BMRBj - BMREntryMaster

Found 1 Document (0.759 seconds)

BMRB: 34746

8AO0

Solution structure of nanoFAST/HBR-DOM2 complex

Authors

Lushpa, V.A., Goncharuk, M.V., Goncharuk, S.A., Baleeva, N.S., Baranov, M.S., Mineev, K.S.

Assembly

Photoactive yellow protein

Entity

1. Photoactive yellow protein, entity 1 (polymer, Thiol state: not present), 112 monomers, 12468.04 Da Detail

MFGAIQLDGD GNILQYNAAX GDITGRDPKQ VIGKNFFKDV APGTDSPEFY GKFKEGVASG NLNTMFEWMI PTSRGPTKVK VHMKKALSGD SYWVFVKRVK LAAALEHHHH HH

2. Photoactive yellow protein, entity O1F (non-polymer), 297.350 Da

Total weight

12765.39 Da

Max. entity weight

12468.04 Da

Source organism

synthetic construct

Exptl. method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 93.1 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.1 % (1214 of 1304)	94.2 % (636 of 675)	91.8 % (468 of 510)	92.4 % (110 of 119)
Backbone	95.3 % (625 of 656)	93.4 % (214 of 229)	96.9 % (311 of 321)	94.3 % (100 of 106)
Sidechain	91.3 % (682 of 747)	94.6 % (422 of 446)	86.8 % (250 of 288)	76.9 % (10 of 13)
Aromatic	69.9 % (102 of 146)	83.6 % (61 of 73)	54.9 % (39 of 71)	100.0 % (2 of 2)
Methyl	100.0 % (104 of 104)	100.0 % (52 of 52)	100.0 % (52 of 52)

1. entity 1

MFGAIQLDGD GNILQYNAAX GDITGRDPKQ VIGKNFFKDV APGTDSPEFY GKFKEGVASG NLNTMFEWMI PTSRGPTKVK VHMKKALSGD SYWVFVKRVK LAAALEHHHH HH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

Release date

2022-11-21

Citation

Spatial Structure of NanoFAST in the Apo State and in Complex with its Fluorogen HBR-DOM2
Lushpa, V.A., Baleeva, N.S., Goncharuk, S.A., Goncharuk, M.V., Arseniev, A.S., Baranov, M.S., Mineev, K.S.
Int. J. Mol. Sci. (2022), 23, 11361-11361, PubMed 36232662 , DOI 10.3390/ijms231911361 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 34747 released on 2022-09-06
Title solution structure of nanoFAST fluorogen-activating protein in the apo state

Related entities 1. Photoactive yellow protein, entity 1, : 1 : 4 : 103 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

2 2D 1H-13C HSQC aliphatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

3 2D 1H-13C HSQC aromatic

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

4 3D HNCO

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

5 3D HNCA

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

6 hbCBcarHar

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

7 3D HNCACB

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

8 3D 1H-15N NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

9 3D 1H-13C NOESY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

10 3D HCCH-TOCSY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

11 3D HCCH-COSY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

12 3D TOCSY

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

13 3D HNCACO

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

14 3D HN(CO)CA

Instrument

Bruker AVANCE III - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 20 mM NaPi, 20 mM sodium chloride, 0.001 % sodium azide, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	NaPi	natural abundance		20 mM
2	sodium chloride	natural abundance		20 mM
3	sodium azide	natural abundance		0.001 %

nullKeywords FAST, FLUORESCENT PROTEIN, binding constatnt, dynamics, fluorogen, fluorogen-activating protein, ligand sepcificity, nanoFAST, spatial structure