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BMRB: 34696

7QJF

Llp mutant C1G, lytic conversion lipoprotein of phage T5

Authors

Degroux, S., Mestdach, E., Vives, C., Le Roy, A., Salmon, L., Herrman, T., Breyton, C.

Assembly

Lytic conversion lipoprotein

Entity

1. Lytic conversion lipoprotein (polymer), 62 monomers, 7045.932 Da Detail

GSTFGPKDIK CEAYYMQDHV KYKANVFDRK GDMFLVSPIM AYGSFWAPVS YFTEGNTCEG VF

Formula weight

7045.932 Da

Source organism

Escherichia phage T5

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.8 %, Completeness: 62.1 %, Completeness (bb): 75.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	62.1 % (455 of 733)	64.5 % (243 of 377)	54.1 % (158 of 292)	84.4 % (54 of 64)
Backbone	75.4 % (276 of 366)	81.9 % (104 of 127)	66.1 % (119 of 180)	89.8 % (53 of 59)
Sidechain	55.3 % (234 of 423)	55.6 % (139 of 250)	56.0 % (94 of 168)	20.0 % (1 of 5)
Aromatic	22.4 % (26 of 116)	27.6 % (16 of 58)	17.5 % (10 of 57)	0.0 % (0 of 1)
Methyl	52.2 % (24 of 46)	43.5 % (10 of 23)	60.9 % (14 of 23)

1. entity 1

GSTFGPKDIK CEAYYMQDHV KYKANVFDRK GDMFLVSPIM AYGSFWAPVS YFTEGNTCEG VF

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

Release date

2022-01-10

Citation

Llp mutant C1G, lytic conversion lipoprotein of phage T5
Degroux, S., Mestdach, E., Vives, C., Le Roy, A., Salmon, L., Herrman, T., Breyton, C.

Related entities 1. Lytic conversion lipoprotein, : 1 : 2 entities Detail

Experiments performed 13 experiments Detail

1 3D 1H-15N NOESY aliphatic

Instrument

Bruker AVANCE - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

2 3D 1H-13C TOCSY

Instrument

Bruker AVANCE - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

3 3D HNCO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

4 3D HN(CA)CO

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

5 3D HNCA

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

6 3D HN(CO)CA

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

7 3D HNCACB

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

8 3D HN(COCA)CB

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

9 HAHBNH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

10 HAHB(CO)NH

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

11 2D 15N-HSQC

Instrument

Bruker AVANCE - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

12 3D 1H-13C NOESY aromatic

Instrument

Bruker AVANCE - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

13 3D 1H-15N-13C NOESY

Instrument

Bruker AVANCE - na MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.5, Details 25 mM no Tris, 75 mM no NaCl, 1.024 mM 13C 15N Sol-Llp, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Tris	natural abundance		25 (±0.2) mM
2	NaCl	natural abundance		75 (±0.2) mM
3	Sol-Llp	[U-13C; U-15N]		1.024 (±0.02) mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34696_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34696_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	502		95.2 (chain: 1, length: 62)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 62, Sequence coverage: 95.2%
- Total number of assignments
  - ¹H chemical shifts: 296
  - ¹³C chemical shifts: 151
  - ¹⁵N chemical shifts: 55
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Keywords Periplasmic protein, Phage protein, Soluble of acylated WT protein STRUCTURE FROM UNIO, UNIO VERSION 2.9.5, VIRAL PROTEIN