BMRB: 36017

Solution NMR structure of a new lasso peptide sphaericin

Authors

Hemmi, H., Kodani, S., Inoue, Y., Suzuki, M., Dohra, H., Suzuki, T., Ohnishi-Kameyama, M.

Assembly

Uncharacterized protein

Entity

1. Uncharacterized protein (polymer, Thiol state: not present), 18 monomers, 2174.499 Da Detail

GLPIGWWIER PSGWYFPI

Formula weight

2174.499 Da

Source organism

Planomonospora sphaerica

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.3 %, Completeness (bb): 80.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	92.3 % (203 of 220)	100.0 % (124 of 124)	82.3 % (79 of 96)
Backbone	80.5 % (70 of 87)	100.0 % (36 of 36)	66.7 % (34 of 51)
Sidechain	100.0 % (148 of 148)	100.0 % (88 of 88)	100.0 % (60 of 60)
Aromatic	100.0 % (51 of 51)	100.0 % (27 of 27)	100.0 % (24 of 24)
Methyl	100.0 % (16 of 16)	100.0 % (8 of 8)	100.0 % (8 of 8)

1. entity 1

GLPIGWWIER PSGWYFPI

Show sample condition

Sample

Solvent system DMSO, Pressure 1 atm, Temperature 298 K, Details 10 mg/mL NA-C,H peptide, DMSO

#	Name	Isotope labeling	Type	Concentration
1	peptide	NA-C,H	protein	10 mg/mL
2	DMSO-d6	natural abundance		100 %

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Protein Blocks Logo

Calculated from 15 models in PDB: 5GVO, Strand ID: A Detail

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Release date

2017-06-07

Citation

Sphaericin, a Lasso Peptide from the Rare Actinomycete Planomonospora sphaerica
Kodani, S., Inoue, Y., Suzuki, M., Dohra, H., Suzuki, T., Hemmi, H., Ohnishi-Kameyama, M.
Eur. J. Org. Chem. (2017), 2017, 1177-1183, PubMed , DOI 10.1002/ejoc.201601334

Related entities 1. Uncharacterized protein, : 1 entities Detail

Experiments performed 7 experiments Detail