Refined structure of the T193A mutant in the C-terminal domain of DNAJB6b
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 31.4 % (214 of 681) | 16.1 % (58 of 360) | 41.7 % (105 of 252) | 73.9 % (51 of 69) |
Backbone | 57.3 % (196 of 342) | 37.0 % (44 of 119) | 60.8 % (101 of 166) | 89.5 % (51 of 57) |
Sidechain | 16.9 % (66 of 391) | 5.8 % (14 of 241) | 37.7 % (52 of 138) | 0.0 % (0 of 12) |
Aromatic | 0.0 % (0 of 10) | 0.0 % (0 of 5) | 0.0 % (0 of 5) | |
Methyl | 16.1 % (10 of 62) | 16.1 % (5 of 31) | 16.1 % (5 of 31) |
1. entity 1
MGNFKSISAS TKMVNGRKIT TKRIVENGQE RVEVEEDGQL KSLTINGKEQ LLRLDNKSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288 K, pH 6.7, Details 0.2 mM [U-13C; U-15N] DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN, 20 mM sodium phosphate, 150 mM sodium chloride, 0.02 % v/v sodium azide, 95% H2O/5% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNAJ HOMOLOG SUBFAMILY B MEMBER 6 C-TERMINAL DOMAIN | [U-13C; U-15N] | 0.2 mM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 150 mM | |
4 | sodium azide | natural abundance | 0.02 % v/v |