BMRB: 34682

Three-dimensional structure of the PGAM5 C12S mutant TMD

Authors

Silber, M., Muhle-Goll, C.

Assembly

Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16)

Entity

1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16) (polymer, Thiol state: not present), 35 monomers, 3285.710 Da Detail

AFRQALQLAA SGLAGGSAAV LFSAVAVGKP RAGGD

Formula weight

3285.71 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 188, ¹³C: 83, ¹⁵N: 34 assignments Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	ALA	HA	H	4.167
2	1	1	ALA	HB1	H	1.369
3	1	1	ALA	HB2	H	1.369
4	1	1	ALA	HB3	H	1.369
5	1	1	ALA	CA	C	52.8
6	1	1	ALA	CB	C	16.335
7	1	1	ALA	N	N	128.937
8	1	2	PHE	H	H	7.388
9	1	2	PHE	HA	H	4.442
10	1	2	PHE	HB2	H	3.128
11	1	2	PHE	HB3	H	3.18
12	1	2	PHE	HD1	H	7.177
13	1	2	PHE	HD2	H	7.177
14	1	2	PHE	HE1	H	7.176
15	1	2	PHE	HE2	H	7.176
16	1	2	PHE	CA	C	57.557
17	1	2	PHE	CB	C	37.043
18	1	2	PHE	N	N	114.359
19	1	3	ARG	H	H	7.469
20	1	3	ARG	HA	H	3.965
21	1	3	ARG	HB2	H	1.811
22	1	3	ARG	HB3	H	1.807
23	1	3	ARG	HG2	H	1.558
24	1	3	ARG	HG3	H	1.591
25	1	3	ARG	HD2	H	3.179
26	1	3	ARG	HD3	H	3.177
27	1	3	ARG	CA	C	57.783
28	1	3	ARG	CB	C	28.325
29	1	3	ARG	CG	C	25.999
30	1	3	ARG	CD	C	41.704
31	1	3	ARG	N	N	118.876
32	1	4	GLN	H	H	7.893
33	1	4	GLN	HA	H	4.117
34	1	4	GLN	HB2	H	2.152
35	1	4	GLN	HB3	H	2.152
36	1	4	GLN	HG2	H	2.382
37	1	4	GLN	HG3	H	2.382
38	1	4	GLN	CA	C	57.554
39	1	4	GLN	CB	C	27.029
40	1	4	GLN	CG	C	32.733
41	1	4	GLN	N	N	117.7
42	1	5	ALA	H	H	7.77
43	1	5	ALA	HA	H	4.106
44	1	5	ALA	HB1	H	1.513
45	1	5	ALA	HB2	H	1.513
46	1	5	ALA	HB3	H	1.513
47	1	5	ALA	CA	C	53.814
48	1	5	ALA	CB	C	16.367
49	1	5	ALA	N	N	121.548
50	1	6	LEU	H	H	7.891
51	1	6	LEU	HA	H	4.123
52	1	6	LEU	HB2	H	1.784
53	1	6	LEU	HB3	H	1.642
54	1	6	LEU	CA	C	56.606
55	1	6	LEU	CB	C	40.48
56	1	6	LEU	N	N	117.653
57	1	7	GLN	H	H	7.848
58	1	7	GLN	HA	H	4.023
59	1	7	GLN	HB2	H	2.165
60	1	7	GLN	HB3	H	2.228
61	1	7	GLN	HG2	H	2.36
62	1	7	GLN	HG3	H	2.502
63	1	7	GLN	CA	C	57.7
64	1	7	GLN	CB	C	27.039
65	1	7	GLN	CG	C	32.618
66	1	7	GLN	N	N	117.304
67	1	8	LEU	H	H	8.107
68	1	8	LEU	HA	H	4.115
69	1	8	LEU	HB2	H	1.914
70	1	8	LEU	HB3	H	1.644
71	1	8	LEU	HG	H	1.773
72	1	8	LEU	CA	C	56.773
73	1	8	LEU	CB	C	40.376
74	1	8	LEU	CG	C	25.711
75	1	8	LEU	N	N	120.811
76	1	9	ALA	H	H	8.291
77	1	9	ALA	HA	H	4.13
78	1	9	ALA	HB1	H	1.54
79	1	9	ALA	HB2	H	1.54
80	1	9	ALA	HB3	H	1.54
81	1	9	ALA	CA	C	53.812
82	1	9	ALA	CB	C	16.116
83	1	9	ALA	N	N	122.983
84	1	10	ALA	H	H	8.517
85	1	10	ALA	HA	H	4.141
86	1	10	ALA	HB1	H	1.519
87	1	10	ALA	HB2	H	1.519
88	1	10	ALA	HB3	H	1.519
89	1	10	ALA	CA	C	53.802
90	1	10	ALA	CB	C	16.359
91	1	10	ALA	N	N	119.812
92	1	11	SER	H	H	7.979
93	1	11	SER	HA	H	4.27
94	1	11	SER	HB2	H	4.062
95	1	11	SER	HB3	H	4.062
96	1	11	SER	CA	C	59.799
97	1	11	SER	CB	C	62.059
98	1	11	SER	N	N	112.297
99	1	12	GLY	H	H	8.106
100	1	12	GLY	HA2	H	3.953
101	1	12	GLY	HA3	H	3.952
102	1	12	GLY	CA	C	44.808
103	1	12	GLY	N	N	105.202
104	1	13	LEU	H	H	7.983
105	1	13	LEU	HA	H	4.276
106	1	13	LEU	HB2	H	1.632
107	1	13	LEU	HB3	H	1.824
108	1	13	LEU	HG	H	1.734
109	1	13	LEU	CA	C	55.622
110	1	13	LEU	CB	C	40.732
111	1	13	LEU	CG	C	25.656
112	1	13	LEU	N	N	121.919
113	1	14	ALA	H	H	8.103
114	1	14	ALA	HA	H	4.221
115	1	14	ALA	HB1	H	1.476
116	1	14	ALA	HB2	H	1.476
117	1	14	ALA	HB3	H	1.476
118	1	14	ALA	CA	C	52.765
119	1	14	ALA	CB	C	16.528
120	1	14	ALA	N	N	121.676
121	1	15	GLY	H	H	7.98
122	1	15	GLY	HA2	H	3.993
123	1	15	GLY	HA3	H	3.993
124	1	15	GLY	CA	C	44.813
125	1	15	GLY	N	N	109.473
126	1	16	GLY	H	H	8.121
127	1	16	GLY	HA2	H	3.956
128	1	16	GLY	HA3	H	4.004
129	1	16	GLY	CA	C	44.809
130	1	16	GLY	N	N	108.037
131	1	17	SER	H	H	7.981
132	1	17	SER	HA	H	4.28
133	1	17	SER	HB2	H	3.958
134	1	17	SER	HB3	H	4.016
135	1	17	SER	CA	C	59.568
136	1	17	SER	CB	C	61.853
137	1	17	SER	N	N	115.116
138	1	18	ALA	H	H	7.909
139	1	18	ALA	HA	H	4.101
140	1	18	ALA	HB1	H	1.472
141	1	18	ALA	HB2	H	1.472
142	1	18	ALA	HB3	H	1.472
143	1	18	ALA	CA	C	53.71
144	1	18	ALA	CB	C	16.272
145	1	18	ALA	N	N	123.995
146	1	19	ALA	H	H	7.681
147	1	19	ALA	HA	H	4.143
148	1	19	ALA	HB1	H	1.527
149	1	19	ALA	HB2	H	1.527
150	1	19	ALA	HB3	H	1.527
151	1	19	ALA	CA	C	53.384
152	1	19	ALA	CB	C	16.115
153	1	19	ALA	N	N	118.301
154	1	20	VAL	H	H	7.678
155	1	20	VAL	HA	H	3.694
156	1	20	VAL	HB	H	2.229
157	1	20	VAL	HG11	H	1.073
158	1	20	VAL	HG12	H	1.073
159	1	20	VAL	HG13	H	1.073
160	1	20	VAL	HG21	H	0.96
161	1	20	VAL	HG22	H	0.96
162	1	20	VAL	HG23	H	0.96
163	1	20	VAL	CA	C	64.9
164	1	20	VAL	CB	C	30.556
165	1	20	VAL	CG1	C	20.133
166	1	20	VAL	CG2	C	19.068
167	1	20	VAL	N	N	118.301
168	1	21	LEU	H	H	7.773
169	1	21	LEU	HA	H	4.11
170	1	21	LEU	HB2	H	1.631
171	1	21	LEU	HB3	H	1.687
172	1	21	LEU	CA	C	56.773
173	1	21	LEU	CB	C	40.395
174	1	21	LEU	N	N	120.58
175	1	22	PHE	H	H	8.397
176	1	22	PHE	HA	H	4.344
177	1	22	PHE	HB3	H	3.2
178	1	22	PHE	HD1	H	7.21
179	1	22	PHE	HD2	H	7.21
180	1	22	PHE	HE1	H	7.21
181	1	22	PHE	HE2	H	7.21
182	1	22	PHE	CA	C	59.171
183	1	22	PHE	CB	C	37.307
184	1	22	PHE	N	N	116.608
185	1	23	SER	H	H	7.988
186	1	23	SER	HA	H	4.186
187	1	23	SER	HB2	H	4.027
188	1	23	SER	HB3	H	4.122
189	1	23	SER	CA	C	60.4
190	1	23	SER	CB	C	61.82
191	1	23	SER	N	N	113.863
192	1	24	ALA	H	H	7.991
193	1	24	ALA	HA	H	4.174
194	1	24	ALA	HB1	H	1.531
195	1	24	ALA	HB2	H	1.531
196	1	24	ALA	HB3	H	1.531
197	1	24	ALA	CA	C	53.41
198	1	24	ALA	CB	C	16.631
199	1	24	ALA	N	N	123.856
200	1	25	VAL	H	H	7.865
201	1	25	VAL	HA	H	3.83
202	1	25	VAL	HB	H	2.164
203	1	25	VAL	HG11	H	0.937
204	1	25	VAL	HG12	H	0.937
205	1	25	VAL	HG13	H	0.937
206	1	25	VAL	HG21	H	1.037
207	1	25	VAL	HG22	H	1.037
208	1	25	VAL	HG23	H	1.037
209	1	25	VAL	CA	C	63.401
210	1	25	VAL	CB	C	30.834
211	1	25	VAL	CG1	C	19.227
212	1	25	VAL	CG2	C	19.63
213	1	25	VAL	N	N	115.912
214	1	26	ALA	H	H	7.922
215	1	26	ALA	HA	H	4.117
216	1	26	ALA	HB1	H	1.327
217	1	26	ALA	HB2	H	1.327
218	1	26	ALA	HB3	H	1.327
219	1	26	ALA	CA	C	52.864
220	1	26	ALA	CB	C	17.144
221	1	26	ALA	N	N	121.849
222	1	27	VAL	H	H	7.621
223	1	27	VAL	HA	H	3.986
224	1	27	VAL	HB	H	2.203
225	1	27	VAL	HG11	H	1.028
226	1	27	VAL	HG12	H	1.028
227	1	27	VAL	HG13	H	1.028
228	1	27	VAL	HG21	H	0.965
229	1	27	VAL	HG22	H	0.965
230	1	27	VAL	HG23	H	0.965
231	1	27	VAL	CA	C	62.45
232	1	27	VAL	CB	C	30.86
233	1	27	VAL	CG1	C	19.276
234	1	27	VAL	CG2	C	19.068
235	1	27	VAL	N	N	114.666
236	1	28	GLY	H	H	7.958
237	1	28	GLY	HA2	H	3.92
238	1	28	GLY	HA3	H	3.92
239	1	28	GLY	CA	C	44.045
240	1	28	GLY	N	N	109
241	1	29	LYS	H	H	7.65
242	1	29	LYS	HA	H	4.626
243	1	29	LYS	HB2	H	1.843
244	1	29	LYS	HB3	H	1.843
245	1	29	LYS	HG2	H	1.49
246	1	29	LYS	HG3	H	1.49
247	1	29	LYS	HD2	H	1.706
248	1	29	LYS	HD3	H	1.706
249	1	29	LYS	HE2	H	3.007
250	1	29	LYS	HE3	H	3.007
251	1	29	LYS	CA	C	53.17
252	1	29	LYS	CB	C	31.004
253	1	29	LYS	CG	C	22.955
254	1	29	LYS	CD	C	27.479
255	1	29	LYS	CE	C	40.978
256	1	29	LYS	N	N	120.209
257	1	30	PRO	HA	H	4.405
258	1	30	PRO	HB2	H	1.903
259	1	30	PRO	HB3	H	2.278
260	1	30	PRO	HG2	H	1.998
261	1	30	PRO	HG3	H	2.068
262	1	30	PRO	HD2	H	3.819
263	1	30	PRO	HD3	H	3.612
264	1	30	PRO	CA	C	62.247
265	1	30	PRO	CB	C	30.323
266	1	30	PRO	CG	C	25.831
267	1	30	PRO	CD	C	49.088
268	1	31	ARG	H	H	7.903
269	1	31	ARG	HA	H	4.396
270	1	31	ARG	HB2	H	1.784
271	1	31	ARG	HB3	H	1.916
272	1	31	ARG	HG2	H	1.685
273	1	31	ARG	HG3	H	1.685
274	1	31	ARG	HD2	H	3.212
275	1	31	ARG	HD3	H	3.204
276	1	31	ARG	CA	C	54.321
277	1	31	ARG	CB	C	29.719
278	1	31	ARG	CG	C	25.613
279	1	31	ARG	CD	C	41.913
280	1	31	ARG	N	N	119.339
281	1	32	ALA	H	H	7.939
282	1	32	ALA	HA	H	4.341
283	1	32	ALA	HB1	H	1.402
284	1	32	ALA	HB2	H	1.402
285	1	32	ALA	HB3	H	1.402
286	1	32	ALA	CA	C	51.241
287	1	32	ALA	CB	C	17.368
288	1	32	ALA	N	N	123.995
289	1	33	GLY	H	H	8.066
290	1	33	GLY	HA2	H	3.879
291	1	33	GLY	HA3	H	4.027
292	1	33	GLY	CA	C	43.855
293	1	33	GLY	N	N	106.732
294	1	34	GLY	H	H	8.053
295	1	34	GLY	HA2	H	3.939
296	1	34	GLY	HA3	H	3.939
297	1	34	GLY	CA	C	44.045
298	1	34	GLY	N	N	107.606
299	1	35	ASP	H	H	8.108
300	1	35	ASP	HA	H	4.638
301	1	35	ASP	HB2	H	2.686
302	1	35	ASP	HB3	H	2.686
303	1	35	ASP	CA	C	52.324
304	1	35	ASP	CB	C	39.566
305	1	35	ASP	N	N	119.258

Release date

2022-04-25

Citation

Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state
Siebert, V., Silber, M., Heuten, E., Muhle-Goll, C., Lemberg, M.K.
J. Biol. Chem. (2022), 298, 102321-102321, PubMed 35921890 , DOI 10.1016/j.jbc.2022.102321 , Abstract

Related entities 1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16), : 6 entities Detail

Interaction partners 1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16), : 56 interactors Detail

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail