BMRB: 34755

NMR structure of the antimicrobial peptide Of-Pis1 in DPC micelles

Authors

Alaimo, N., Bischetti, M., Gallo, M., Cicero, D.O.

Assembly

Piscidin

Entity

1. Piscidin (polymer, Thiol state: not present), 24 monomers, 2578.001 Da Detail

FLGMLLHGVG HAIHGLIHGK QNVE

Formula weight

2578.001 Da

Source organism

Oplegnathus fasciatus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.4 %, Completeness (bb): 78.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	88.4 % (220 of 249)	95.7 % (134 of 140)	78.9 % (86 of 109)
Backbone	78.3 % (94 of 120)	94.3 % (50 of 53)	65.7 % (44 of 67)
Sidechain	98.0 % (145 of 148)	96.6 % (84 of 87)	100.0 % (61 of 61)
Aromatic	100.0 % (26 of 26)	100.0 % (13 of 13)	100.0 % (13 of 13)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. entity 1

FLGMLLHGVG HAIHGLIHGK QNVE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 5 (±0.05), Details 3 mM Of-Pis1, 60 mM [U-2H] DPC, 20 mM sodium phosphate, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Of-Pis1	natural abundance		3 mM
2	DPC	[U-2H]		60 mM
3	sodium phosphate	natural abundance		20 mM

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Release date

2022-10-02

Citation

Structural insights on the selective interaction of the histidine-rich piscidin antimicrobial peptide Of-Pis1 with membranes
Bischetti, M., Alaimo, N., Nardelli, F., Punzi, P., Amiariei, C., Ingenito, R., Musco, G., Gallo, M., Cicero, D.O.
Biochim. Biophys. Acta. Biomembr. (2023), 1865, 184080-184080, PubMed 36328080 , DOI 10.1016/j.bbamem.2022.184080 , Abstract

Related entities 1. Piscidin, : 1 entities Detail

Experiments performed 4 experiments Detail