BMRBj - BMREntryMaster

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val
1	1	1	LYS	H	H	8.118
2	1	1	LYS	HA	H	4.146
3	1	1	LYS	HB2	H	1.809
4	1	1	LYS	HB3	H	1.849
5	1	1	LYS	HG2	H	1.480
6	1	1	LYS	HG3	H	1.547
7	1	1	LYS	HD2	H	1.732
8	1	1	LYS	HE2	H	2.999
9	1	1	LYS	CA	C	57.853
10	1	1	LYS	CB	C	32.110
11	1	1	LYS	CG	C	24.423
12	1	1	LYS	CD	C	28.576
13	1	1	LYS	CE	C	41.563
14	1	1	LYS	N	N	125.959
15	1	2	LYS	H	H	8.488
16	1	2	LYS	HA	H	4.142
17	1	2	LYS	HB2	H	1.852
18	1	2	LYS	HG2	H	1.444
19	1	2	LYS	HG3	H	1.563
20	1	2	LYS	HD2	H	1.723
21	1	2	LYS	HE2	H	2.999
22	1	2	LYS	CA	C	58.259
23	1	2	LYS	CB	C	31.760
24	1	2	LYS	CG	C	24.432
25	1	2	LYS	CD	C	28.563
26	1	2	LYS	CE	C	41.563
27	1	2	LYS	N	N	119.374
28	1	3	LYS	H	H	7.886
29	1	3	LYS	HA	H	4.192
30	1	3	LYS	HB2	H	1.898
31	1	3	LYS	HG2	H	1.557
32	1	3	LYS	HG3	H	1.486
33	1	3	LYS	HD2	H	1.738
34	1	3	LYS	HE2	H	2.999
35	1	3	LYS	CA	C	58.096
36	1	3	LYS	CB	C	31.802
37	1	3	LYS	CG	C	23.940
38	1	3	LYS	CD	C	28.576
39	1	3	LYS	CE	C	41.563
40	1	3	LYS	N	N	118.824
41	1	4	LEU	H	H	7.725
42	1	4	LEU	HA	H	4.228
43	1	4	LEU	HB2	H	1.748
44	1	4	LEU	HB3	H	1.699
45	1	4	LEU	HG	H	1.617
46	1	4	LEU	HD11	H	0.916
47	1	4	LEU	HD12	H	0.916
48	1	4	LEU	HD13	H	0.916
49	1	4	LEU	HD21	H	0.958
50	1	4	LEU	HD22	H	0.958
51	1	4	LEU	HD23	H	0.958
52	1	4	LEU	CA	C	56.581
53	1	4	LEU	CB	C	41.192
54	1	4	LEU	CG	C	26.412
55	1	4	LEU	CD1	C	22.322
56	1	4	LEU	CD2	C	23.414
57	1	4	LEU	N	N	116.864
58	1	5	HIS	H	H	7.986
59	1	5	HIS	HA	H	4.331
60	1	5	HIS	HB2	H	3.153
61	1	5	HIS	HD1	H	6.719
62	1	5	HIS	CA	C	58.694
63	1	5	HIS	CB	C	29.230
64	1	5	HIS	N	N	117.428
65	1	6	PHE	H	H	7.864
66	1	6	PHE	HA	H	4.315
67	1	6	PHE	HB2	H	3.206
68	1	6	PHE	HD1	H	7.225
69	1	6	PHE	HD2	H	7.225
70	1	6	PHE	CA	C	59.806
71	1	6	PHE	CB	C	38.197
72	1	6	PHE	CD1	C	129.053
73	1	6	PHE	N	N	117.823
74	1	7	MET	H	H	8.176
75	1	7	MET	HA	H	4.147
76	1	7	MET	HB2	H	2.025
77	1	7	MET	HB3	H	2.154
78	1	7	MET	HG2	H	2.500
79	1	7	MET	HG3	H	2.592
80	1	7	MET	CA	C	57.232
81	1	7	MET	CB	C	31.718
82	1	7	MET	CG	C	31.373
83	1	7	MET	N	N	118.334
84	1	8	TYR	H	H	8.102
85	1	8	TYR	HA	H	4.371
86	1	8	TYR	HB2	H	3.079
87	1	8	TYR	HB3	H	3.127
88	1	8	TYR	HD1	H	7.080
89	1	8	TYR	HD2	H	7.080
90	1	8	TYR	HE1	H	6.778
91	1	8	TYR	HE2	H	6.778
92	1	8	TYR	CA	C	59.614
93	1	8	TYR	CB	C	37.953
94	1	8	TYR	CD2	C	132.050
95	1	8	TYR	N	N	119.147
96	1	9	VAL	H	H	8.013
97	1	9	VAL	HA	H	3.912
98	1	9	VAL	HB	H	2.079
99	1	9	VAL	HG11	H	0.916
100	1	9	VAL	HG12	H	0.916
101	1	9	VAL	HG13	H	0.916
102	1	9	VAL	CA	C	63.577
103	1	9	VAL	CB	C	31.442
104	1	9	VAL	CG1	C	19.864
105	1	9	VAL	N	N	117.536
106	1	10	GLY	H	H	7.977
107	1	10	GLY	HA2	H	3.795
108	1	10	GLY	HA3	H	3.928
109	1	10	GLY	CA	C	45.012
110	1	10	GLY	N	N	107.799
111	1	11	GLY	H	H	7.864
112	1	11	GLY	HA2	H	3.932
113	1	11	GLY	CA	C	45.140
114	1	11	GLY	N	N	107.532
115	1	12	GLY	H	H	8.095
116	1	12	GLY	HA2	H	3.855
117	1	12	GLY	CA	C	45.690
118	1	12	GLY	N	N	107.330
119	1	13	GLY	H	H	8.152
120	1	13	GLY	HA2	H	3.810
121	1	13	GLY	HA3	H	3.773
122	1	13	GLY	CA	C	45.824
123	1	13	GLY	N	N	107.251
124	1	14	PHE	H	H	7.650
125	1	14	PHE	HA	H	4.385
126	1	14	PHE	HB2	H	3.168
127	1	14	PHE	HD1	H	7.245
128	1	14	PHE	HD2	H	7.245
129	1	14	PHE	HE1	H	7.273
130	1	14	PHE	HE2	H	7.273
131	1	14	PHE	CA	C	59.865
132	1	14	PHE	CB	C	38.099
133	1	14	PHE	CD1	C	130.790
134	1	14	PHE	N	N	118.740
135	1	15	VAL	H	H	7.493
136	1	15	VAL	HA	H	3.710
137	1	15	VAL	HB	H	2.186
138	1	15	VAL	HG11	H	1.033
139	1	15	VAL	HG12	H	1.033
140	1	15	VAL	HG13	H	1.033
141	1	15	VAL	CA	C	65.431
142	1	15	VAL	CB	C	31.139
143	1	15	VAL	CG1	C	20.830
144	1	15	VAL	N	N	118.736
145	1	16	LEU	H	H	7.517
146	1	16	LEU	HA	H	4.254
147	1	16	LEU	HB2	H	1.769
148	1	16	LEU	HD11	H	0.933
149	1	16	LEU	HD12	H	0.933
150	1	16	LEU	HD13	H	0.933
151	1	16	LEU	HD21	H	0.990
152	1	16	LEU	HD22	H	0.990
153	1	16	LEU	HD23	H	0.990
154	1	16	LEU	CA	C	57.682
155	1	16	LEU	CB	C	41.161
156	1	16	LEU	CD1	C	22.898
157	1	16	LEU	CD2	C	22.888
158	1	16	LEU	N	N	119.136
159	1	17	LEU	H	H	7.771
160	1	17	LEU	HA	H	4.060
161	1	17	LEU	HB2	H	1.837
162	1	17	LEU	HB3	H	1.594
163	1	17	LEU	HG	H	1.781
164	1	17	LEU	HD11	H	0.921
165	1	17	LEU	HD12	H	0.921
166	1	17	LEU	HD13	H	0.921
167	1	17	LEU	HD21	H	0.885
168	1	17	LEU	HD22	H	0.885
169	1	17	LEU	HD23	H	0.885
170	1	17	LEU	CA	C	57.367
171	1	17	LEU	CB	C	40.980
172	1	17	LEU	CG	C	26.341
173	1	17	LEU	CD1	C	23.280
174	1	17	LEU	CD2	C	23.430
175	1	17	LEU	N	N	119.092
176	1	18	PHE	H	H	8.111
177	1	18	PHE	HA	H	4.229
178	1	18	PHE	HB2	H	3.034
179	1	18	PHE	HB3	H	3.173
180	1	18	PHE	HD1	H	6.877
181	1	18	PHE	HD2	H	6.877
182	1	18	PHE	HE1	H	7.125
183	1	18	PHE	HE2	H	7.125
184	1	18	PHE	CA	C	60.574
185	1	18	PHE	CB	C	38.054
186	1	18	PHE	CD1	C	130.869
187	1	18	PHE	CE1	C	128.793
188	1	18	PHE	N	N	120.232
189	1	19	PHE	H	H	8.297
190	1	19	PHE	HA	H	4.264
191	1	19	PHE	HB2	H	3.290
192	1	19	PHE	HD1	H	7.374
193	1	19	PHE	HD2	H	7.374
194	1	19	PHE	CA	C	61.135
195	1	19	PHE	CB	C	38.169
196	1	19	PHE	CD2	C	130.736
197	1	19	PHE	N	N	119.654
198	1	20	VAL	H	H	8.765
199	1	20	VAL	HA	H	3.730
200	1	20	VAL	HB	H	2.227
201	1	20	VAL	HG11	H	1.135
202	1	20	VAL	HG12	H	1.135
203	1	20	VAL	HG13	H	1.135
204	1	20	VAL	HG21	H	0.951
205	1	20	VAL	HG22	H	0.951
206	1	20	VAL	HG23	H	0.951
207	1	20	VAL	CA	C	65.956
208	1	20	VAL	CB	C	31.203
209	1	20	VAL	CG1	C	21.315
210	1	20	VAL	CG2	C	19.687
211	1	20	VAL	N	N	121.053
212	1	21	GLY	H	H	8.288
213	1	21	GLY	HA2	H	3.774
214	1	21	GLY	HA3	H	3.908
215	1	21	GLY	CA	C	46.380
216	1	21	GLY	N	N	107.186
217	1	22	CYS	H	H	8.000
218	1	22	CYS	HA	H	4.072
219	1	22	CYS	HB2	H	2.637
220	1	22	CYS	HB3	H	2.945
221	1	22	CYS	CA	C	62.586
222	1	22	CYS	CB	C	25.750
223	1	22	CYS	N	N	118.307
224	1	23	GLY	H	H	7.933
225	1	23	GLY	HA2	H	3.783
226	1	23	GLY	HA3	H	3.868
227	1	23	GLY	CA	C	46.377
228	1	23	GLY	N	N	106.906
229	1	24	VAL	H	H	8.163
230	1	24	VAL	HA	H	3.646
231	1	24	VAL	HB	H	2.246
232	1	24	VAL	HG11	H	1.053
233	1	24	VAL	HG12	H	1.053
234	1	24	VAL	HG13	H	1.053
235	1	24	VAL	HG21	H	0.921
236	1	24	VAL	HG22	H	0.921
237	1	24	VAL	HG23	H	0.921
238	1	24	VAL	CA	C	65.984
239	1	24	VAL	CB	C	31.007
240	1	24	VAL	CG1	C	20.921
241	1	24	VAL	CG2	C	19.864
242	1	24	VAL	N	N	122.957
243	1	25	LEU	H	H	7.949
244	1	25	LEU	HA	H	4.064
245	1	25	LEU	HB2	H	1.653
246	1	25	LEU	HB3	H	1.848
247	1	25	LEU	HG	H	1.722
248	1	25	LEU	HD11	H	0.886
249	1	25	LEU	HD12	H	0.886
250	1	25	LEU	HD13	H	0.886
251	1	25	LEU	HD21	H	0.917
252	1	25	LEU	HD22	H	0.917
253	1	25	LEU	HD23	H	0.917
254	1	25	LEU	CA	C	57.724
255	1	25	LEU	CB	C	41.122
256	1	25	LEU	CG	C	26.394
257	1	25	LEU	CD1	C	22.253
258	1	25	LEU	N	N	120.654
259	1	26	LEU	H	H	8.367
260	1	26	LEU	HA	H	4.107
261	1	26	LEU	HB2	H	1.594
262	1	26	LEU	HB3	H	1.861
263	1	26	LEU	HG	H	1.805
264	1	26	LEU	HD11	H	0.889
265	1	26	LEU	HD12	H	0.889
266	1	26	LEU	HD13	H	0.889
267	1	26	LEU	CA	C	57.054
268	1	26	LEU	CB	C	41.167
269	1	26	LEU	CG	C	26.330
270	1	26	LEU	CD1	C	21.923
271	1	26	LEU	N	N	118.188
272	1	27	SER	H	H	7.957
273	1	27	SER	HA	H	4.220
274	1	27	SER	HB2	H	4.033
275	1	27	SER	HB3	H	4.082
276	1	27	SER	CA	C	60.574
277	1	27	SER	CB	C	62.562
278	1	27	SER	N	N	113.765
279	1	28	LYS	H	H	7.861
280	1	28	LYS	HA	H	4.267
281	1	28	LYS	HB2	H	1.960
282	1	28	LYS	HB3	H	1.997
283	1	28	LYS	HG2	H	1.580
284	1	28	LYS	HD2	H	1.684
285	1	28	LYS	HE2	H	2.941
286	1	28	LYS	CA	C	55.720
287	1	28	LYS	CB	C	31.487
288	1	28	LYS	CG	C	23.979
289	1	28	LYS	CD	C	27.970
290	1	28	LYS	CE	C	41.663
291	1	28	LYS	N	N	121.334
292	1	29	LYS	H	H	7.997
293	1	29	LYS	HA	H	4.241
294	1	29	LYS	HB2	H	1.937
295	1	29	LYS	HG2	H	1.555
296	1	29	LYS	HD2	H	1.706
297	1	29	LYS	HE2	H	2.941
298	1	29	LYS	CA	C	56.581
299	1	29	LYS	CB	C	31.816
300	1	29	LYS	CG	C	23.965
301	1	29	LYS	CD	C	28.180
302	1	29	LYS	CE	C	41.663
303	1	29	LYS	N	N	119.512
304	1	30	LYS	H	H	7.913
305	1	30	LYS	HA	H	4.275
306	1	30	LYS	HB2	H	1.854
307	1	30	LYS	HB3	H	1.915
308	1	30	LYS	HG2	H	1.498
309	1	30	LYS	HG3	H	1.532
310	1	30	LYS	HD2	H	1.708
311	1	30	LYS	HE2	H	2.941
312	1	30	LYS	CA	C	56.652
313	1	30	LYS	CB	C	32.284
314	1	30	LYS	CG	C	23.948
315	1	30	LYS	CD	C	28.180
316	1	30	LYS	CE	C	41.663
317	1	30	LYS	N	N	119.596

Release date

2023-05-18

Citation

Permissive Conformations of a Transmembrane Helix Allow Intramembrane Proteolysis by γ-Secretase
Ortner, M., Guschtschin-Schmidt, N., Stelzer, W., Muhle-Goll, C., Langosch, D.
J. Mol. Biol. (2023), 435, 168218-168218, PubMed 37536392 , DOI 10.1016/j.jmb.2023.168218 , Abstract

Related entities 1. Notch 1 extracellular truncation, : 1 : 2 : 9 entities Detail

Experiments performed 4 experiments Detail

#	Name	Isotope labeling	Type	Concentration
1	Notch1 G1740-1743 TMD	natural abundance		500 uM

#	Name	Isotope labeling	Type	Concentration
1	Notch1 G1740-1743 TMD	natural abundance		500 uM

#	Name	Isotope labeling	Type	Concentration
1	Notch1 G1740-1743 TMD	natural abundance		500 uM

#	Name	Isotope labeling	Type	Concentration
1	Notch1 G1740-1743 TMD	natural abundance		500 uM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr34807_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr34807_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	OK	317		100.0 (chain: 1, length: 30)
1	Chemical shift references	chem_shift_reference_1	Warning	2		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 30, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 190
  - ¹³C chemical shifts: 97
  - ¹⁵N chemical shifts: 30
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
5	HIS	HD1	6.719

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	198	159	80.3
¹³C chemical shifts	153	97	63.4
¹⁵N chemical shifts	30	30	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	66	64	97.0
¹³C chemical shifts	60	30	50.0
¹⁵N chemical shifts	30	30	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	132	95	72.0
¹³C chemical shifts	93	67	72.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	15	78.9
¹³C chemical shifts	19	14	73.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	26	16	61.5
¹³C chemical shifts	26	6	23.1

Keywords MEMBRANE PROTEIN, Notch1 G1740-1743, gamma secretase, transmembrane

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Found 1 Document (0.56 seconds)

BMRB: 34807

Related entities 1. Notch 1 extracellular truncation, : 1 : 2 : 9 entities Detail

Experiments performed 4 experiments Detail

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Chemical shift validation 3 contents Detail