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Solution structure of the RNA helix formed by the 5'-end of U1 snRNA and an A-1 bulged 5'-splice site in complex with SMN-CY
Authors
Malard, F., Marquevielle, J., Campagne, S.
Assembly
RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3'), RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3')
Entity
1. RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3'), RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3'), entity 1 (polymer, Thiol state: not present), 11 monomers, 3491.046 Da Detail

AUACXXACCU G


2. RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3'), RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3'), entity 2 (polymer, Thiol state: not present), 11 monomers, 3610.134 Da Detail

GGAGUAAGUC U


3. RNA (5'-R(P*AP*UP*AP*CP*(PSU)P*(PSU)P*AP*CP*CP*UP*G)-3'), RNA (5'-R(P*GP*GP*AP*GP*UP*AP*AP*GP*UP*CP*U)-3'), entity 3 (non-polymer), 382.435 Da
Total weight
7483.6147 Da
Max. entity weight
3610.134 Da
Source organism
synthetic construct
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chemcal Shifts
1H: 194, 13C: 147 assignments Detail
IDEntity IDSeq IDComp IDAtom IDAtom typeValVal errAmbig. code
111AH1'H5.8060.0201
211AH2H7.9760.0201
311AH2'H4.6440.0201
411AH3'H4.6760.0201
511AH4'H4.2540.0201
611AH5'H3.8570.0202
711AH5''H3.7920.0202
811AH8H8.1050.0201
911AC1'C90.9420.31
1011AC2C153.1520.31
1111AC2'C76.1180.31
1211AC3'C77.2220.31
1311AC4'C87.0660.31
1411AC5'C63.4350.31
1511AC8C143.1170.31
1612UH1'H5.9320.0201
1712UH2'H4.4820.0201
1812UH3'H4.6190.0201
1912UH4'H4.4770.0201
2012UH5H5.8130.0201
2112UH5'H4.2970.0202
2212UH5''H4.2080.0202
2312UH6H7.8840.0201
2412UC1'C90.4690.31
2512UC2'C75.2980.31
2612UC3'C75.4520.31
2712UC4'C81.7980.31
2812UC5C104.9320.31
2912UC5'C67.2940.31
3012UC6C143.3590.31
3113AH1'H5.7500.0201
3213AH2H7.8290.0201
3313AH2'H4.6710.0201
3413AH3'H4.6110.0201
3513AH4'H4.5290.0201
3613AH5'H4.2130.0202
3713AH5''H4.4490.0202
3813AH8H8.2720.0201
3913AC1'C92.4640.31
4013AC2C153.5290.31
4113AC2'C75.2400.31
4213AC3'C73.4310.31
4313AC4'C82.1100.31
4413AC5'C66.0210.31
4513AC8C140.5640.31
4614CH1'H5.4640.0201
4714CH2'H4.6490.0201
4814CH3'H4.4760.0201
4914CH4'H4.4250.0201
5014CH5H5.2660.0201
5114CH5'H4.4020.0202
5214CH5''H4.0900.0202
5314CH6H7.5200.0201
5414CC1'C93.5720.31
5514CC2'C74.8640.31
5614CC3'C72.3120.31
5714CC4'C82.9720.31
5814CC5C97.1250.31
5914CC5'C65.3820.31
6014CC6C141.0880.31
6115PSUH1'H4.8100.0201
6215PSUH2'H4.5760.0201
6315PSUH3'H4.4870.0201
6415PSUH4'H4.2290.0201
6515PSUH5'H4.4250.0202
6615PSUH5''H4.0250.0202
6715PSUH6H7.4230.0201
6815PSUC1'C82.8420.31
6915PSUC2'C75.1490.31
7015PSUC3'C74.5700.31
7115PSUC4'C79.5170.31
7215PSUC5'C65.4640.31
7315PSUC6C140.7760.31
7415PSUHN3H10.5040.0201
7516PSUH1'H4.7450.0201
7616PSUH2'H4.5940.0201
7716PSUH3'H4.4810.0201
7816PSUH4'H4.2720.0201
7916PSUH5'H4.4220.0202
8016PSUH5''H4.0540.0202
8116PSUH6H7.4920.0201
8216PSUC1'C82.6540.31
8316PSUC2'C75.2590.31
8416PSUC3'C75.0970.31
8516PSUC4'C79.5170.31
8616PSUC5'C66.2140.31
8716PSUC6C140.8190.31
8816PSUHN3H10.7840.0201
8917AH1'H5.9180.0201
9017AH2H7.0350.0201
9117AH2'H4.6490.0201
9217AH3'H4.6660.0201
9317AH4'H4.3980.0201
9417AH5'H4.3900.0202
9517AH5''H4.1170.0202
9617AH8H8.0870.0201
9717AC1'C92.5310.31
9817AC2C152.9640.31
9917AC2'C72.1300.31
10017AC3'C72.1660.31
10117AC4'C83.0800.31
10217AC5'C66.4390.31
10317AC8C139.5660.31
10418CH1'H5.2820.0201
10518CH2'H4.4580.0201
10618CH3'H4.2340.0201
10718CH4'H4.3270.0201
10818CH5H5.1200.0201
10918CH5'H4.0090.0202
11018CH5''H4.5040.0202
11118CH6H7.3210.0201
11218CC1'C93.5590.31
11318CC2'C75.3100.31
11418CC3'C71.6550.31
11518CC4'C81.5700.31
11618CC5C96.8370.31
11718CC5'C64.3420.31
11818CC6C140.3160.31
11919CH1'H5.6500.0201
12019CH2'H4.4160.0201
12119CH3'H4.2600.0201
12219CH4'H4.3230.0201
12319CH5H5.5670.0201
12419CH5'H4.4410.0202
12519CH5''H4.0340.0202
12619CH6H7.6880.0201
12719CC1'C93.1270.31
12819CC2'C73.8580.31
12919CC3'C71.5510.31
13019CC4'C85.6190.31
13119CC5C98.3090.31
13219CC5'C64.4450.31
13319CC6C141.3040.31
134110UH1'H5.5350.0201
135110UH2'H4.4520.0201
136110UH3'H4.2880.0201
137110UH4'H4.0250.0201
138110UH5H5.5650.0201
139110UH5'H4.1890.0202
140110UH5''H4.0380.0202
141110UH6H7.6920.0201
142110UC1'C91.8500.31
143110UC2'C75.3060.31
144110UC3'C72.2200.31
145110UC4'C85.1670.31
146110UC5C104.5920.31
147110UC5'C64.3360.31
148110UC6C141.8490.31
149111GH1'H5.6770.0201
150111GH2'H4.4400.0201
151111GH3'H4.3320.0201
152111GH4'H4.2120.0201
153111GH5'H4.1880.0202
154111GH5''H4.0530.0202
155111GH8H7.8360.0201
156111GC1'C90.0190.31
157111GC2'C76.6240.31
158111GC3'C72.0550.31
159111GC4'C85.4810.31
160111GC5'C66.7990.31
161111GC8C139.3700.31
16221GH1'H5.6780.0201
16321GH2'H4.6880.0201
16421GH3'H4.6640.0201
16521GH4'H4.4160.0201
16621GH5'H3.8040.0202
16721GH5''H3.7500.0202
16821GH8H7.8080.0201
16921GC1'C91.1510.31
17021GC2'C75.1560.31
17121GC3'C76.9720.31
17221GC4'C87.0070.31
17321GC5'C63.5340.31
17421GC8C140.6560.31
17522GH1'H5.8800.0201
17622GH2'H4.8740.0201
17722GH3'H4.8060.0201
17822GH4'H4.5470.0201
17922GH5'H4.2920.0202
18022GH5''H4.1900.0202
18122GH8H7.9120.0201
18222GC1'C90.0530.31
18322GC2'C76.1960.31
18422GC3'C75.6470.31
18522GC4'C84.6450.31
18622GC5'C67.4570.31
18722GC8C138.8170.31
18823AH1'H5.9860.0201
18923AH2H8.1440.0201
19023AH2'H4.8150.0201
19123AH3'H4.6850.0201
19223AH4'H4.6150.0201
19323AH5'H4.4320.0202
19423AH5''H4.2910.0202
19523AH8H8.2790.0201
19623AC1'C91.8460.31
19723AC2C153.1270.31
19823AC2'C75.6030.31
19923AC3'C77.1110.31
20023AC4'C83.6710.31
20123AC5'C67.4780.31
20223AC8C142.7980.31
20324GH1'H5.5300.0201
20424GH2'H4.7230.0201
20524GH3'H4.5170.0201
20624GH4'H4.6170.0201
20724GH5'H4.4290.0202
20824GH5''H4.1460.0202
20924GH8H7.3830.0201
21024GC1'C92.9240.31
21124GC2'C74.8440.31
21224GC3'C72.7850.31
21324GC4'C84.7170.31
21424GC5'C65.6990.31
21524GC8C136.6590.31
21625UH1'H5.5220.0201
21725UH2'H4.5880.0201
21825UH3H13.6480.0201
21925UH3'H4.5570.0201
22025UH4'H4.3250.0201
22125UH5H5.1140.0201
22225UH5'H4.5630.0202
22325UH5''H4.1400.0202
22425UH6H7.7550.0201
22525UC1'C93.4610.31
22625UC2'C73.2430.31
22725UC3'C74.7260.31
22825UC4'C83.5020.31
22925UC5C102.6490.31
23025UC5'C64.2380.31
23125UC6C141.3940.31
23226AH1'H5.9400.0201
23326AH2H6.4330.0201
23426AH2'H4.7290.0201
23526AH3'H4.5660.0201
23626AH4'H4.4490.0201
23726AH5'H4.1720.0202
23826AH5''H4.3500.0202
23926AH8H8.0730.0201
24026AC1'C92.2170.31
24126AC2C151.9090.31
24226AC2'C73.0290.31
24326AC3'C74.5490.31
24426AC4'C81.8250.31
24526AC5'C66.8020.31
24626AC8C139.5320.31
24727AH1'H5.7810.0201
24827AH2H7.3240.0201
24927AH2'H4.6070.0201
25027AH3'H4.4920.0201
25127AH4'H4.5420.0201
25227AH5'H4.1280.0202
25327AH5''H4.2980.0202
25427AH8H7.6440.0201
25527AC1'C92.4810.31
25627AC2C152.7920.31
25727AC2'C76.2510.31
25827AC3'C72.3800.31
25927AC4'C82.2470.31
26027AC5'C67.2500.31
26127AC8C138.9640.31
26228GH1H12.5430.0201
26328GH1'H5.5370.0201
26428GH2'H4.5590.0201
26528GH3'H4.5670.0201
26628GH4'H4.4090.0201
26728GH5'H4.3540.0202
26828GH5''H4.0010.0202
26928GH8H7.0520.0201
27028GC1'C92.7640.31
27128GC2'C75.4510.31
27228GC3'C71.9280.31
27328GC4'C81.9280.31
27428GC5'C65.5420.31
27528GC8C135.6700.31
27629UH1'H5.6940.0201
27729UH2'H4.4620.0201
27829UH3'H4.3960.0201
27929UH4'H4.5130.0201
28029UH5H5.0160.0201
28129UH5'H4.3520.0202
28229UH5''H4.0460.0202
28329UH6H7.5530.0201
28429UC1'C92.6320.31
28529UC2'C74.2240.31
28629UC3'C74.7510.31
28729UC4'C82.5510.31
28829UC5C102.8200.31
28929UC5'C65.6800.31
29029UC6C141.4290.31
291210CH1'H5.7100.0201
292210CH2'H4.4180.0201
293210CH3'H4.2200.0201
294210CH4'H4.3230.0201
295210CH5H5.8140.0201
296210CH5'H4.0920.0202
297210CH5''H4.0760.0202
298210CH6H7.7720.0201
299210CC1'C92.7010.31
300210CC2'C75.6190.31
301210CC3'C75.5010.31
302210CC4'C82.4320.31
303210CC5C98.0540.31
304210CC5'C66.7900.31
305210CC6C142.7270.31
306211UH1'H5.7450.0201
307211UH2'H4.4290.0201
308211UH3'H4.2080.0201
309211UH4'H4.1410.0201
310211UH5H5.6670.0201
311211UH5'H4.1100.0201
312211UH5''H4.1100.0201
313211UH6H7.7450.0201
314211UC1'C91.0090.31
315211UC2'C75.3920.31
316211UC3'C71.3690.31
317211UC4'C85.0200.31
318211UC5C104.5640.31
319211UC5'C66.3860.31
320211UC6C143.2980.31
32131ULRH28AH2.2470.0201
32231ULRH28BH2.2470.0201
32331ULRH28CH2.2470.0201
32431ULRH25H7.3920.0201
32531ULRH21H8.5810.0201
32631ULRH24H8.2270.0201
32731ULRH13H7.2860.0201
32831ULRH12H6.3510.0201
32931ULRH3AH2.9880.0202
33031ULRH3BH2.9880.0202
33131ULRH4AH3.1590.0202
33231ULRH4BH3.1060.0202
33331ULRH1AH3.6810.0202
33431ULRH1BH3.6810.0202
33531ULRH6H2.7780.0202
33631ULRH14AH1.6540.0202
33731ULRH14BH1.6540.0201
33831ULRH15AH0.9740.0201
33931ULRH15BH0.9740.0201
34031ULRH15CH0.9740.0201
34131ULRH10H6.2480.0201

Release date
2023-03-01
Citation
The diversity of splicing modifiers acting on A-1 bulged 5'-splice sites reveals rules to guide rational design
Malard, F., Marquevielle, J., Campagne, S.
Experiments performed 4 experiments Detail
Chemical shift validation 5 contents Detail
Keywords SPLICING, Splicing correction, U1 snRNP, bulged 5'-splice site, molecular glue