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BMRB: 34705

7QZV

Hm-AMP2

Authors

Grafskaia, E.N., Pavlova, E.R., Latsis, I.A., Malakhova, M.V., Lavrenova, V.N., Ivchenkov, D.V., Bashkirov, P.V., Kot, E.F., Mineev, K.S., Arseniev, A.S., Klinov, D.V., Lazarev, V.N.

Assembly

Hm-AMP2

Entity

1. Hm-AMP2 (polymer, Thiol state: not present), 13 monomers, 1726.015 Da Detail

EKRWRRLIFN YFX

Formula weight

1726.015 Da

Source organism

Hirudo medicinalis

Exptl. method

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 92.3 %, Completeness: 81.3 %, Completeness (bb): 80.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.3 % (152 of 187)	98.0 % (97 of 99)	57.7 % (41 of 71)	82.4 % (14 of 17)
Backbone	80.6 % (58 of 72)	95.8 % (23 of 24)	69.4 % (25 of 36)	83.3 % (10 of 12)
Sidechain	83.5 % (106 of 127)	98.7 % (74 of 75)	59.6 % (28 of 47)	80.0 % (4 of 5)
Aromatic	47.5 % (19 of 40)	95.0 % (19 of 20)	0.0 % (0 of 19)	0.0 % (0 of 1)
Methyl	100.0 % (8 of 8)	100.0 % (4 of 4)	100.0 % (4 of 4)

1. entity 1

EKRWRRLIFN YFX

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303.0 (±0.1) K, pH 4.8 (±0.1), Details 0.85 mM prok_2590_2 peptide, 85 mM [U-2H] dodecyl phosphocholine, 20 mM [U-2H] sodium acetate, 0.01 % w/w sodium azide, 5 % v/v [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	prok_2590_2 peptide	natural abundance		0.85 mM
2	dodecyl phosphocholine	[U-2H]		85 mM
3	sodium acetate	[U-2H]		20 mM
4	sodium azide	natural abundance		0.01 % w/w
5	D2O	[U-2H]		5 % v/v

Release date

2022-11-21

Citation

Non-toxic antimicrobial peptide Hm-AMP2 from leech metagenome proteins identified by the gradient-boosting approach
Grafskaia, E.N., Pavlova, E.R., Latsis, I.A., Malakhova, M.V., Ivchenkov, D.V., Bashkirov, P.V., Kot, E.F., Mineev, K.S., Arseniev, A.S., Klinov, D.V., Lazarev, V.N.
Materials (2022), 224, 111364, PubMed , DOI 10.1016/j.matdes.2022.111364

Related entities 1. Hm-AMP2, : 1 entities Detail

Experiments performed 5 experiments Detail

1 NOESY

Instrument

Bruker AVANCE II - 700 MHz room temperature triple resonance probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303.0 (±0.1) K, pH 4.8 (±0.1), Details 0.85 mM prok_2590_2 peptide, 85 mM [U-2H] dodecyl phosphocholine, 20 mM [U-2H] sodium acetate, 0.01 % w/w sodium azide, 5 % v/v [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	prok_2590_2 peptide	natural abundance		0.85 mM
2	dodecyl phosphocholine	[U-2H]		85 mM
3	sodium acetate	[U-2H]		20 mM
4	sodium azide	natural abundance		0.01 % w/w
5	D2O	[U-2H]		5 % v/v

2 2D 1H-1H TOCSY

Instrument

Bruker AVANCE II - 700 MHz room temperature triple resonance probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303.0 (±0.1) K, pH 4.8 (±0.1), Details 0.85 mM prok_2590_2 peptide, 85 mM [U-2H] dodecyl phosphocholine, 20 mM [U-2H] sodium acetate, 0.01 % w/w sodium azide, 5 % v/v [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	prok_2590_2 peptide	natural abundance		0.85 mM
2	dodecyl phosphocholine	[U-2H]		85 mM
3	sodium acetate	[U-2H]		20 mM
4	sodium azide	natural abundance		0.01 % w/w
5	D2O	[U-2H]		5 % v/v

3 2D 1H-15N HSQC

Instrument

Bruker AVANCE II - 700 MHz room temperature triple resonance probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303.0 (±0.1) K, pH 4.8 (±0.1), Details 0.85 mM prok_2590_2 peptide, 85 mM [U-2H] dodecyl phosphocholine, 20 mM [U-2H] sodium acetate, 0.01 % w/w sodium azide, 5 % v/v [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	prok_2590_2 peptide	natural abundance		0.85 mM
2	dodecyl phosphocholine	[U-2H]		85 mM
3	sodium acetate	[U-2H]		20 mM
4	sodium azide	natural abundance		0.01 % w/w
5	D2O	[U-2H]		5 % v/v

4 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE II - 700 MHz room temperature triple resonance probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303.0 (±0.1) K, pH 4.8 (±0.1), Details 0.85 mM prok_2590_2 peptide, 85 mM [U-2H] dodecyl phosphocholine, 20 mM [U-2H] sodium acetate, 0.01 % w/w sodium azide, 5 % v/v [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	prok_2590_2 peptide	natural abundance		0.85 mM
2	dodecyl phosphocholine	[U-2H]		85 mM
3	sodium acetate	[U-2H]		20 mM
4	sodium azide	natural abundance		0.01 % w/w
5	D2O	[U-2H]		5 % v/v

5 2D 1H-13C HSQC-TOCSY

Instrument

Bruker AVANCE II - 700 MHz room temperature triple resonance probe

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 303.0 (±0.1) K, pH 4.8 (±0.1), Details 0.85 mM prok_2590_2 peptide, 85 mM [U-2H] dodecyl phosphocholine, 20 mM [U-2H] sodium acetate, 0.01 % w/w sodium azide, 5 % v/v [U-2H] D2O, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	prok_2590_2 peptide	natural abundance		0.85 mM
2	dodecyl phosphocholine	[U-2H]		85 mM
3	sodium acetate	[U-2H]		20 mM
4	sodium azide	natural abundance		0.01 % w/w
5	D2O	[U-2H]		5 % v/v

nullKeywords ANTIMICROBIAL, ANTIMICROBIAL PROTEIN, LEECH, PROTEIN