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BMRB: 36021

5GWM

Solution structure of heterodimeric coiled-coil domain of Drosophila GABAB receptor 1 and 3

Authors

Liu, X., Zhang, S., Zhang, C.X., Liu, J.

Assembly

Metabotropic GABA-B receptor subtype 1, Metabotropic GABA-B receptor subtype 3, isoform A

Entity

1. Metabotropic GABA-B receptor subtype 1, Metabotropic GABA-B receptor subtype 3, isoform A, entity 1 (polymer, Thiol state: not present), 53 monomers, 6316.031 Da Detail

MDSAISKEDE ERYQKLVTEN EQLQRLITQK EEKIRVLRQR LVERGDAKGT ELN

2. Metabotropic GABA-B receptor subtype 1, Metabotropic GABA-B receptor subtype 3, isoform A, entity 2 (polymer, Thiol state: not present), 50 monomers, 5943.730 Da Detail

GPLGSRRFVV DDRRELQYRV EVQNRVYKKE IQALDAEIRK LERLLESGLT

Total weight

12259.761 Da

Max. entity weight

6316.031 Da

Source organism

Drosophila melanogaster

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 88.7 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.7 % (1118 of 1261)	88.4 % (596 of 674)	86.9 % (412 of 474)	97.3 % (110 of 113)
Backbone	96.6 % (595 of 616)	97.6 % (205 of 210)	95.4 % (290 of 304)	98.0 % (100 of 102)
Sidechain	83.0 % (617 of 743)	84.3 % (391 of 464)	80.6 % (216 of 268)	90.9 % (10 of 11)
Aromatic	50.0 % (17 of 34)	100.0 % (17 of 17)	0.0 % (0 of 17)
Methyl	95.0 % (114 of 120)	98.3 % (59 of 60)	91.7 % (55 of 60)

1. entity 1

MDSAISKEDE ERYQKLVTEN EQLQRLITQK EEKIRVLRQR LVERGDAKGT ELN

2. entity 2

GPLGSRRFVV DDRRELQYRV EVQNRVYKKE IQALDAEIRK LERLLESGLT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0, Details 20 mM PBS, 100 mM sodium chloride, 0.02 mg/mL sodium azide, 0.7 mM [U-99% 15N] Molecule1, 0.7 mM [U-99% 15N] Molecule2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Molecule1	[U-99% 15N]	protein	0.7 mM
2	Molecule2	[U-99% 15N]	protein	0.7 mM
3	PBS	natural abundance	buffer	20 mM
4	sodium azide	natural abundance		0.02 mg/mL
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0, Details 20 mM PBS, 100 mM sodium chloride, 0.02 mg/mL sodium azide, 0.7 mM [U-99% 13C; U-99% 15N] Molecule1, 0.7 mM [U-99% 13C; U-99% 15N] Molecule2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
8	Molecule1	[U-99% 13C; U-99% 15N]	protein	0.7 mM
9	Molecule2	[U-99% 13C; U-99% 15N]	protein	0.7 mM
10	PBS	natural abundance	buffer	20 mM
11	sodium azide	natural abundance		0.02 mg/mL
12	sodium chloride	natural abundance	salt	100 mM
13	H2O	natural abundance	solvent	90 %
14	D2O	[U-2H]	solvent	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 5GWM, Strand ID: A, B Detail

Release date

2016-10-27

Citation

Solution structure of heterodimeric coiled-coil domain of Drosophila GABAB receptor 1 and 3
Liu, X., Zhang, S., Zhang, C.X., Liu, J.

Related entities 1. Metabotropic GABA-B receptor subtype 1, Metabotropic GABA-B receptor subtype 3, isoform A, entity 1, : 5 entities Detail

Related entities 2. Metabotropic GABA-B receptor subtype 1, Metabotropic GABA-B receptor subtype 3, isoform A, entity 2, : 5 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Agilent DD2 - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 7.0, Details 20 mM PBS, 100 mM sodium chloride, 0.02 mg/mL sodium azide, 0.7 mM [U-99% 15N] Molecule1, 0.7 mM [U-99% 15N] Molecule2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Molecule1	[U-99% 15N]	protein	0.7 mM
2	Molecule2	[U-99% 15N]	protein	0.7 mM
3	PBS	natural abundance	buffer	20 mM
4	sodium azide	natural abundance		0.02 mg/mL
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

2 3D 1H-15N NOESY

Instrument

Agilent DD2 - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Molecule1	[U-99% 15N]	protein	0.7 mM
2	Molecule2	[U-99% 15N]	protein	0.7 mM
3	PBS	natural abundance	buffer	20 mM
4	sodium azide	natural abundance		0.02 mg/mL
5	sodium chloride	natural abundance	salt	100 mM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

3 2D 1H-15N HSQC