BMRB: 34683

Three-dimensional structure of the PGAM5 G17L mutant TMD

Authors

Silber, M., Muhle-Goll, C.

Assembly

Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16)

Entity

1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16) (polymer), 35 monomers, 3357.881 Da Detail

AFRQALQLAA CGLAGLSAAV LFSAVAVGKP RAGGD

Formula weight

3357.881 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 188, ¹³C: 82 assignments Detail

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	ALA	HA	H	4.167
2	1	1	ALA	HB1	H	1.374
3	1	1	ALA	HB2	H	1.374
4	1	1	ALA	HB3	H	1.374
5	1	1	ALA	CA	C	52.855
6	1	1	ALA	CB	C	16.357
7	1	2	PHE	H	H	7.351
8	1	2	PHE	HA	H	4.448
9	1	2	PHE	HB2	H	3.13
10	1	2	PHE	HB3	H	3.184
11	1	2	PHE	CA	C	57.51
12	1	2	PHE	CB	C	37.074
13	1	3	ARG	H	H	7.436
14	1	3	ARG	HA	H	3.972
15	1	3	ARG	HB2	H	1.792
16	1	3	ARG	HB3	H	1.834
17	1	3	ARG	HG2	H	1.549
18	1	3	ARG	HG3	H	1.592
19	1	3	ARG	HD2	H	3.175
20	1	3	ARG	HD3	H	3.175
21	1	3	ARG	CA	C	57.769
22	1	3	ARG	CB	C	28.357
23	1	3	ARG	CG	C	26.041
24	1	3	ARG	CD	C	41.764
25	1	4	GLN	H	H	7.894
26	1	4	GLN	HA	H	4.127
27	1	4	GLN	HB2	H	2.155
28	1	4	GLN	HB3	H	2.155
29	1	4	GLN	HG2	H	2.388
30	1	4	GLN	HG3	H	2.388
31	1	4	GLN	CA	C	57.564
32	1	4	GLN	CB	C	27.088
33	1	4	GLN	CG	C	32.75
34	1	5	ALA	H	H	7.767
35	1	5	ALA	HA	H	4.109
36	1	5	ALA	HB1	H	1.523
37	1	5	ALA	HB2	H	1.523
38	1	5	ALA	HB3	H	1.523
39	1	5	ALA	CA	C	53.912
40	1	5	ALA	CB	C	16.196
41	1	6	LEU	H	H	7.867
42	1	6	LEU	HA	H	4.133
43	1	6	LEU	HB2	H	1.632
44	1	6	LEU	HB3	H	1.806
45	1	6	LEU	CA	C	57.034
46	1	6	LEU	CB	C	40.529
47	1	7	GLN	H	H	7.835
48	1	7	GLN	HA	H	4.073
49	1	7	GLN	HB2	H	2.234
50	1	7	GLN	HB3	H	2.205
51	1	7	GLN	HG2	H	2.384
52	1	7	GLN	HG3	H	2.504
53	1	7	GLN	CA	C	57.677
54	1	7	GLN	CB	C	27.215
55	1	7	GLN	CG	C	32.73
56	1	8	LEU	H	H	8.037
57	1	8	LEU	HA	H	4.142
58	1	8	LEU	HB2	H	1.628
59	1	8	LEU	HB3	H	1.911
60	1	8	LEU	CA	C	56.557
61	1	8	LEU	CB	C	40.469
62	1	9	ALA	H	H	8.162
63	1	9	ALA	HA	H	4.137
64	1	9	ALA	HB1	H	1.549
65	1	9	ALA	HB2	H	1.549
66	1	9	ALA	HB3	H	1.549
67	1	9	ALA	CA	C	53.842
68	1	9	ALA	CB	C	16.221
69	1	10	ALA	H	H	8.303
70	1	10	ALA	HA	H	4.138
71	1	10	ALA	HB1	H	1.566
72	1	10	ALA	HB2	H	1.566
73	1	10	ALA	HB3	H	1.566
74	1	10	ALA	CA	C	53.842
75	1	10	ALA	CB	C	16.281
76	1	11	CYS	H	H	8.116
77	1	11	CYS	HA	H	4.285
78	1	11	CYS	HB2	H	3.145
79	1	11	CYS	HB3	H	2.989
80	1	11	CYS	CA	C	61.056
81	1	11	CYS	CB	C	25.416
82	1	12	GLY	H	H	8.235
83	1	12	GLY	HA2	H	3.953
84	1	12	GLY	HA3	H	3.951
85	1	12	GLY	CA	C	45.268
86	1	13	LEU	H	H	8.179
87	1	13	LEU	HA	H	4.272
88	1	13	LEU	HB2	H	1.69
89	1	13	LEU	HB3	H	1.827
90	1	13	LEU	CA	C	56.38
91	1	13	LEU	CB	C	40.529
92	1	14	ALA	H	H	8.217
93	1	14	ALA	HA	H	4.134
94	1	14	ALA	HB1	H	1.543
95	1	14	ALA	HB2	H	1.543
96	1	14	ALA	HB3	H	1.543
97	1	14	ALA	CA	C	53.842
98	1	14	ALA	CB	C	16.677
99	1	15	GLY	H	H	8.16
100	1	15	GLY	HA2	H	3.954
101	1	15	GLY	HA3	H	3.955
102	1	15	GLY	CA	C	45.268
103	1	16	LEU	H	H	8.056
104	1	16	LEU	HA	H	4.271
105	1	16	LEU	HB2	H	1.802
106	1	16	LEU	HB3	H	1.771
107	1	16	LEU	HD11	H	0.945
108	1	16	LEU	HD12	H	0.945
109	1	16	LEU	HD13	H	0.945
110	1	16	LEU	HD21	H	0.908
111	1	16	LEU	HD22	H	0.908
112	1	16	LEU	HD23	H	0.908
113	1	16	LEU	CA	C	56.171
114	1	16	LEU	CB	C	40.858
115	1	17	SER	H	H	8.159
116	1	17	SER	HA	H	4.132
117	1	17	SER	HB2	H	4.081
118	1	17	SER	HB3	H	4.081
119	1	17	SER	CA	C	60.708
120	1	17	SER	CB	C	61.68
121	1	18	ALA	H	H	7.788
122	1	18	ALA	HA	H	4.128
123	1	18	ALA	HB1	H	1.523
124	1	18	ALA	HB2	H	1.523
125	1	18	ALA	HB3	H	1.523
126	1	18	ALA	CA	C	53.842
127	1	18	ALA	CB	C	16.196
128	1	19	ALA	H	H	7.793
129	1	19	ALA	HA	H	4.174
130	1	19	ALA	HB1	H	1.593
131	1	19	ALA	HB2	H	1.593
132	1	19	ALA	HB3	H	1.593
133	1	19	ALA	CA	C	53.519
134	1	19	ALA	CB	C	16.245
135	1	20	VAL	H	H	8.275
136	1	20	VAL	HA	H	3.66
137	1	20	VAL	HB	H	2.234
138	1	20	VAL	HG11	H	1.092
139	1	20	VAL	HG12	H	1.092
140	1	20	VAL	HG13	H	1.092
141	1	20	VAL	HG21	H	0.956
142	1	20	VAL	HG22	H	0.956
143	1	20	VAL	HG23	H	0.956
144	1	20	VAL	CA	C	65.459
145	1	20	VAL	CB	C	30.558
146	1	20	VAL	CG1	C	20.483
147	1	20	VAL	CG2	C	19.154
148	1	21	LEU	H	H	8.03
149	1	21	LEU	HA	H	4.13
150	1	21	LEU	HB2	H	1.679
151	1	21	LEU	HB3	H	1.742
152	1	21	LEU	HD11	H	0.855
153	1	21	LEU	HD12	H	0.855
154	1	21	LEU	HD13	H	0.855
155	1	21	LEU	CA	C	56.557
156	1	21	LEU	CB	C	40.501
157	1	22	PHE	H	H	8.558
158	1	22	PHE	HA	H	4.321
159	1	22	PHE	HB2	H	3.234
160	1	22	PHE	HB3	H	3.234
161	1	22	PHE	CA	C	59.533
162	1	22	PHE	CB	C	37.414
163	1	23	SER	H	H	8.108
164	1	23	SER	HA	H	4.172
165	1	23	SER	HB2	H	4.136
166	1	23	SER	HB3	H	4.035
167	1	23	SER	CA	C	60.587
168	1	23	SER	CB	C	61.78
169	1	24	ALA	H	H	8.059
170	1	24	ALA	HA	H	4.173
171	1	24	ALA	HB1	H	1.545
172	1	24	ALA	HB2	H	1.545
173	1	24	ALA	HB3	H	1.545
174	1	24	ALA	CA	C	53.519
175	1	24	ALA	CB	C	16.677
176	1	25	VAL	H	H	7.928
177	1	25	VAL	HA	H	3.827
178	1	25	VAL	HB	H	2.164
179	1	25	VAL	HG11	H	1.043
180	1	25	VAL	HG12	H	1.043
181	1	25	VAL	HG13	H	1.043
182	1	25	VAL	HG21	H	0.929
183	1	25	VAL	HG22	H	0.929
184	1	25	VAL	HG23	H	0.929
185	1	25	VAL	CA	C	63.532
186	1	25	VAL	CB	C	30.858
187	1	25	VAL	CG1	C	19.75
188	1	25	VAL	CG2	C	19.247
189	1	26	ALA	H	H	7.973
190	1	26	ALA	HA	H	4.102
191	1	26	ALA	HB1	H	1.304
192	1	26	ALA	HB2	H	1.304
193	1	26	ALA	HB3	H	1.304
194	1	26	ALA	CA	C	52.952
195	1	26	ALA	CB	C	17.161
196	1	27	VAL	H	H	7.623
197	1	27	VAL	HA	H	3.985
198	1	27	VAL	HB	H	2.208
199	1	27	VAL	HG11	H	1.033
200	1	27	VAL	HG12	H	1.033
201	1	27	VAL	HG13	H	1.033
202	1	27	VAL	HG21	H	0.971
203	1	27	VAL	HG22	H	0.971
204	1	27	VAL	HG23	H	0.971
205	1	27	VAL	CA	C	62.521
206	1	27	VAL	CB	C	30.88
207	1	27	VAL	CG1	C	19.339
208	1	27	VAL	CG2	C	19.154
209	1	28	GLY	H	H	7.96
210	1	28	GLY	HA2	H	3.919
211	1	28	GLY	HA3	H	3.919
212	1	28	GLY	CA	C	44.099
213	1	29	LYS	H	H	7.658
214	1	29	LYS	HA	H	4.628
215	1	29	LYS	HB2	H	1.847
216	1	29	LYS	HB3	H	1.847
217	1	29	LYS	HG2	H	1.495
218	1	29	LYS	HG3	H	1.497
219	1	29	LYS	HD2	H	1.708
220	1	29	LYS	HD3	H	1.708
221	1	29	LYS	HE2	H	3.01
222	1	29	LYS	HE3	H	3.01
223	1	29	LYS	CA	C	53.241
224	1	29	LYS	CB	C	31.014
225	1	29	LYS	CG	C	22.992
226	1	29	LYS	CD	C	27.508
227	1	29	LYS	CE	C	41.015
228	1	30	PRO	HA	H	4.404
229	1	30	PRO	HB2	H	2.281
230	1	30	PRO	HB3	H	1.906
231	1	30	PRO	HG2	H	1.998
232	1	30	PRO	HG3	H	2.073
233	1	30	PRO	HD2	H	3.617
234	1	30	PRO	HD3	H	3.826
235	1	30	PRO	CA	C	62.3
236	1	30	PRO	CB	C	30.367
237	1	30	PRO	CG	C	25.868
238	1	30	PRO	CD	C	49.131
239	1	31	ARG	H	H	7.897
240	1	31	ARG	HA	H	4.394
241	1	31	ARG	HB2	H	1.782
242	1	31	ARG	HB3	H	1.919
243	1	31	ARG	HG2	H	1.687
244	1	31	ARG	HD2	H	3.204
245	1	31	ARG	HD3	H	3.204
246	1	31	ARG	CA	C	54.347
247	1	31	ARG	CB	C	29.742
248	1	31	ARG	CG	C	25.671
249	1	31	ARG	CD	C	41.926
250	1	32	ALA	H	H	7.934
251	1	32	ALA	HA	H	4.344
252	1	32	ALA	HB1	H	1.405
253	1	32	ALA	HB2	H	1.405
254	1	32	ALA	HB3	H	1.405
255	1	32	ALA	CA	C	51.267
256	1	32	ALA	CB	C	17.399
257	1	33	GLY	H	H	8.066
258	1	33	GLY	HA2	H	4.033
259	1	33	GLY	HA3	H	3.884
260	1	33	GLY	CA	C	43.905
261	1	34	GLY	H	H	8.052
262	1	34	GLY	HA2	H	3.943
263	1	34	GLY	HA3	H	3.943
264	1	34	GLY	CA	C	44.083
265	1	35	ASP	H	H	8.114
266	1	35	ASP	HA	H	4.638
267	1	35	ASP	HB2	H	2.686
268	1	35	ASP	HB3	H	2.686
269	1	35	ASP	CA	C	52.423
270	1	35	ASP	CB	C	39.663

Release date

2022-04-25

Citation

Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state
Siebert, V., Silber, M., Heuten, E., Muhle-Goll, C., Lemberg, M.K.
J. Biol. Chem. (2022), 298, 102321-102321, PubMed 35921890 , DOI 10.1016/j.jbc.2022.102321 , Abstract

Related entities 1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16), : 6 : 6 entities Detail

Interaction partners 1. Serine/threonine-protein phosphatase PGAM5, mitochondrial (E.C.3.1.3.16), : 56 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail