BMRBj - BMREntryMaster

Found 1 Document (0.728 seconds)

BMRB: 34702

7QUU

Red1-Iss10 complex

Authors

Mackereth, C.D., Kadlec, J., Laroussi, H.

Assembly

Uncharacterized protein C7D4.14c, NURS complex subunit red1

Entity

1. Uncharacterized protein C7D4.14c, NURS complex subunit red1, entity 1 (polymer, Thiol state: not present), 55 monomers, 6580.398 Da Detail

MGWSHPQFEK SSDAVEPSVE KEYKKIISFR DTVFEGKHQQ FLVPNNVRLK FLRDR

2. Uncharacterized protein C7D4.14c, NURS complex subunit red1, entity 2 (polymer, Thiol state: not present), 49 monomers, 5294.939 Da Detail

GAMGTTNQKE AEKAVSQLFE VGVRFNDFIA EGIEPSVVHT LFLKLGLDS

Total weight

11875.337 Da

Max. entity weight

6580.398 Da

Source organism

Schizosaccharomyces pombe

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.5 %, Completeness: 79.1 %, Completeness (bb): 82.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.1 % (997 of 1260)	81.3 % (533 of 656)	76.7 % (375 of 489)	77.4 % (89 of 115)
Backbone	82.6 % (509 of 616)	82.5 % (174 of 211)	82.6 % (252 of 305)	83.0 % (83 of 100)
Sidechain	77.1 % (571 of 741)	80.9 % (360 of 445)	73.0 % (205 of 281)	40.0 % (6 of 15)
Aromatic	45.9 % (56 of 122)	70.5 % (43 of 61)	21.7 % (13 of 60)	0.0 % (0 of 1)
Methyl	95.3 % (101 of 106)	94.3 % (50 of 53)	96.2 % (51 of 53)

1. entity 1

MGWSHPQFEK SSDAVEPSVE KEYKKIISFR DTVFEGKHQQ FLVPNNVRLK FLRDR

2. entity 2

GAMGTTNQKE AEKAVSQLFE VGVRFNDFIA EGIEPSVVHT LFLKLGLDS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 150 uM [U-99% 15N] Red1, 150 uM [U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Red1	[U-99% 15N]	150 uM
2	Iss10	[U-99% 15N]	150 uM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	150 mM
5	beta-mercaptoethanol	natural abundance	5 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 500 uM [U-99% 13C; U-99% 15N] Red1, 500 uM [U-99% 13C; U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 500 uM [U-99% 13C; U-99% 15N] Red1, 500 uM [U-99% 13C; U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
11	Red1	[U-99% 13C; U-99% 15N]	500 uM
12	Iss10	[U-99% 13C; U-99% 15N]	500 uM
13	TRIS	natural abundance	20 mM
14	sodium chloride	natural abundance	150 mM
15	beta-mercaptoethanol	natural abundance	5 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 150 uM [U-99% 13C; U-99% 15N] Red1, 150 uM [U-99% 13C; U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
16	Red1	[U-99% 13C; U-99% 15N]	150 uM
17	Iss10	[U-99% 13C; U-99% 15N]	150 uM
18	TRIS	natural abundance	20 mM
19	sodium chloride	natural abundance	150 mM
20	beta-mercaptoethanol	natural abundance	5 mM

Release date

2022-02-20

Citation

Structural analysis of Red1 as a conserved scaffold of the RNA-targeting MTREC/PAXT complex
Foucher, A.E., Touat-Todeschini, L., Juarez-Martinez, A.B., Rakitch, A., Laroussi, H., Karczewski, C., Acajjaoui, S., Soler-Lopez, M., Cusack, S., Mackereth, C.D., Verdel, A., Kadlec, J.
Nat. Commun. (2022), 13, 4969-4969, PubMed 36002457 , DOI 10.1038/s41467-022-32542-3 , Abstract

Related entities 1. Uncharacterized protein C7D4.14c, NURS complex subunit red1, entity 1, : 1 : 3 entities Detail

Related entities 2. Uncharacterized protein C7D4.14c, NURS complex subunit red1, entity 2, : 1 : 2 : 8 entities Detail

Interaction partners 2. Uncharacterized protein C7D4.14c, NURS complex subunit red1, entity 2, : 13 interactors Detail

More details for 13 interactors identified by 4 citations

Experiments performed 20 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 150 uM [U-99% 15N] Red1, 150 uM [U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Red1	[U-99% 15N]	150 uM
2	Iss10	[U-99% 15N]	150 uM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	150 mM
5	beta-mercaptoethanol	natural abundance	5 mM

2 2D 1H-13C HSQC

Instrument

Bruker AVANCE NEO - 800 MHz TCI 800S6 H-C/N-D-05 Z P

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 500 uM [U-99% 13C; U-99% 15N] Red1, 500 uM [U-99% 13C; U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
11	Red1	[U-99% 13C; U-99% 15N]	500 uM
12	Iss10	[U-99% 13C; U-99% 15N]	500 uM
13	TRIS	natural abundance	20 mM
14	sodium chloride	natural abundance	150 mM
15	beta-mercaptoethanol	natural abundance	5 mM

3 3D HNCO

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 500 uM [U-99% 13C; U-99% 15N] Red1, 500 uM [U-99% 13C; U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

4 3D HN(CA)CO

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

5 3D HNCA

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

6 3D HNCACB

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

7 3D CBCA(CO)NH

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

8 3D HNHA

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
1	Red1	[U-99% 15N]	150 uM
2	Iss10	[U-99% 15N]	150 uM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	150 mM
5	beta-mercaptoethanol	natural abundance	5 mM

9 3D HA(CACO)NH

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

10 3D H(CCO)NH

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

11 3D H(CCO)NH

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

12 3D HCCH-TOCSY

Instrument

Bruker AVANCE NEO - 800 MHz TCI 800S6 H-C/N-D-05 Z P

Sample

#	Name	Isotope labeling	Concentration
11	Red1	[U-99% 13C; U-99% 15N]	500 uM
12	Iss10	[U-99% 13C; U-99% 15N]	500 uM
13	TRIS	natural abundance	20 mM
14	sodium chloride	natural abundance	150 mM
15	beta-mercaptoethanol	natural abundance	5 mM

13 3D HCCH-TOCSY

Instrument

Bruker AVANCE NEO - 800 MHz TCI 800S6 H-C/N-D-05 Z P

Sample

#	Name	Isotope labeling	Concentration
11	Red1	[U-99% 13C; U-99% 15N]	500 uM
12	Iss10	[U-99% 13C; U-99% 15N]	500 uM
13	TRIS	natural abundance	20 mM
14	sodium chloride	natural abundance	150 mM
15	beta-mercaptoethanol	natural abundance	5 mM

14 3D 1H-15N NOESY

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

15 3D 1H-13C NOESY

Instrument

Bruker AVANCE NEO - 800 MHz TCI 800S6 H-C/N-D-05 Z P

Sample

#	Name	Isotope labeling	Concentration
6	Red1	[U-99% 13C; U-99% 15N]	500 uM
7	Iss10	[U-99% 13C; U-99% 15N]	500 uM
8	TRIS	natural abundance	20 mM
9	sodium chloride	natural abundance	150 mM
10	beta-mercaptoethanol	natural abundance	5 mM

16 2D 1H-1H TOCSY

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 150 uM [U-99% 13C; U-99% 15N] Red1, 150 uM [U-99% 13C; U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 100% D2O

#	Name	Isotope labeling	Concentration
16	Red1	[U-99% 13C; U-99% 15N]	150 uM
17	Iss10	[U-99% 13C; U-99% 15N]	150 uM
18	TRIS	natural abundance	20 mM
19	sodium chloride	natural abundance	150 mM
20	beta-mercaptoethanol	natural abundance	5 mM

17 2D DQF-COSY

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
16	Red1	[U-99% 13C; U-99% 15N]	150 uM
17	Iss10	[U-99% 13C; U-99% 15N]	150 uM
18	TRIS	natural abundance	20 mM
19	sodium chloride	natural abundance	150 mM
20	beta-mercaptoethanol	natural abundance	5 mM

18 2D 1H-1H NOESY

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
16	Red1	[U-99% 13C; U-99% 15N]	150 uM
17	Iss10	[U-99% 13C; U-99% 15N]	150 uM
18	TRIS	natural abundance	20 mM
19	sodium chloride	natural abundance	150 mM
20	beta-mercaptoethanol	natural abundance	5 mM

19 2D TROSY-RDC isotropic

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

#	Name	Isotope labeling	Concentration
1	Red1	[U-99% 15N]	150 uM
2	Iss10	[U-99% 15N]	150 uM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	150 mM
5	beta-mercaptoethanol	natural abundance	5 mM

20 2D TROSY-RDC phage

Instrument

Bruker AVANCE NEO - 700 MHz PA TXI 700S4 H-C/N-D-05 Z BTO

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 150 uM [U-99% 15N] Red1, 150 uM [U-99% 15N] Iss10, 20 mM TRIS, 150 mM sodium chloride, 5 mM beta-mercaptoethanol, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	Red1	[U-99% 15N]	150 uM
2	Iss10	[U-99% 15N]	150 uM
3	TRIS	natural abundance	20 mM
4	sodium chloride	natural abundance	150 mM
5	beta-mercaptoethanol	natural abundance	5 mM

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34702_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34702_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	1117		93.9 (chain: 2, length: 49), 78.2 (chain: 1, length: 55)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 2, Chain length: 49, Sequence coverage: 93.9%
  - Entity_assembly_ID: 1, Chain length: 55, Sequence coverage: 78.2%
- Total number of assignments
  - ¹H chemical shifts: 650
  - ¹³C chemical shifts: 379
  - ¹⁵N chemical shifts: 88
- Completeness of assignments
  - Entity_assemble_ID: 2
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 2
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	286	265	92.7
¹³C chemical shifts	220	184	83.6
¹⁵N chemical shifts	53	48	90.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	102	94	92.2
¹³C chemical shifts	98	88	89.8
¹⁵N chemical shifts	48	44	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	184	171	92.9
¹³C chemical shifts	122	96	78.7
¹⁵N chemical shifts	5	4	80.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	32	30	93.8
¹³C chemical shifts	32	30	93.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	22	17	77.3
¹³C chemical shifts	22	2	9.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	370	281	75.9
¹³C chemical shifts	269	195	72.5
¹⁵N chemical shifts	62	40	64.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	109	82	75.2
¹³C chemical shifts	110	83	75.5
¹⁵N chemical shifts	52	38	73.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	261	199	76.2
¹³C chemical shifts	159	112	70.4
¹⁵N chemical shifts	10	2	20.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	23	22	95.7
¹³C chemical shifts	23	22	95.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	39	26	66.7
¹³C chemical shifts	38	11	28.9
¹⁵N chemical shifts	1	0	0.0

Keywords MTREC complex, RNA, RNA degradation, meiosis

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.728 seconds)

BMRB: 34702

Sample #1

Sample #2

Sample #3

Sample #4

Related entities 1. Uncharacterized protein C7D4.14c, NURS complex subunit red1, entity 1, : 1 : 3 entities Detail

Related entities 2. Uncharacterized protein C7D4.14c, NURS complex subunit red1, entity 2, : 1 : 2 : 8 entities Detail

Interaction partners 2. Uncharacterized protein C7D4.14c, NURS complex subunit red1, entity 2, : 13 interactors Detail

Experiments performed 20 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail