BMRB: 34717

Structure of the ADP-ribosyltransferase TccC3HVR from Photorhabdus Luminescens

Authors

Lindemann, F., Belyy, A., Friedrich, D., Schmieder, P., Bardiaux, B., Roderer, D., Funk, J., Protze, J., Bieling, P., Oschkinat, H., Raunser, S.

Assembly

TccC3

Entity

1. TccC3 (polymer, Thiol state: not present), 194 monomers, 21695.52 Da Detail

MSTTSTNLQK KSFTLYRADN RSFEEMQSKF PEGFKAWTPL DTKMARQFAS IFIGQKDTSN LPKETVKNIS TWGAKPKLKD LSNYIKYTKD KSTVWVSTAI NTEAGGQSSG APLHKIDMDL YEFAIDGQKL NPLPEGRTKN MVPSLLLDTP QIETSSIIAL NHGPVNDAEI SFLTTIPLKN VKPHKRGTLE VLFQ

Formula weight

21695.52 Da

Source organism

Photorhabdus luminescens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 97.4 %, Completeness: 83.0 %, Completeness (bb): 82.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.0 % (1921 of 2315)	87.5 % (1056 of 1207)	74.4 % (669 of 899)	93.8 % (196 of 209)
Backbone	82.5 % (941 of 1140)	95.6 % (369 of 386)	69.8 % (399 of 572)	95.1 % (173 of 182)
Sidechain	85.3 % (1159 of 1359)	83.7 % (687 of 821)	87.9 % (449 of 511)	85.2 % (23 of 27)
Aromatic	92.9 % (158 of 170)	94.1 % (80 of 85)	91.5 % (75 of 82)	100.0 % (3 of 3)
Methyl	83.7 % (174 of 208)	84.6 % (88 of 104)	82.7 % (86 of 104)

1. entity 1

MSTTSTNLQK KSFTLYRADN RSFEEMQSKF PEGFKAWTPL DTKMARQFAS IFIGQKDTSN LPKETVKNIS TWGAKPKLKD LSNYIKYTKD KSTVWVSTAI NTEAGGQSSG APLHKIDMDL YEFAIDGQKL NPLPEGRTKN MVPSLLLDTP QIETSSIIAL NHGPVNDAEI SFLTTIPLKN VKPHKRGTLE VLFQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 280 K, pH 7.8, Details 500 uM non-exchangeable-D, U-13C, U-15N TcART, 20 mM HEPES, 150 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	TcART	[non-exchangeable-D, U-13C; U-15N]		500 uM
2	HEPES	natural abundance		20 mM
3	sodium chloride	natural abundance		150 mM

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 280 K, pH 7.8, Details 500 uM [U-13C; U-15N] TcART, 20 mM d11-Tris, 150 mM sodium chloride, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	TcART	[U-13C; U-15N]		500 uM
5	sodium chloride	natural abundance		150 mM
6	d11-Tris	natural abundance		20 mM

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 280 K, pH 7.8, Details 500 uM [U-13C; U-15N] TcART, 20 mM HEPES, 150 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
7	TcART	[U-13C; U-15N]		500 uM
8	HEPES	natural abundance		20 mM
9	sodium chloride	natural abundance		150 mM

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Release date

2022-04-18

Citation

Mechanism of threonine ADP-ribosylation of F-actin by a Tc toxin
Belyy, A., Lindemann, F., Roderer, D., Funk, J., Bardiaux, B., Protze, J., Bieling, P., Oschkinat, H., Raunser, S.
Nat. Commun. (2022), 13, 4202-4202, PubMed 35858890 , DOI 10.1038/s41467-022-31836-w , Abstract

Related entities 1. TccC3, : 4 entities Detail

Experiments performed 7 experiments Detail