BMRB: 34865

Folded alpha helical de novo proteins from Apilactobacillus kunkeei

Authors

Celestine, C.

Assembly

Transposase

Entity

1. Transposase (polymer, Thiol state: not present), 49 monomers, 5848.852 Da Detail

GSMKNYQPNK DLLEILESQK IKIKNLKEEV KILQRKIDQQ IAKERVIKQ

Formula weight

5848.852 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts, spectral_peak_list

Chemcal Shifts

¹H: 262, ¹³C: 96, ¹⁵N: 45 assignments Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val	Val err	Ambig. code
1	1	2	SER	CA	C	55.599	0.000
2	1	2	SER	CB	C	61.238	0.000
3	1	3	MET	H	H	8.505	0.003
4	1	3	MET	HA	H	4.438	0.014
5	1	3	MET	HB2	H	1.966	0.000
6	1	3	MET	CA	C	52.751	0.009
7	1	3	MET	CB	C	30.114	0.000
8	1	3	MET	N	N	122.677	0.078
9	1	4	LYS	H	H	8.295	0.004
10	1	4	LYS	HA	H	4.187	0.007
11	1	4	LYS	HB2	H	1.639	0.007
12	1	4	LYS	HB3	H	1.320	0.000
13	1	4	LYS	CA	C	53.790	0.004
14	1	4	LYS	CB	C	30.183	0.008
15	1	4	LYS	N	N	122.640	0.057
16	1	5	ASN	H	H	8.315	0.005
17	1	5	ASN	HA	H	4.598	0.019
18	1	5	ASN	HB2	H	2.635	0.000
19	1	5	ASN	HB3	H	2.753	0.000
20	1	5	ASN	CA	C	50.413	0.008
21	1	5	ASN	CB	C	36.092	0.000
22	1	5	ASN	N	N	119.342	0.056
23	1	6	TYR	H	H	8.068	0.005
24	1	6	TYR	HA	H	4.460	0.000
25	1	6	TYR	HB2	H	2.908	0.000
26	1	6	TYR	HB3	H	2.906	0.001
27	1	6	TYR	HD1	H	7.030	0.000
28	1	6	TYR	HD2	H	7.030	0.000
29	1	6	TYR	HE1	H	6.753	0.001
30	1	6	TYR	HE2	H	6.753	0.001
31	1	6	TYR	CA	C	55.195	0.005
32	1	6	TYR	CB	C	36.134	0.014
33	1	6	TYR	N	N	121.288	0.098
34	1	7	GLN	H	H	8.148	0.005
35	1	7	GLN	HA	H	4.489	0.007
36	1	7	GLN	CA	C	50.567	0.000
37	1	7	GLN	CB	C	26.791	0.000
38	1	7	GLN	N	N	124.394	0.052
39	1	8	PRO	HA	H	4.249	0.000
40	1	8	PRO	HB2	H	2.198	0.000
41	1	8	PRO	HB3	H	1.800	0.000
42	1	8	PRO	HG2	H	2.764	0.000
43	1	8	PRO	HD2	H	3.529	0.001
44	1	8	PRO	HD3	H	3.394	0.002
45	1	8	PRO	CA	C	60.483	0.000
46	1	8	PRO	CB	C	29.283	0.000
47	1	9	ASN	H	H	8.395	0.005
48	1	9	ASN	HA	H	4.521	0.008
49	1	9	ASN	HD21	H	7.572	0.005
50	1	9	ASN	HD22	H	6.944	0.006
51	1	9	ASN	CA	C	51.230	0.000
52	1	9	ASN	CB	C	35.764	0.000
53	1	9	ASN	N	N	117.810	0.023
54	1	9	ASN	ND2	N	112.545	0.005
55	1	10	LYS	H	H	8.226	0.011
56	1	10	LYS	CA	C	56.389	0.000
57	1	10	LYS	CB	C	30.062	0.000
58	1	10	LYS	N	N	120.318	0.034
59	1	11	ASP	H	H	8.201	0.012
60	1	11	ASP	HA	H	4.366	0.006
61	1	11	ASP	HB2	H	2.635	0.000
62	1	11	ASP	CA	C	53.889	0.015
63	1	11	ASP	CB	C	37.361	0.001
64	1	11	ASP	N	N	118.887	0.033
65	1	12	LEU	H	H	7.708	0.008
66	1	12	LEU	HA	H	4.042	0.007
67	1	12	LEU	HB2	H	1.653	0.000
68	1	12	LEU	HB3	H	1.784	0.000
69	1	12	LEU	HG	H	1.450	0.000
70	1	12	LEU	HD11	H	0.857	0.000
71	1	12	LEU	HD12	H	0.857	0.000
72	1	12	LEU	HD13	H	0.857	0.000
73	1	12	LEU	CA	C	54.933	0.019
74	1	12	LEU	CB	C	39.206	0.054
75	1	12	LEU	N	N	120.622	0.038
76	1	13	LEU	H	H	8.003	0.006
77	1	13	LEU	HA	H	3.976	0.004
78	1	13	LEU	HB2	H	1.818	0.000
79	1	13	LEU	CA	C	55.531	0.000
80	1	13	LEU	CB	C	38.259	0.000
81	1	13	LEU	N	N	118.053	0.042
82	1	14	GLU	H	H	7.809	0.017
83	1	14	GLU	HA	H	4.048	0.003
84	1	14	GLU	HB2	H	2.101	0.000
85	1	14	GLU	HG2	H	2.404	0.000
86	1	14	GLU	CA	C	56.704	0.000
87	1	14	GLU	CB	C	26.550	0.000
88	1	14	GLU	N	N	119.159	0.027
89	1	15	ILE	H	H	7.968	0.008
90	1	15	ILE	HA	H	3.730	0.002
91	1	15	ILE	HB	H	1.948	0.000
92	1	15	ILE	CA	C	61.905	0.000
93	1	15	ILE	CB	C	35.435	0.000
94	1	15	ILE	N	N	121.615	0.047
95	1	16	LEU	H	H	8.272	0.009
96	1	16	LEU	HA	H	4.193	0.007
97	1	16	LEU	HB2	H	1.708	0.000
98	1	16	LEU	HB3	H	1.830	0.000
99	1	16	LEU	HG	H	1.503	0.000
100	1	16	LEU	HD11	H	0.849	0.000
101	1	16	LEU	HD12	H	0.849	0.000
102	1	16	LEU	HD13	H	0.849	0.000
103	1	16	LEU	HD21	H	0.849	0.000
104	1	16	LEU	HD22	H	0.849	0.000
105	1	16	LEU	HD23	H	0.849	0.000
106	1	16	LEU	CA	C	55.757	0.045
107	1	16	LEU	CB	C	39.445	0.005
108	1	16	LEU	N	N	121.848	0.065
109	1	17	GLU	H	H	7.942	0.012
110	1	17	GLU	HA	H	4.036	0.018
111	1	17	GLU	HB2	H	2.055	0.000
112	1	17	GLU	HB3	H	1.953	0.000
113	1	17	GLU	CA	C	56.286	0.022
114	1	17	GLU	CB	C	26.393	0.000
115	1	17	GLU	N	N	118.043	0.050
116	1	18	SER	H	H	8.176	0.010
117	1	18	SER	HA	H	4.201	0.010
118	1	18	SER	HB2	H	3.724	0.000
119	1	18	SER	CA	C	58.838	0.000
120	1	18	SER	CB	C	59.754	0.000
121	1	18	SER	N	N	116.086	0.032
122	1	19	GLN	H	H	8.748	0.004
123	1	19	GLN	HA	H	3.953	0.035
124	1	19	GLN	HB2	H	1.992	0.000
125	1	19	GLN	HB3	H	1.671	0.000
126	1	19	GLN	HG2	H	2.488	0.000
127	1	19	GLN	HG3	H	2.774	0.000
128	1	19	GLN	CA	C	56.738	0.000
129	1	19	GLN	CB	C	26.730	0.000
130	1	19	GLN	N	N	121.241	0.026
131	1	20	LYS	HA	H	4.036	0.000
132	1	20	LYS	HB2	H	2.065	0.000
133	1	20	LYS	CA	C	56.724	0.000
134	1	20	LYS	CB	C	26.605	0.000
135	1	21	ILE	H	H	7.676	0.010
136	1	21	ILE	HA	H	3.719	0.003
137	1	21	ILE	HB	H	1.951	0.000
138	1	21	ILE	HG12	H	1.737	0.000
139	1	21	ILE	HG21	H	1.038	0.000
140	1	21	ILE	HG22	H	1.038	0.000
141	1	21	ILE	HG23	H	1.038	0.000
142	1	21	ILE	HD11	H	0.802	0.000
143	1	21	ILE	HD12	H	0.802	0.000
144	1	21	ILE	HD13	H	0.802	0.000
145	1	21	ILE	CA	C	62.207	0.000
146	1	21	ILE	CB	C	34.962	0.000
147	1	21	ILE	N	N	122.145	0.038
148	1	22	LYS	H	H	7.447	0.017
149	1	22	LYS	HA	H	3.961	0.016
150	1	22	LYS	HB2	H	1.796	0.015
151	1	22	LYS	HB3	H	1.906	0.000
152	1	22	LYS	CA	C	60.267	0.072
153	1	22	LYS	CB	C	29.427	0.056
154	1	22	LYS	N	N	118.938	0.010
155	1	23	ILE	H	H	8.380	0.006
156	1	23	ILE	HA	H	3.389	0.000
157	1	23	ILE	HB	H	1.918	0.000
158	1	23	ILE	HG21	H	0.833	0.000
159	1	23	ILE	HG22	H	0.833	0.000
160	1	23	ILE	HG23	H	0.833	0.000
161	1	23	ILE	HD11	H	0.598	0.000
162	1	23	ILE	HD12	H	0.598	0.000
163	1	23	ILE	HD13	H	0.598	0.000
164	1	23	ILE	CA	C	63.734	0.024
165	1	23	ILE	CB	C	35.765	0.078
166	1	23	ILE	N	N	118.751	0.036
167	1	24	LYS	H	H	7.708	0.013
168	1	24	LYS	HA	H	3.880	0.001
169	1	24	LYS	HB2	H	1.901	0.000
170	1	24	LYS	HB3	H	1.997	0.000
171	1	24	LYS	CA	C	57.452	0.050
172	1	24	LYS	CB	C	29.387	0.052
173	1	24	LYS	N	N	120.070	0.029
174	1	25	ASN	H	H	8.452	0.009
175	1	25	ASN	HA	H	4.472	0.004
176	1	25	ASN	HB2	H	2.871	0.005
177	1	25	ASN	HB3	H	2.866	0.000
178	1	25	ASN	HD21	H	6.852	0.000
179	1	25	ASN	HD22	H	7.483	0.000
180	1	25	ASN	CA	C	53.286	0.003
181	1	25	ASN	CB	C	35.124	0.068
182	1	25	ASN	N	N	118.080	0.033
183	1	26	LEU	H	H	8.646	0.010
184	1	26	LEU	HA	H	4.134	0.007
185	1	26	LEU	HB2	H	2.029	0.000
186	1	26	LEU	HB3	H	1.794	0.000
187	1	26	LEU	HG	H	1.567	0.000
188	1	26	LEU	HD11	H	0.862	0.000
189	1	26	LEU	HD12	H	0.862	0.000
190	1	26	LEU	HD13	H	0.862	0.000
191	1	26	LEU	CA	C	55.580	0.067
192	1	26	LEU	CB	C	41.104	0.090
193	1	26	LEU	N	N	123.612	0.025
194	1	27	LYS	H	H	8.612	0.010
195	1	27	LYS	HA	H	3.830	0.022
196	1	27	LYS	HB2	H	1.942	0.021
197	1	27	LYS	HB3	H	1.772	0.003
198	1	27	LYS	HG2	H	1.366	0.000
199	1	27	LYS	HD2	H	1.612	0.000
200	1	27	LYS	CA	C	57.837	0.046
201	1	27	LYS	CB	C	29.606	0.055
202	1	27	LYS	N	N	118.465	0.038
203	1	28	GLU	H	H	7.709	0.015
204	1	28	GLU	HA	H	4.060	0.008
205	1	28	GLU	HB2	H	1.642	0.000
206	1	28	GLU	HB3	H	1.401	0.000
207	1	28	GLU	HG2	H	2.129	0.000
208	1	28	GLU	HG3	H	2.366	0.000
209	1	28	GLU	CA	C	56.346	0.046
210	1	28	GLU	CB	C	26.259	0.000
211	1	28	GLU	N	N	119.467	0.081
212	1	29	GLU	H	H	7.962	0.009
213	1	29	GLU	HA	H	3.964	0.000
214	1	29	GLU	HB2	H	2.150	0.000
215	1	29	GLU	HG2	H	2.151	0.000
216	1	29	GLU	HG3	H	2.352	0.000
217	1	29	GLU	CA	C	57.528	0.046
218	1	29	GLU	CB	C	26.888	0.079
219	1	29	GLU	N	N	121.620	0.054
220	1	30	VAL	H	H	8.241	0.010
221	1	30	VAL	HA	H	3.378	0.009
222	1	30	VAL	HB	H	2.039	0.000
223	1	30	VAL	HG11	H	0.971	0.000
224	1	30	VAL	HG12	H	0.971	0.000
225	1	30	VAL	HG13	H	0.971	0.000
226	1	30	VAL	HG21	H	0.840	0.002
227	1	30	VAL	HG22	H	0.840	0.002
228	1	30	VAL	HG23	H	0.840	0.002
229	1	30	VAL	CA	C	64.768	0.001
230	1	30	VAL	CB	C	29.242	0.000
231	1	30	VAL	N	N	118.253	0.043
232	1	31	LYS	H	H	7.391	0.013
233	1	31	LYS	HA	H	3.959	0.008
234	1	31	LYS	HB2	H	1.367	0.000
235	1	31	LYS	HB3	H	1.936	0.000
236	1	31	LYS	CA	C	57.014	0.004
237	1	31	LYS	CB	C	29.567	0.000
238	1	31	LYS	N	N	118.387	0.044
239	1	32	ILE	H	H	8.096	0.012
240	1	32	ILE	HA	H	4.464	0.000
241	1	32	ILE	HB	H	1.712	0.000
242	1	32	ILE	HG21	H	1.134	0.000
243	1	32	ILE	HG22	H	1.134	0.000
244	1	32	ILE	HG23	H	1.134	0.000
245	1	32	ILE	HD11	H	0.851	0.000
246	1	32	ILE	HD12	H	0.851	0.000
247	1	32	ILE	HD13	H	0.851	0.000
248	1	32	ILE	CA	C	62.321	0.000
249	1	32	ILE	CB	C	35.485	0.000
250	1	32	ILE	N	N	120.515	0.044
251	1	33	LEU	HA	H	3.962	0.000
252	1	33	LEU	HD11	H	0.848	0.000
253	1	33	LEU	HD12	H	0.848	0.000
254	1	33	LEU	HD13	H	0.848	0.000
255	1	33	LEU	HD21	H	0.973	0.000
256	1	33	LEU	HD22	H	0.973	0.000
257	1	33	LEU	HD23	H	0.973	0.000
258	1	33	LEU	CA	C	55.404	0.000
259	1	33	LEU	CB	C	39.604	0.000
260	1	34	GLN	H	H	8.816	0.004
261	1	34	GLN	HA	H	3.859	0.009
262	1	34	GLN	HB2	H	1.955	0.023
263	1	34	GLN	HB3	H	2.229	0.000
264	1	34	GLN	HG2	H	2.449	0.000
265	1	34	GLN	HG3	H	2.242	0.000
266	1	34	GLN	CA	C	55.917	0.078
267	1	34	GLN	CB	C	25.497	0.013
268	1	34	GLN	N	N	118.438	0.027
269	1	35	ARG	H	H	7.853	0.007
270	1	35	ARG	HA	H	4.116	0.015
271	1	35	ARG	CA	C	56.472	0.000
272	1	35	ARG	CB	C	27.035	0.000
273	1	35	ARG	N	N	118.566	0.029
274	1	36	LYS	HA	H	3.809	0.000
275	1	36	LYS	HB2	H	1.954	0.000
276	1	36	LYS	CA	C	58.062	0.000
277	1	36	LYS	CB	C	29.823	0.000
278	1	37	ILE	H	H	7.577	0.013
279	1	37	ILE	HA	H	3.745	0.000
280	1	37	ILE	HB	H	1.916	0.000
281	1	37	ILE	HG12	H	1.501	0.000
282	1	37	ILE	HG21	H	1.225	0.000
283	1	37	ILE	HG22	H	1.225	0.000
284	1	37	ILE	HG23	H	1.225	0.000
285	1	37	ILE	HD11	H	0.858	0.000
286	1	37	ILE	HD12	H	0.858	0.000
287	1	37	ILE	HD13	H	0.858	0.000
288	1	37	ILE	CA	C	62.008	0.000
289	1	37	ILE	CB	C	35.163	0.000
290	1	37	ILE	N	N	120.676	0.037
291	1	38	ASP	H	H	8.424	0.009
292	1	38	ASP	HA	H	4.366	0.001
293	1	38	ASP	HB2	H	2.753	0.002
294	1	38	ASP	HB3	H	2.546	0.000
295	1	38	ASP	CA	C	54.478	0.068
296	1	38	ASP	CB	C	37.544	0.021
297	1	38	ASP	N	N	120.984	0.024
298	1	39	GLN	H	H	8.106	0.004
299	1	39	GLN	HA	H	4.086	0.010
300	1	39	GLN	HB2	H	2.102	0.000
301	1	39	GLN	HB3	H	2.174	0.000
302	1	39	GLN	HG2	H	2.357	0.000
303	1	39	GLN	HG3	H	2.153	0.000
304	1	39	GLN	HE21	H	7.495	0.004
305	1	39	GLN	HE22	H	6.461	0.013
306	1	39	GLN	CA	C	55.438	0.006
307	1	39	GLN	CB	C	25.853	0.072
308	1	39	GLN	N	N	118.736	0.068
309	1	39	GLN	NE2	N	113.433	0.010
310	1	40	GLN	H	H	7.838	0.019
311	1	40	GLN	HA	H	4.054	0.000
312	1	40	GLN	HB2	H	2.170	0.000
313	1	40	GLN	HB3	H	2.096	0.000
314	1	40	GLN	HG2	H	2.335	0.000
315	1	40	GLN	HG3	H	2.393	0.000
316	1	40	GLN	HE21	H	6.631	0.000
317	1	40	GLN	CA	C	55.166	0.028
318	1	40	GLN	CB	C	25.593	0.027
319	1	40	GLN	N	N	119.914	0.067
320	1	41	ILE	H	H	8.043	0.002
321	1	41	ILE	HA	H	3.846	0.021
322	1	41	ILE	HB	H	1.867	0.000
323	1	41	ILE	HG12	H	1.404	0.000
324	1	41	ILE	HG21	H	1.152	0.000
325	1	41	ILE	HG22	H	1.152	0.000
326	1	41	ILE	HG23	H	1.152	0.000
327	1	41	ILE	HD11	H	0.838	0.000
328	1	41	ILE	HD12	H	0.838	0.000
329	1	41	ILE	HD13	H	0.838	0.000
330	1	41	ILE	CA	C	60.742	0.012
331	1	41	ILE	CB	C	35.475	0.006
332	1	41	ILE	N	N	119.991	0.047
333	1	42	ALA	H	H	7.866	0.010
334	1	42	ALA	HA	H	4.114	0.007
335	1	42	ALA	HB1	H	1.389	0.001
336	1	42	ALA	HB2	H	1.389	0.001
337	1	42	ALA	HB3	H	1.389	0.001
338	1	42	ALA	CA	C	51.091	0.009
339	1	42	ALA	CB	C	15.743	0.002
340	1	42	ALA	N	N	123.458	0.132
341	1	43	LYS	H	H	7.771	0.012
342	1	43	LYS	HA	H	4.116	0.022
343	1	43	LYS	HB2	H	1.835	0.005
344	1	43	LYS	HB3	H	1.634	0.000
345	1	43	LYS	CA	C	54.787	0.017
346	1	43	LYS	CB	C	29.960	0.004
347	1	43	LYS	N	N	118.558	0.032
348	1	44	GLU	H	H	7.929	0.017
349	1	44	GLU	HA	H	4.140	0.006
350	1	44	GLU	HB2	H	1.983	0.001
351	1	44	GLU	HG2	H	2.197	0.000
352	1	44	GLU	HG3	H	2.317	0.000
353	1	44	GLU	CA	C	54.425	0.003
354	1	44	GLU	CB	C	27.321	0.080
355	1	44	GLU	N	N	119.538	0.058
356	1	45	ARG	H	H	7.930	0.015
357	1	45	ARG	HA	H	4.233	0.007
358	1	45	ARG	HB2	H	1.770	0.005
359	1	45	ARG	HB3	H	1.575	0.017
360	1	45	ARG	CA	C	53.904	0.048
361	1	45	ARG	CB	C	27.924	0.025
362	1	45	ARG	N	N	120.439	0.038
363	1	46	VAL	H	H	7.901	0.007
364	1	46	VAL	HA	H	4.009	0.010
365	1	46	VAL	HB	H	2.004	0.000
366	1	46	VAL	HG11	H	0.891	0.000
367	1	46	VAL	HG12	H	0.891	0.000
368	1	46	VAL	HG13	H	0.891	0.000
369	1	46	VAL	HG21	H	0.891	0.000
370	1	46	VAL	HG22	H	0.891	0.000
371	1	46	VAL	HG23	H	0.891	0.000
372	1	46	VAL	CA	C	59.900	0.002
373	1	46	VAL	CB	C	29.812	0.002
374	1	46	VAL	N	N	121.182	0.049
375	1	47	ILE	H	H	8.139	0.003
376	1	47	ILE	HA	H	4.081	0.010
377	1	47	ILE	HB	H	1.783	0.001
378	1	47	ILE	HG12	H	1.128	0.003
379	1	47	ILE	HG21	H	1.417	0.000
380	1	47	ILE	HG22	H	1.417	0.000
381	1	47	ILE	HG23	H	1.417	0.000
382	1	47	ILE	HD11	H	0.840	0.000
383	1	47	ILE	HD12	H	0.840	0.000
384	1	47	ILE	HD13	H	0.840	0.000
385	1	47	ILE	CA	C	58.228	0.003
386	1	47	ILE	CB	C	35.782	0.007
387	1	47	ILE	N	N	125.268	0.050
388	1	48	LYS	H	H	8.281	0.005
389	1	48	LYS	HA	H	4.282	0.007
390	1	48	LYS	HB2	H	1.841	0.000
391	1	48	LYS	HB3	H	1.675	0.001
392	1	48	LYS	HG2	H	1.381	0.000
393	1	48	LYS	HG3	H	1.381	0.000
394	1	48	LYS	CA	C	53.533	0.006
395	1	48	LYS	CB	C	30.332	0.001
396	1	48	LYS	N	N	126.691	0.035
397	1	49	GLN	H	H	7.984	0.008
398	1	49	GLN	HA	H	4.091	0.008
399	1	49	GLN	HB2	H	2.011	0.000
400	1	49	GLN	HG2	H	2.227	0.000
401	1	49	GLN	CA	C	54.619	0.000
402	1	49	GLN	CB	C	27.669	0.000
403	1	49	GLN	N	N	127.820	0.019

Release date

2023-12-14

Citation

Folded Alpha Helical Putative New Proteins from Apilactobacillus kunkeei
Ye, W., Krishna Behra, P.R., Dyrhage, K., Seeger, C., Joiner, J.D., Karlsson, E., Andersson, E., Chi, C.N., Andersson, S., Jemth, P.
J. Mol. Biol. (2024), 436, 168490-168490, PubMed 38355092 , DOI 10.1016/j.jmb.2024.168490 , Abstract

Entries sharing articles BMRB: 2 entries Detail

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Experiments performed 7 experiments Detail

Chemical shift validation 3 contents Detail