BMRB: 34806

Solution NMR structure of Notch1 L1740-1743 TMD

Authors

Guschtschin-Schmidt, N., Muhle-Goll, C.

Assembly

Notch 1 extracellular truncation

Entity

1. Notch 1 extracellular truncation (polymer), 30 monomers, 3526.515 Da Detail

KKKLHFMYVL LLLFVLLFFV GCGVLLSKKK

Formula weight

3526.515 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 219, ¹³C: 112, ¹⁵N: 29 assignments Detail

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ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	1	LYS	H	H	8.197
2	1	1	LYS	HA	H	4.148
3	1	1	LYS	HB2	H	1.840
4	1	1	LYS	HG2	H	1.571
5	1	1	LYS	HG3	H	1.477
6	1	1	LYS	HD2	H	1.750
7	1	1	LYS	HE2	H	3.001
8	1	1	LYS	CA	C	58.571
9	1	1	LYS	CB	C	32.087
10	1	1	LYS	CG	C	24.432
11	1	1	LYS	CD	C	28.582
12	1	1	LYS	CE	C	41.555
13	1	1	LYS	N	N	118.546
14	1	2	LYS	H	H	8.531
15	1	2	LYS	HA	H	4.147
16	1	2	LYS	HB2	H	1.859
17	1	2	LYS	HG2	H	1.467
18	1	2	LYS	HG3	H	1.577
19	1	2	LYS	HD2	H	1.734
20	1	2	LYS	HE2	H	2.997
21	1	2	LYS	CA	C	58.571
22	1	2	LYS	CB	C	32.199
23	1	2	LYS	CG	C	24.432
24	1	2	LYS	CD	C	28.582
25	1	2	LYS	CE	C	41.555
26	1	2	LYS	N	N	120.306
27	1	3	LYS	H	H	7.897
28	1	3	LYS	HA	H	4.205
29	1	3	LYS	HB2	H	1.856
30	1	3	LYS	HB3	H	1.914
31	1	3	LYS	HG2	H	1.573
32	1	3	LYS	HD2	H	1.749
33	1	3	LYS	HE2	H	3.001
34	1	3	LYS	CA	C	58.333
35	1	3	LYS	CB	C	32.218
36	1	3	LYS	CG	C	24.448
37	1	3	LYS	CD	C	28.582
38	1	3	LYS	CE	C	41.555
39	1	3	LYS	N	N	120.583
40	1	4	LEU	H	H	7.747
41	1	4	LEU	HA	H	4.234
42	1	4	LEU	HB2	H	1.709
43	1	4	LEU	HB3	H	1.765
44	1	4	LEU	HG	H	1.651
45	1	4	LEU	HD11	H	0.961
46	1	4	LEU	HD12	H	0.961
47	1	4	LEU	HD13	H	0.961
48	1	4	LEU	HD21	H	0.923
49	1	4	LEU	HD22	H	0.923
50	1	4	LEU	HD23	H	0.923
51	1	4	LEU	CA	C	56.784
52	1	4	LEU	CB	C	41.173
53	1	4	LEU	CG	C	26.123
54	1	4	LEU	CD1	C	23.235
55	1	4	LEU	N	N	118.592
56	1	5	HIS	H	H	7.984
57	1	5	HIS	HA	H	4.382
58	1	5	HIS	HB2	H	3.219
59	1	5	HIS	CA	C	58.703
60	1	5	HIS	CB	C	28.703
61	1	5	HIS	N	N	119.388
62	1	6	PHE	H	H	7.888
63	1	6	PHE	HA	H	4.333
64	1	6	PHE	HB2	H	3.236
65	1	6	PHE	HD1	H	7.251
66	1	6	PHE	HD2	H	7.251
67	1	6	PHE	HE1	H	7.318
68	1	6	PHE	HE2	H	7.318
69	1	6	PHE	CA	C	60.103
70	1	6	PHE	CB	C	38.233
71	1	6	PHE	CD2	C	128.889
72	1	6	PHE	CE2	C	130.350
73	1	7	MET	H	H	8.121
74	1	7	MET	HA	H	4.121
75	1	7	MET	HB2	H	2.124
76	1	7	MET	HB3	H	2.206
77	1	7	MET	HG2	H	2.572
78	1	7	MET	HG3	H	2.681
79	1	7	MET	CA	C	57.802
80	1	7	MET	CB	C	31.373
81	1	7	MET	N	N	117.091
82	1	8	TYR	H	H	7.915
83	1	8	TYR	HA	H	4.279
84	1	8	TYR	HB2	H	3.165
85	1	8	TYR	HD1	H	7.090
86	1	8	TYR	HD2	H	7.090
87	1	8	TYR	HE1	H	6.772
88	1	8	TYR	HE2	H	6.772
89	1	8	TYR	CA	C	60.562
90	1	8	TYR	CB	C	37.349
91	1	8	TYR	CD1	C	131.864
92	1	8	TYR	CE1	C	117.136
93	1	8	TYR	N	N	118.651
94	1	9	VAL	H	H	7.763
95	1	9	VAL	HA	H	3.561
96	1	9	VAL	HB	H	2.158
97	1	9	VAL	HG11	H	0.994
98	1	9	VAL	HG12	H	0.994
99	1	9	VAL	HG13	H	0.994
100	1	9	VAL	HG21	H	0.939
101	1	9	VAL	HG22	H	0.939
102	1	9	VAL	HG23	H	0.939
103	1	9	VAL	CA	C	66.030
104	1	9	VAL	CB	C	30.823
105	1	9	VAL	CG1	C	21.366
106	1	9	VAL	CG2	C	19.826
107	1	9	VAL	N	N	118.592
108	1	10	LEU	H	H	7.787
109	1	10	LEU	HA	H	3.985
110	1	10	LEU	HB2	H	1.470
111	1	10	LEU	HB3	H	1.746
112	1	10	LEU	HG	H	1.565
113	1	10	LEU	HD11	H	0.816
114	1	10	LEU	HD12	H	0.816
115	1	10	LEU	HD13	H	0.816
116	1	10	LEU	CA	C	57.418
117	1	10	LEU	CB	C	40.907
118	1	10	LEU	CG	C	25.929
119	1	10	LEU	CD1	C	23.687
120	1	10	LEU	N	N	118.768
121	1	11	LEU	H	H	7.828
122	1	11	LEU	HA	H	4.153
123	1	11	LEU	HB2	H	1.802
124	1	11	LEU	HB3	H	1.754
125	1	11	LEU	HD11	H	0.919
126	1	11	LEU	HD12	H	0.919
127	1	11	LEU	HD13	H	0.919
128	1	11	LEU	HD21	H	0.888
129	1	11	LEU	HD22	H	0.888
130	1	11	LEU	HD23	H	0.888
131	1	11	LEU	CA	C	58.015
132	1	11	LEU	CB	C	40.927
133	1	11	LEU	CD1	C	22.914
134	1	11	LEU	CD2	C	22.867
135	1	11	LEU	N	N	119.302
136	1	12	LEU	H	H	7.918
137	1	12	LEU	HA	H	4.048
138	1	12	LEU	HB2	H	1.725
139	1	12	LEU	HD11	H	0.864
140	1	12	LEU	HD12	H	0.864
141	1	12	LEU	HD13	H	0.864
142	1	12	LEU	CA	C	57.535
143	1	12	LEU	CB	C	40.909
144	1	12	LEU	CD1	C	22.579
145	1	12	LEU	N	N	119.550
146	1	13	LEU	H	H	8.252
147	1	13	LEU	HA	H	4.123
148	1	13	LEU	HB2	H	1.688
149	1	13	LEU	HB3	H	1.826
150	1	13	LEU	HD11	H	0.912
151	1	13	LEU	HD12	H	0.912
152	1	13	LEU	HD13	H	0.912
153	1	13	LEU	HD21	H	0.866
154	1	13	LEU	HD22	H	0.866
155	1	13	LEU	HD23	H	0.866
156	1	13	LEU	CA	C	57.544
157	1	13	LEU	CB	C	40.994
158	1	13	LEU	CD1	C	23.189
159	1	13	LEU	CD2	C	22.989
160	1	13	LEU	N	N	118.753
161	1	14	PHE	H	H	8.205
162	1	14	PHE	HA	H	4.293
163	1	14	PHE	HB2	H	3.387
164	1	14	PHE	HB3	H	3.353
165	1	14	PHE	HD1	H	7.224
166	1	14	PHE	HD2	H	7.224
167	1	14	PHE	HE1	H	7.189
168	1	14	PHE	HE2	H	7.189
169	1	14	PHE	CA	C	60.803
170	1	14	PHE	CB	C	37.958
171	1	14	PHE	CD1	C	130.708
172	1	14	PHE	CE1	C	130.092
173	1	14	PHE	N	N	118.546
174	1	15	VAL	H	H	8.385
175	1	15	VAL	HA	H	3.694
176	1	15	VAL	HB	H	2.382
177	1	15	VAL	HG11	H	1.058
178	1	15	VAL	HG12	H	1.058
179	1	15	VAL	HG13	H	1.058
180	1	15	VAL	HG21	H	1.185
181	1	15	VAL	HG22	H	1.185
182	1	15	VAL	HG23	H	1.185
183	1	15	VAL	CA	C	66.394
184	1	15	VAL	CB	C	31.195
185	1	15	VAL	CG1	C	21.437
186	1	15	VAL	CG2	C	20.331
187	1	15	VAL	N	N	120.182
188	1	16	LEU	H	H	8.484
189	1	16	LEU	HA	H	4.240
190	1	16	LEU	HB2	H	1.933
191	1	16	LEU	HB3	H	1.831
192	1	16	LEU	HD11	H	0.925
193	1	16	LEU	HD12	H	0.925
194	1	16	LEU	HD13	H	0.925
195	1	16	LEU	CA	C	58.147
196	1	16	LEU	CB	C	41.177
197	1	16	LEU	CD1	C	22.406
198	1	16	LEU	N	N	120.437
199	1	17	LEU	H	H	8.646
200	1	17	LEU	HA	H	4.072
201	1	17	LEU	HB2	H	1.401
202	1	17	LEU	HB3	H	1.919
203	1	17	LEU	HD11	H	0.879
204	1	17	LEU	HD12	H	0.879
205	1	17	LEU	HD13	H	0.879
206	1	17	LEU	HD21	H	0.850
207	1	17	LEU	HD22	H	0.850
208	1	17	LEU	HD23	H	0.850
209	1	17	LEU	CA	C	57.629
210	1	17	LEU	CB	C	40.886
211	1	17	LEU	CD1	C	23.675
212	1	17	LEU	CD2	C	21.283
213	1	17	LEU	N	N	117.445
214	1	18	PHE	H	H	8.290
215	1	18	PHE	HA	H	4.220
216	1	18	PHE	HB2	H	3.163
217	1	18	PHE	HB3	H	2.930
218	1	18	PHE	HD1	H	6.826
219	1	18	PHE	HD2	H	6.826
220	1	18	PHE	HE1	H	7.106
221	1	18	PHE	HE2	H	7.106
222	1	18	PHE	CA	C	60.492
223	1	18	PHE	CB	C	38.080
224	1	18	PHE	CD2	C	130.988
225	1	18	PHE	CE1	C	130.208
226	1	18	PHE	N	N	121.131
227	1	19	PHE	H	H	8.447
228	1	19	PHE	HA	H	4.301
229	1	19	PHE	HB2	H	3.311
230	1	19	PHE	HD1	H	7.405
231	1	19	PHE	HD2	H	7.405
232	1	19	PHE	HE1	H	7.360
233	1	19	PHE	HE2	H	7.360
234	1	19	PHE	CA	C	61.213
235	1	19	PHE	CB	C	38.140
236	1	19	PHE	CD1	C	130.764
237	1	19	PHE	CE2	C	130.435
238	1	19	PHE	N	N	119.960
239	1	20	VAL	H	H	8.922
240	1	20	VAL	HA	H	3.735
241	1	20	VAL	HB	H	2.284
242	1	20	VAL	HG11	H	0.952
243	1	20	VAL	HG12	H	0.952
244	1	20	VAL	HG13	H	0.952
245	1	20	VAL	HG21	H	1.142
246	1	20	VAL	HG22	H	1.142
247	1	20	VAL	HG23	H	1.142
248	1	20	VAL	CA	C	65.960
249	1	20	VAL	CB	C	31.136
250	1	20	VAL	CG1	C	19.732
251	1	20	VAL	CG2	C	21.355
252	1	20	VAL	N	N	121.409
253	1	21	GLY	H	H	8.219
254	1	21	GLY	HA2	H	3.923
255	1	21	GLY	HA3	H	3.750
256	1	21	GLY	CA	C	46.342
257	1	21	GLY	N	N	107.043
258	1	22	CYS	H	H	7.955
259	1	22	CYS	HA	H	4.074
260	1	22	CYS	HB2	H	2.646
261	1	22	CYS	HB3	H	2.950
262	1	22	CYS	CA	C	62.503
263	1	22	CYS	CB	C	25.719
264	1	22	CYS	N	N	118.106
265	1	23	GLY	H	H	7.964
266	1	23	GLY	HA2	H	3.782
267	1	23	GLY	HA3	H	3.870
268	1	23	GLY	CA	C	46.344
269	1	23	GLY	N	N	106.949
270	1	24	VAL	H	H	8.138
271	1	24	VAL	HA	H	3.652
272	1	24	VAL	HB	H	2.244
273	1	24	VAL	HG11	H	1.051
274	1	24	VAL	HG12	H	1.051
275	1	24	VAL	HG13	H	1.051
276	1	24	VAL	HG21	H	0.923
277	1	24	VAL	HG22	H	0.923
278	1	24	VAL	HG23	H	0.923
279	1	24	VAL	CA	C	65.907
280	1	24	VAL	CB	C	30.977
281	1	24	VAL	CG1	C	20.865
282	1	24	VAL	CG2	C	19.798
283	1	24	VAL	N	N	122.810
284	1	25	LEU	H	H	7.929
285	1	25	LEU	HA	H	4.068
286	1	25	LEU	HB2	H	1.650
287	1	25	LEU	HB3	H	1.847
288	1	25	LEU	HG	H	1.721
289	1	25	LEU	HD11	H	0.918
290	1	25	LEU	HD12	H	0.918
291	1	25	LEU	HD13	H	0.918
292	1	25	LEU	HD21	H	0.872
293	1	25	LEU	HD22	H	0.872
294	1	25	LEU	HD23	H	0.872
295	1	25	LEU	CA	C	57.415
296	1	25	LEU	CB	C	41.146
297	1	25	LEU	CG	C	26.324
298	1	25	LEU	CD1	C	22.914
299	1	25	LEU	CD2	C	22.234
300	1	25	LEU	N	N	119.550
301	1	26	LEU	H	H	8.340
302	1	26	LEU	HA	H	4.111
303	1	26	LEU	HB2	H	1.824
304	1	26	LEU	HB3	H	1.861
305	1	26	LEU	HG	H	1.598
306	1	26	LEU	HD11	H	0.890
307	1	26	LEU	HD12	H	0.890
308	1	26	LEU	HD13	H	0.890
309	1	26	LEU	HD21	H	0.898
310	1	26	LEU	HD22	H	0.898
311	1	26	LEU	HD23	H	0.898
312	1	26	LEU	CA	C	56.961
313	1	26	LEU	CB	C	41.083
314	1	26	LEU	CG	C	26.315
315	1	26	LEU	CD1	C	21.843
316	1	26	LEU	CD2	C	23.384
317	1	26	LEU	N	N	118.109
318	1	27	SER	H	H	7.945
319	1	27	SER	HA	H	4.225
320	1	27	SER	HB2	H	4.031
321	1	27	SER	CA	C	60.492
322	1	27	SER	CB	C	62.557
323	1	27	SER	N	N	113.658
324	1	28	LYS	H	H	7.857
325	1	28	LYS	HA	H	4.267
326	1	28	LYS	HB2	H	1.975
327	1	28	LYS	HG2	H	1.582
328	1	28	LYS	HD2	H	1.696
329	1	28	LYS	HE2	H	2.950
330	1	28	LYS	CA	C	56.577
331	1	28	LYS	CB	C	31.497
332	1	28	LYS	CG	C	24.448
333	1	28	LYS	CD	C	28.111
334	1	28	LYS	CE	C	41.621
335	1	28	LYS	N	N	121.340
336	1	29	LYS	H	H	7.994
337	1	29	LYS	HA	H	4.249
338	1	29	LYS	HB2	H	1.934
339	1	29	LYS	HG2	H	1.555
340	1	29	LYS	HD2	H	1.708
341	1	29	LYS	HE2	H	2.996
342	1	29	LYS	CA	C	56.485
343	1	29	LYS	CB	C	31.735
344	1	29	LYS	CG	C	23.931
345	1	29	LYS	CD	C	28.111
346	1	29	LYS	CE	C	41.372
347	1	29	LYS	N	N	119.388
348	1	30	LYS	H	H	7.920
349	1	30	LYS	HA	H	4.295
350	1	30	LYS	HB2	H	1.871
351	1	30	LYS	HB3	H	1.926
352	1	30	LYS	HG2	H	1.532
353	1	30	LYS	HD2	H	1.710
354	1	30	LYS	HE2	H	2.996
355	1	30	LYS	CA	C	55.582
356	1	30	LYS	CB	C	31.692
357	1	30	LYS	CG	C	23.902
358	1	30	LYS	CD	C	28.111
359	1	30	LYS	CE	C	41.372
360	1	30	LYS	N	N	120.583

Release date

2023-05-10

Citation

Permissive Conformations of a Transmembrane Helix Allow Intramembrane Proteolysis by γ-Secretase
Ortner, M., Guschtschin-Schmidt, N., Stelzer, W., Muhle-Goll, C., Langosch, D.
J. Mol. Biol. (2023), 435, 168218-168218, PubMed 37536392 , DOI 10.1016/j.jmb.2023.168218 , Abstract

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