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Found 1 Document (1.133 seconds)

BMRB: 36049

5X29

NMR structure of the SARS Coronavirus E protein pentameric ion channel

Authors

Torres, J., Surya, W., Li, Y.

Assembly

Envelope small membrane protein

Entity

1. Envelope small membrane protein (polymer, Thiol state: not present), 81 monomers, 8993.454 × 5 Da Detail

MHHHHHHSSG VDLGTENLYF QSMETGTLIV NSVLLFLAFV VFLLVTLAIL TALRLAAYAA NIVNVSLVKP TVYVYSRVKN L

Total weight

44967.27 Da

Max. entity weight

8993.454 Da

Source organism

Severe acute respiratory syndrome-related coronavirus

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 77.8 %, Completeness: 73.0 %, Completeness (bb): 74.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.0 % (692 of 948)	71.5 % (339 of 474)	74.0 % (287 of 388)	76.7 % (66 of 86)
Backbone	74.8 % (362 of 484)	73.2 % (120 of 164)	75.0 % (180 of 240)	77.5 % (62 of 80)
Sidechain	72.0 % (390 of 542)	70.6 % (219 of 310)	73.9 % (167 of 226)	66.7 % (4 of 6)
Aromatic	47.9 % (46 of 96)	50.0 % (24 of 48)	45.8 % (22 of 48)
Methyl	89.4 % (127 of 142)	88.7 % (63 of 71)	90.1 % (64 of 71)

1. Envelope small membrane protein

MHHHHHHSSG VDLGTENLYF QSMETGTLIV NSVLLFLAFV VFLLVTLAIL TALRLAAYAA NIVNVSLVKP TVYVYSRVKN L

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5, Details 0.67 mM [U-99% 15N] Envelope small membrane protein, 200 mM LMPG, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Envelope small membrane protein	[U-99% 15N]	protein	0.67 mM
2	LMPG	natural abundance		200 mM
3	sodium chloride	natural abundance	salt	50 mM
4	sodium phosphate	natural abundance	buffer	20 mM
5	H2O	natural abundance	solvent	95 %
6	D2O	[U-2H]	solvent	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5, Details 0.67 mM [U-99% 13C; U-99% 15N] Envelope small membrane protein, 200 mM LMPG, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
7	Envelope small membrane protein	[U-99% 13C; U-99% 15N]	protein	0.67 mM
8	LMPG	natural abundance		200 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium phosphate	natural abundance	buffer	20 mM
11	H2O	natural abundance	solvent	95 %
12	D2O	[U-2H]	solvent	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5, Details 0.67 mM [U-99% 15N, U-99% 2H] Envelope small membrane protein, 200 mM LMPG, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
13	Envelope small membrane protein	[U-99% 15N, U-99% 2H]	protein	0.67 mM
14	LMPG	natural abundance		200 mM
15	sodium chloride	natural abundance	salt	50 mM
16	sodium phosphate	natural abundance	buffer	20 mM
17	H2O	natural abundance	solvent	95 %
18	D2O	[U-2H]	solvent	5 %

Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5, Details 0.33 mM [U-99% 13C] Envelope small membrane protein, 0.33 mM [U-99% 15N, U-99% 2H] Envelope small membrane protein, 200 mM LMPG, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
19	Envelope small membrane protein	[U-99% 13C]	protein	0.33 mM
20	Envelope small membrane protein	[U-99% 15N, U-99% 2H]	protein	0.33 mM
21	LMPG	natural abundance		200 mM
22	sodium chloride	natural abundance	salt	50 mM
23	sodium phosphate	natural abundance	buffer	20 mM
24	H2O	natural abundance	solvent	95 %
25	D2O	[U-2H]	solvent	5 %

Protein Blocks Logo

Calculated from 16 models in PDB: 5X29, Strand ID: A, B, C, D, E Detail

Release date

2017-02-28

Citation

Structural model of the SARS coronavirus E channel in LMPG micelles
Surya, W., Li, Y., Torres, J.
Biochim. Biophys. Acta. Biomembr. (2018), 1860, 1309-1317, PubMed 29474890 , DOI 10.1016/j.bbamem.2018.02.017 , Abstract

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5, Details 0.67 mM [U-99% 15N] Envelope small membrane protein, 200 mM LMPG, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Envelope small membrane protein	[U-99% 15N]	protein	0.67 mM
2	LMPG	natural abundance		200 mM
3	sodium chloride	natural abundance	salt	50 mM
4	sodium phosphate	natural abundance	buffer	20 mM
5	H2O	natural abundance	solvent	95 %
6	D2O	[U-2H]	solvent	5 %

2 3D HNCA

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5, Details 0.67 mM [U-99% 13C; U-99% 15N] Envelope small membrane protein, 200 mM LMPG, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
7	Envelope small membrane protein	[U-99% 13C; U-99% 15N]	protein	0.67 mM
8	LMPG	natural abundance		200 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium phosphate	natural abundance	buffer	20 mM
11	H2O	natural abundance	solvent	95 %
12	D2O	[U-2H]	solvent	5 %

3 3D HN(CO)CA

Instrument

Bruker AvanceII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
7	Envelope small membrane protein	[U-99% 13C; U-99% 15N]	protein	0.67 mM
8	LMPG	natural abundance		200 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium phosphate	natural abundance	buffer	20 mM
11	H2O	natural abundance	solvent	95 %
12	D2O	[U-2H]	solvent	5 %

4 3D HCCH-TOCSY

Instrument

Bruker AvanceII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
7	Envelope small membrane protein	[U-99% 13C; U-99% 15N]	protein	0.67 mM
8	LMPG	natural abundance		200 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium phosphate	natural abundance	buffer	20 mM
11	H2O	natural abundance	solvent	95 %
12	D2O	[U-2H]	solvent	5 %

5 3D 1H-15N NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
7	Envelope small membrane protein	[U-99% 13C; U-99% 15N]	protein	0.67 mM
8	LMPG	natural abundance		200 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium phosphate	natural abundance	buffer	20 mM
11	H2O	natural abundance	solvent	95 %
12	D2O	[U-2H]	solvent	5 %

6 3D 1H-13C NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
7	Envelope small membrane protein	[U-99% 13C; U-99% 15N]	protein	0.67 mM
8	LMPG	natural abundance		200 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium phosphate	natural abundance	buffer	20 mM
11	H2O	natural abundance	solvent	95 %
12	D2O	[U-2H]	solvent	5 %

7 2D 1H-13C HSQC

Instrument

Bruker AvanceII - 700 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
7	Envelope small membrane protein	[U-99% 13C; U-99% 15N]	protein	0.67 mM
8	LMPG	natural abundance		200 mM
9	sodium chloride	natural abundance	salt	50 mM
10	sodium phosphate	natural abundance	buffer	20 mM
11	H2O	natural abundance	solvent	95 %
12	D2O	[U-2H]	solvent	5 %

8 3D 1H-15N NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5, Details 0.67 mM [U-99% 15N, U-99% 2H] Envelope small membrane protein, 200 mM LMPG, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
13	Envelope small membrane protein	[U-99% 15N, U-99% 2H]	protein	0.67 mM
14	LMPG	natural abundance		200 mM
15	sodium chloride	natural abundance	salt	50 mM
16	sodium phosphate	natural abundance	buffer	20 mM
17	H2O	natural abundance	solvent	95 %
18	D2O	[U-2H]	solvent	5 %

9 3D 1H-15N NOESY

Instrument

Bruker AvanceII - 700 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 5.5, Details 0.33 mM [U-99% 13C] Envelope small membrane protein, 0.33 mM [U-99% 15N, U-99% 2H] Envelope small membrane protein, 200 mM LMPG, 20 mM sodium phosphate, 50 mM sodium chloride, 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
19	Envelope small membrane protein	[U-99% 13C]	protein	0.33 mM
20	Envelope small membrane protein	[U-99% 15N, U-99% 2H]	protein	0.33 mM
21	LMPG	natural abundance		200 mM
22	sodium chloride	natural abundance	salt	50 mM
23	sodium phosphate	natural abundance	buffer	20 mM
24	H2O	natural abundance	solvent	95 %
25	D2O	[U-2H]	solvent	5 %

nullKeywords envelope protein, ion channel, membrane protein, pentamer, viral protein

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Found 1 Document (1.133 seconds)

BMRB: 36049

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Experiments performed 9 experiments Detail

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