.484
29	1	4	GLN	CA	C	58.122
30	1	4	GLN	CB	C	26.974
31	1	4	GLN	CG	C	33.313
32	1	5	ALA	H	H	7.655
33	1	5	ALA	HA	H	4.087
34	1	5	ALA	HB1	H	1.477
35	1	5	ALA	HB2	H	1.477
36	1	5	ALA	HB3	H	1.477
37	1	5	ALA	CA	C	53.939
38	1	5	ALA	CB	C	16.402
39	1	6	LEU	H	H	7.834
40	1	6	LEU	HA	H	4.076
41	1	6	LEU	HB2	H	1.579
42	1	6	LEU	HB3	H	1.679
43	1	6	LEU	HG	H	1.622
44	1	6	LEU	CA	C	56.517
45	1	6	LEU	CB	C	40.57
46	1	6	LEU	CG	C	25.705
47	1	7	GLN	H	H	7.796
48	1	7	GLN	HA	H	4.043
49	1	7	GLN	HB2	H	2.231
50	1	7	GLN	HB3	H	2.154
51	1	7	GLN	HG2	H	2.501
52	1	7	GLN	HG3	H	2.352
53	1	7	GLN	CA	C	57.801
54	1	7	GLN	CB	C	27.435
55	1	7	GLN	CG	C	33.024
56	1	8	LEU	H	H	7.974
57	1	8	LEU	HA	H	4.157
58	1	8	LEU	HB2	H	1.583
59	1	8	LEU	HB3	H	1.886
60	1	8	LEU	HG	H	1.805
61	1	8	LEU	CA	C	56.676
62	1	8	LEU	CB	C	40.527
63	1	8	LEU	CG	C	25.833
64	1	9	ALA	H	H	8.11
65	1	9	ALA	HA	H	4.123
66	1	9	ALA	HB1	H	1.527
67	1	9	ALA	HB2	H	1.527
68	1	9	ALA	HB3	H	1.527
69	1	9	ALA	CA	C	53.866
70	1	9	ALA	CB	C	16.129
71	1	10	ALA	H	H	8.154
72	1	10	ALA	HA	H	4.089
73	1	10	ALA	HB1	H	1.53
74	1	10	ALA	HB2	H	1.53
75	1	10	ALA	HB3	H	1.53
76	1	10	ALA	CA	C	53.939
77	1	10	ALA	CB	C	16.436
78	1	11	LEU	H	H	8.004
79	1	11	LEU	HA	H	4.175
80	1	11	LEU	HB2	H	1.647
81	1	11	LEU	HB3	H	1.911
82	1	11	LEU	HG	H	1.802
83	1	11	LEU	CA	C	56.316
84	1	11	LEU	CB	C	40.906
85	1	11	LEU	CG	C	25.833
86	1	12	GLY	H	H	8.158
87	1	12	GLY	HA2	H	3.91
88	1	12	GLY	HA3	H	3.912
89	1	12	GLY	CA	C	45.335
90	1	13	LEU	H	H	8.056
91	1	13	LEU	HA	H	4.289
92	1	13	LEU	HB2	H	1.645
93	1	13	LEU	HB3	H	1.858
94	1	13	LEU	HG	H	1.761
95	1	13	LEU	CA	C	55.856
96	1	13	LEU	CB	C	40.929
97	1	13	LEU	CG	C	25.765
98	1	14	ALA	H	H	8.171
99	1	14	ALA	HA	H	4.22
100	1	14	ALA	HB1	H	1.514
101	1	14	ALA	HB2	H	1.514
102	1	14	ALA	HB3	H	1.514
103	1	14	ALA	CA	C	53.086
104	1	14	ALA	CB	C	16.702
105	1	15	GLY	H	H	8.254
106	1	15	GLY	HA2	H	3.958
107	1	15	GLY	CA	C	45.033
108	1	16	GLY	H	H	8.17
109	1	16	GLY	HA3	H	3.999
110	1	16	GLY	CA	C	45.019
111	1	17	SER	H	H	8.013
112	1	17	SER	HA	H	4.286
113	1	17	SER	HB2	H	3.959
114	1	17	SER	HB3	H	4.024
115	1	17	SER	CA	C	59.768
116	1	17	SER	CB	C	62.024
117	1	18	ALA	H	H	7.935
118	1	18	ALA	HA	H	4.093
119	1	18	ALA	HB1	H	1.472
120	1	18	ALA	HB2	H	1.472
121	1	18	ALA	HB3	H	1.472
122	1	18	ALA	CA	C	53.93
123	1	18	ALA	CB	C	16.402
124	1	19	ALA	H	H	7.692
125	1	19	ALA	HA	H	4.144
126	1	19	ALA	HB1	H	1.529
127	1	19	ALA	HB2	H	1.529
128	1	19	ALA	HB3	H	1.529
129	1	19	ALA	CA	C	53.926
130	1	19	ALA	CB	C	16.825
131	1	20	VAL	H	H	7.687
132	1	20	VAL	HA	H	3.696
133	1	20	VAL	HB	H	2.227
134	1	20	VAL	HG11	H	0.966
135	1	20	VAL	HG12	H	0.966
136	1	20	VAL	HG13	H	0.966
137	1	20	VAL	HG21	H	1.077
138	1	20	VAL	HG22	H	1.077
139	1	20	VAL	HG23	H	1.077
140	1	20	VAL	CA	C	65.08
141	1	20	VAL	CB	C	30.738
142	1	21	LEU	H	H	7.791
143	1	21	LEU	HA	H	4.117
144	1	21	LEU	HB2	H	1.63
145	1	21	LEU	HB3	H	1.684
146	1	21	LEU	CA	C	56.964
147	1	21	LEU	CB	C	40.582
148	1	22	PHE	H	H	8.412
149	1	22	PHE	HA	H	4.348
150	1	22	PHE	HB2	H	3.202
151	1	22	PHE	CA	C	59.325
152	1	22	PHE	CB	C	37.495
153	1	23	SER	H	H	7.991
154	1	23	SER	HA	H	4.189
155	1	23	SER	HB2	H	4.027
156	1	23	SER	HB3	H	4.111
157	1	23	SER	CA	C	60.536
158	1	23	SER	CB	C	62.018
159	1	24	ALA	H	H	7.998
160	1	24	ALA	HA	H	4.182
161	1	24	ALA	HB1	H	1.533
162	1	24	ALA	HB2	H	1.533
163	1	24	ALA	HB3	H	1.533
164	1	24	ALA	CA	C	53.578
165	1	24	ALA	CB	C	16.458
166	1	25	VAL	H	H	7.87
167	1	25	VAL	HA	H	3.835
168	1	25	VAL	HB	H	2.163
169	1	25	VAL	HG11	H	0.937
170	1	25	VAL	HG12	H	0.937
171	1	25	VAL	HG13	H	0.937
172	1	25	VAL	HG21	H	1.039
173	1	25	VAL	HG22	H	1.039
174	1	25	VAL	HG23	H	1.039
175	1	25	VAL	CA	C	63.55
176	1	25	VAL	CB	C	31.033
177	1	26	ALA	H	H	7.936
178	1	26	ALA	HA	H	4.12
179	1	26	ALA	HB1	H	1.327
180	1	26	ALA	HB2	H	1.327
181	1	26	ALA	HB3	H	1.327
182	1	26	ALA	CA	C	53.034
183	1	26	ALA	CB	C	17.299
184	1	27	VAL	H	H	7.633
185	1	27	VAL	HA	H	4.002
186	1	27	VAL	HB	H	2.204
187	1	27	VAL	HG11	H	0.977
188	1	27	VAL	HG12	H	0.977
189	1	27	VAL	HG13	H	0.977
190	1	27	VAL	HG21	H	1.02
191	1	27	VAL	HG22	H	1.02
192	1	27	VAL	HG23	H	1.02
193	1	27	VAL	CA	C	62.533
194	1	27	VAL	CB	C	31.058
195	1	28	GLY	H	H	7.942
196	1	28	GLY	HA2	H	3.926
197	1	28	GLY	CA	C	44.228
198	1	29	LYS	H	H	7.686
199	1	29	LYS	HA	H	4.625
200	1	29	LYS	HB2	H	1.845
201	1	29	LYS	HG2	H	1.493
202	1	29	LYS	HD2	H	1.711
203	1	29	LYS	HE2	H	3.018
204	1	29	LYS	CA	C	53.388
205	1	29	LYS	CB	C	31.243
206	1	29	LYS	CG	C	23.058
207	1	29	LYS	CD	C	27.759
208	1	29	LYS	CE	C	41.189
209	1	30	PRO	HA	H	4.411
210	1	30	PRO	HB2	H	1.914
211	1	30	PRO	HB3	H	2.283
212	1	30	PRO	HG2	H	1.997
213	1	30	PRO	HG3	H	2.061
214	1	30	PRO	HD2	H	3.628
215	1	30	PRO	HD3	H	3.83
216	1	30	PRO	CA	C	62.435
217	1	30	PRO	CB	C	30.475
218	1	30	PRO	CG	C	25.976
219	1	30	PRO	CD	C	49.254
220	1	31	ARG	H	H	7.927
221	1	31	ARG	HA	H	4.409
222	1	31	ARG	HB2	H	1.79
223	1	31	ARG	HB3	H	1.923
224	1	31	ARG	HG2	H	1.685
225	1	31	ARG	HD2	H	3.212
226	1	31	ARG	CA	C	54.56
227	1	31	ARG	CB	C	29.874
228	1	31	ARG	CG	C	25.806
229	1	31	ARG	CD	C	42.082
230	1	32	ALA	H	H	7.984
231	1	32	ALA	HA	H	4.347
232	1	32	ALA	HB1	H	1.406
233	1	32	ALA	HB2	H	1.406
234	1	32	ALA	HB3	H	1.406
235	1	32	ALA	CA	C	51.477
236	1	32	ALA	CB	C	17.549
237	1	33	GLY	H	H	8.067
238	1	33	GLY	HA2	H	3.897
239	1	33	GLY	HA3	H	4.031
240	1	33	GLY	CA	C	44.063
241	1	34	GLY	H	H	7.994
242	1	34	GLY	HA2	H	3.964
243	1	34	GLY	CA	C	43.991
244	1	35	ASP	H	H	7.78
245	1	35	ASP	HA	H	4.507
246	1	35	ASP	HB2	H	2.729
247	1	35	ASP	HB3	H	2.682
248	1	35	ASP	CA	C	53.7
249	1	35	ASP	CB	C	40.38

Release date

2022-04-25

Citation

Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state
Siebert, V., Silber, M., Heuten, E., Muhle-Goll, C., Lemberg, M.K.
J. Biol. Chem. (2022), 298, 102321-102321, PubMed 35921890 , DOI 10.1016/j.jbc.2022.102321 , Abstract

Related entities 1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16), : 6 : 1 entities Detail

Interaction partners 1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16), : 56 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail