BMRB: 34732

Solution structure of Pi6, a low affinity blocking kappa-K+-channel peptide from the scorpion Pandinus imperator

Authors

Delepierre, M., Olamendi Portugal, T., Possani, L.D., Guijarro, J.I.

Assembly

Potassium channel toxin kappa-KTx 2.9

Entity

1. Potassium channel toxin kappa-KTx 2.9 (polymer, Thiol state: all disulfide bound), 28 monomers, 3130.510 Da Detail

VDACYEACMH HHMNSDDCIE ACKNPVPP

Formula weight

3130.51 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS4:SG	1:CYS22:SG
2	disulfide	sing	1:CYS8:SG	1:CYS18:SG

Source organism

Pandinus imperator

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.3 %, Completeness (bb): 98.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.3 % (152 of 153)	99.3 % (152 of 153)
Backbone	98.1 % (52 of 53)	98.1 % (52 of 53)
Sidechain	100.0 % (100 of 100)	100.0 % (100 of 100)
Aromatic	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	100.0 % (9 of 9)	100.0 % (9 of 9)

1. entity 1

VDACYEACMH HHMNSDDCIE ACKNPVPP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298.15 K, pH 4, Details 0.19 mM pi6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	pi6	natural abundance		0.19 mM

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Release date

2022-05-10

Citation

Solution structure of Pi6, a low affinity blocking kappa-K+-channel peptide from the scorpion Pandinus imperator
Guijarro, J.I.
Not Published

Related entities 1. Potassium channel toxin kappa-KTx 2.9, : 1 : 1 : 9 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail