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BMRB: 36037

5WQZ

Solution structure of a histone binding domain

Authors

Assembly

Putative uncharacterized protein

Entity

1. Putative uncharacterized protein (polymer, Thiol state: all free), 124 monomers, 13864.60 Da Detail

MSQNRQLLYP REEMVSLVRS LDRPQENGLF SQDVLLQYPE LAESYTKVCP NRCDLATAAD RAAKGAYGYD VQLTTLKEDI RLMVNNCILF NGAEGAYADA ARTFEKFAMG KIDAYISQKV GGLE

Formula weight

13864.6 Da

Source organism

Trypanosoma brucei brucei TREU927

Exptl. method

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 71.1 %, Completeness (bb): 82.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	71.1 % (1013 of 1425)	73.0 % (541 of 741)	62.6 % (345 of 551)	95.5 % (127 of 133)
Backbone	82.3 % (606 of 736)	95.6 % (241 of 252)	68.1 % (248 of 364)	97.5 % (117 of 120)
Sidechain	64.5 % (519 of 805)	61.3 % (300 of 489)	69.0 % (209 of 303)	76.9 % (10 of 13)
Aromatic	16.7 % (16 of 96)	33.3 % (16 of 48)	0.0 % (0 of 48)
Methyl	77.5 % (107 of 138)	76.8 % (53 of 69)	78.3 % (54 of 69)

1. entity 1

MSQNRQLLYP REEMVSLVRS LDRPQENGLF SQDVLLQYPE LAESYTKVCP NRCDLATAAD RAAKGAYGYD VQLTTLKEDI RLMVNNCILF NGAEGAYADA ARTFEKFAMG KIDAYISQKV GGLE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 g/L [U-100% 15N] NH4Cl, 2.5 g/L [U-100% 13C] C6H12O6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C, U-15N]	protein	0.0 ~ 0.0 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.18 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5WQZ, Strand ID: A Detail

Release date

2017-03-06

Citation

Solution Structure Of A Histone Binding Domain
Mi, J.

Related entities 1. Putative uncharacterized protein, : 1 : 2 : 11 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 g/L [U-100% 15N] NH4Cl, 2.5 g/L [U-100% 13C] C6H12O6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C, U-15N]	protein	0.0 ~ 0.0 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

2 3D 1H-15N NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 g/L [U-100% 15N] NH4Cl, 2.5 g/L [U-100% 13C] C6H12O6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C, U-15N]	protein	0.0 ~ 0.0 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

3 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 g/L [U-100% 15N] NH4Cl, 2.5 g/L [U-100% 13C] C6H12O6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C, U-15N]	protein	0.0 ~ 0.0 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

4 3D HBHA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 g/L [U-100% 15N] NH4Cl, 2.5 g/L [U-100% 13C] C6H12O6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C, U-15N]	protein	0.0 ~ 0.0 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

5 3D H(CCO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 g/L [U-100% 15N] NH4Cl, 2.5 g/L [U-100% 13C] C6H12O6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C, U-15N]	protein	0.0 ~ 0.0 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

6 3D 1H-13C NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 0.5 g/L [U-100% 15N] NH4Cl, 2.5 g/L [U-100% 13C] C6H12O6, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	entity_1	[U-13C, U-15N]	protein	0.0 ~ 0.0 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

nullKeywords HISTONE BINDING DOMAIN, PROTEIN BINDING