BMRB: 34681

Three-dimensional structure of the PGAM5 WT TMD

Authors

Silber, M., Muhle-Goll, C.

Assembly

Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16)

Entity

1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16) (polymer), 35 monomers, 3301.775 Da Detail

AFRQALQLAA CGLAGGSAAV LFSAVAVGKP RAGGD

Formula weight

3301.775 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 193, ¹³C: 89 assignments Detail

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	ALA	HA	H	4.167
2	1	1	ALA	HB1	H	1.377
3	1	1	ALA	HB2	H	1.377
4	1	1	ALA	HB3	H	1.377
5	1	1	ALA	CA	C	52.970
6	1	1	ALA	CB	C	16.484
7	1	2	PHE	H	H	7.338
8	1	2	PHE	HA	H	4.453
9	1	2	PHE	HB2	H	3.133
10	1	2	PHE	HB3	H	3.185
11	1	2	PHE	CA	C	57.643
12	1	2	PHE	CB	C	37.228
13	1	3	ARG	H	H	7.425
14	1	3	ARG	HA	H	3.976
15	1	3	ARG	HB2	H	1.791
16	1	3	ARG	HB3	H	1.840
17	1	3	ARG	HG2	H	1.549
18	1	3	ARG	HG3	H	1.592
19	1	3	ARG	CA	C	57.889
20	1	3	ARG	CB	C	28.489
21	1	3	ARG	CG	C	26.163
22	1	3	ARG	CD	C	42.056
23	1	4	GLN	H	H	7.892
24	1	4	GLN	HA	H	4.129
25	1	4	GLN	HB2	H	2.163
26	1	4	GLN	HB3	H	2.161
27	1	4	GLN	HG2	H	2.394
28	1	4	GLN	HG3	H	2.394
29	1	4	GLN	CA	C	57.674
30	1	4	GLN	CB	C	27.215
31	1	4	GLN	CG	C	32.876
32	1	5	ALA	H	H	7.764
33	1	5	ALA	HA	H	4.113
34	1	5	ALA	HB1	H	1.529
35	1	5	ALA	HB2	H	1.529
36	1	5	ALA	HB3	H	1.529
37	1	5	ALA	CA	C	53.930
38	1	5	ALA	CB	C	16.300
39	1	6	LEU	H	H	7.868
40	1	6	LEU	HA	H	4.134
41	1	6	LEU	HB2	H	1.815
42	1	6	LEU	HB3	H	1.635
43	1	6	LEU	HG	H	1.780
44	1	6	LEU	HD11	H	0.936
45	1	6	LEU	HD12	H	0.936
46	1	6	LEU	HD13	H	0.936
47	1	6	LEU	CA	C	56.652
48	1	6	LEU	CB	C	40.573
49	1	6	LEU	CG	C	25.839
50	1	6	LEU	CD1	C	22.918
51	1	7	GLN	H	H	7.834
52	1	7	GLN	HA	H	4.066
53	1	7	GLN	HB2	H	2.226
54	1	7	GLN	HB3	H	2.222
55	1	7	GLN	HG2	H	2.389
56	1	7	GLN	HG3	H	2.508
57	1	7	GLN	CA	C	57.792
58	1	7	GLN	CB	C	27.334
59	1	7	GLN	CG	C	32.857
60	1	8	LEU	H	H	8.032
61	1	8	LEU	HA	H	4.145
62	1	8	LEU	HB2	H	1.627
63	1	8	LEU	HB3	H	1.910
64	1	8	LEU	HG	H	1.806
65	1	8	LEU	HD11	H	0.895
66	1	8	LEU	HD12	H	0.895
67	1	8	LEU	HD13	H	0.895
68	1	8	LEU	CA	C	56.832
69	1	8	LEU	CB	C	40.558
70	1	8	LEU	CG	C	25.859
71	1	8	LEU	CD1	C	22.318
72	1	9	ALA	H	H	8.164
73	1	9	ALA	HA	H	4.141
74	1	9	ALA	HB1	H	1.548
75	1	9	ALA	HB2	H	1.548
76	1	9	ALA	HB3	H	1.548
77	1	9	ALA	CA	C	53.851
78	1	9	ALA	CB	C	16.566
79	1	10	ALA	H	H	8.294
80	1	10	ALA	HA	H	4.139
81	1	10	ALA	HB1	H	1.564
82	1	10	ALA	HB2	H	1.564
83	1	10	ALA	HB3	H	1.564
84	1	10	ALA	CA	C	53.851
85	1	10	ALA	CB	C	16.532
86	1	11	CYS	H	H	8.083
87	1	11	CYS	HA	H	4.293
88	1	11	CYS	HB2	H	2.992
89	1	11	CYS	HB3	H	3.139
90	1	11	CYS	CA	C	60.912
91	1	11	CYS	CB	C	25.611
92	1	12	GLY	H	H	8.189
93	1	12	GLY	HA2	H	3.954
94	1	12	GLY	HA3	H	3.954
95	1	12	GLY	CA	C	45.073
96	1	13	LEU	H	H	8.080
97	1	13	LEU	HA	H	4.295
98	1	13	LEU	HB2	H	1.651
99	1	13	LEU	HB3	H	1.839
100	1	13	LEU	HG	H	1.759
101	1	13	LEU	HD11	H	0.904
102	1	13	LEU	HD12	H	0.904
103	1	13	LEU	HD13	H	0.904
104	1	13	LEU	CA	C	55.824
105	1	13	LEU	CB	C	40.934
106	1	13	LEU	CG	C	25.839
107	1	13	LEU	CD1	C	21.548
108	1	14	ALA	H	H	8.124
109	1	14	ALA	HA	H	4.225
110	1	14	ALA	HB1	H	1.506
111	1	14	ALA	HB2	H	1.506
112	1	14	ALA	HB3	H	1.506
113	1	14	ALA	CA	C	52.999
114	1	14	ALA	CB	C	16.679
115	1	15	GLY	H	H	8.171
116	1	15	GLY	HA2	H	3.957
117	1	15	GLY	HA3	H	3.957
118	1	15	GLY	CA	C	45.073
119	1	16	GLY	H	H	8.168
120	1	16	GLY	HA2	H	4.006
121	1	16	GLY	HA3	H	4.008
122	1	16	GLY	CA	C	45.016
123	1	17	SER	H	H	8.001
124	1	17	SER	HA	H	4.285
125	1	17	SER	HB2	H	4.033
126	1	17	SER	HB3	H	3.962
127	1	17	SER	CA	C	59.763
128	1	17	SER	CB	C	62.011
129	1	18	ALA	H	H	7.905
130	1	18	ALA	HA	H	4.108
131	1	18	ALA	HB1	H	1.482
132	1	18	ALA	HB2	H	1.482
133	1	18	ALA	HB3	H	1.482
134	1	18	ALA	CA	C	53.930
135	1	18	ALA	CB	C	16.437
136	1	19	ALA	H	H	7.688
137	1	19	ALA	HA	H	4.153
138	1	19	ALA	HB1	H	1.542
139	1	19	ALA	HB2	H	1.542
140	1	19	ALA	HB3	H	1.542
141	1	19	ALA	CA	C	53.846
142	1	19	ALA	CB	C	16.716
143	1	20	VAL	H	H	7.704
144	1	20	VAL	HA	H	3.699
145	1	20	VAL	HB	H	2.237
146	1	20	VAL	HG11	H	1.087
147	1	20	VAL	HG12	H	1.087
148	1	20	VAL	HG13	H	1.087
149	1	20	VAL	HG21	H	0.974
150	1	20	VAL	HG22	H	0.974
151	1	20	VAL	HG23	H	0.974
152	1	20	VAL	CA	C	65.086
153	1	20	VAL	CB	C	30.710
154	1	20	VAL	CG1	C	20.309
155	1	20	VAL	CG2	C	19.235
156	1	21	LEU	H	H	7.809
157	1	21	LEU	HA	H	4.124
158	1	21	LEU	HB2	H	1.696
159	1	21	LEU	HB3	H	1.696
160	1	21	LEU	HG	H	1.646
161	1	21	LEU	HD11	H	0.848
162	1	21	LEU	HD12	H	0.848
163	1	21	LEU	HD13	H	0.848
164	1	21	LEU	CA	C	56.896
165	1	21	LEU	CB	C	40.545
166	1	21	LEU	CG	C	25.844
167	1	21	LEU	CD1	C	21.975
168	1	22	PHE	H	H	8.434
169	1	22	PHE	HA	H	4.349
170	1	22	PHE	HB2	H	3.210
171	1	22	PHE	HB3	H	3.210
172	1	22	PHE	CA	C	59.343
173	1	22	PHE	CB	C	37.469
174	1	23	SER	H	H	8.008
175	1	23	SER	HA	H	4.194
176	1	23	SER	HB2	H	4.032
177	1	23	SER	HB3	H	4.128
178	1	23	SER	CA	C	60.577
179	1	23	SER	CB	C	61.983
180	1	24	ALA	H	H	8.009
181	1	24	ALA	HA	H	4.182
182	1	24	ALA	HB1	H	1.539
183	1	24	ALA	HB2	H	1.539
184	1	24	ALA	HB3	H	1.539
185	1	24	ALA	CA	C	53.596
186	1	24	ALA	CB	C	16.716
187	1	25	VAL	H	H	7.888
188	1	25	VAL	HA	H	3.833
189	1	25	VAL	HB	H	2.167
190	1	25	VAL	HG11	H	0.944
191	1	25	VAL	HG12	H	0.944
192	1	25	VAL	HG13	H	0.944
193	1	25	VAL	HG21	H	1.049
194	1	25	VAL	HG22	H	1.049
195	1	25	VAL	HG23	H	1.049
196	1	25	VAL	CA	C	63.578
197	1	25	VAL	CB	C	30.956
198	1	25	VAL	CG1	C	19.399
199	1	25	VAL	CG2	C	19.815
200	1	26	ALA	H	H	7.936
201	1	26	ALA	HA	H	4.118
202	1	26	ALA	HB1	H	1.335
203	1	26	ALA	HB2	H	1.335
204	1	26	ALA	HB3	H	1.335
205	1	26	ALA	CA	C	53.020
206	1	26	ALA	CB	C	17.303
207	1	27	VAL	H	H	7.642
208	1	27	VAL	HA	H	3.986
209	1	27	VAL	HB	H	2.210
210	1	27	VAL	HG11	H	0.974
211	1	27	VAL	HG12	H	0.974
212	1	27	VAL	HG13	H	0.974
213	1	27	VAL	HG21	H	1.041
214	1	27	VAL	HG22	H	1.041
215	1	27	VAL	HG23	H	1.041
216	1	27	VAL	CA	C	62.629
217	1	27	VAL	CB	C	31.009
218	1	27	VAL	CG1	C	19.235
219	1	27	VAL	CG2	C	19.430
220	1	28	GLY	H	H	7.969
221	1	28	GLY	HA2	H	3.928
222	1	28	GLY	HA3	H	3.928
223	1	28	GLY	CA	C	44.221
224	1	29	LYS	H	H	7.654
225	1	29	LYS	HA	H	4.631
226	1	29	LYS	HB2	H	1.848
227	1	29	LYS	HB3	H	1.848
228	1	29	LYS	HG2	H	1.502
229	1	29	LYS	HG3	H	1.502
230	1	29	LYS	HD2	H	1.712
231	1	29	LYS	HD3	H	1.712
232	1	29	LYS	HE2	H	3.018
233	1	29	LYS	HE3	H	3.018
234	1	29	LYS	CA	C	53.353
235	1	29	LYS	CB	C	31.149
236	1	29	LYS	CG	C	23.123
237	1	29	LYS	CD	C	27.628
238	1	29	LYS	CE	C	41.149
239	1	30	PRO	HA	H	4.404
240	1	30	PRO	HB2	H	2.287
241	1	30	PRO	HB3	H	1.916
242	1	30	PRO	HG2	H	2.008
243	1	30	PRO	HG3	H	2.075
244	1	30	PRO	HD2	H	3.830
245	1	30	PRO	HD3	H	3.620
246	1	30	PRO	CA	C	62.418
247	1	30	PRO	CB	C	30.487
248	1	30	PRO	CG	C	25.985
249	1	30	PRO	CD	C	49.250
250	1	31	ARG	H	H	7.930
251	1	31	ARG	HA	H	4.398
252	1	31	ARG	HB2	H	1.923
253	1	31	ARG	HB3	H	1.784
254	1	31	ARG	HG2	H	1.689
255	1	31	ARG	HG3	H	1.689
256	1	31	ARG	HD2	H	3.212
257	1	31	ARG	HD3	H	3.212
258	1	31	ARG	CA	C	54.490
259	1	31	ARG	CB	C	29.866
260	1	31	ARG	CG	C	25.777
261	1	31	ARG	CD	C	42.056
262	1	32	ALA	H	H	7.929
263	1	32	ALA	HA	H	4.347
264	1	32	ALA	HB1	H	1.408
265	1	32	ALA	HB2	H	1.408
266	1	32	ALA	HB3	H	1.408
267	1	32	ALA	CA	C	51.395
268	1	32	ALA	CB	C	17.506
269	1	33	GLY	H	H	8.065
270	1	33	GLY	HA2	H	4.041
271	1	33	GLY	HA3	H	3.878
272	1	33	GLY	CA	C	44.014
273	1	34	GLY	H	H	8.004
274	1	34	GLY	HA2	H	3.954
275	1	34	GLY	HA3	H	3.954
276	1	34	GLY	CA	C	44.214
277	1	35	ASP	H	H	8.096
278	1	35	ASP	HA	H	4.653
279	1	35	ASP	HB2	H	2.702
280	1	35	ASP	HB3	H	2.702
281	1	35	ASP	CA	C	52.398
282	1	35	ASP	CB	C	39.550

Release date

2022-04-25

Citation

Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state
Siebert, V., Silber, M., Heuten, E., Muhle-Goll, C., Lemberg, M.K.
J. Biol. Chem. (2022), 298, 102321-102321, PubMed 35921890 , DOI 10.1016/j.jbc.2022.102321 , Abstract

Related entities 1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16), : 4 : 2 : 4 entities Detail

Interaction partners 1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16), : 56 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail