BMRB: 36062

N-terminal Zinc Finger of Synaptotagmin-like Protein 4

Authors

Miyamoto, K.

Assembly

Synaptotagmin-like protein 4

Entity

1. Synaptotagmin-like protein 4 (polymer, Thiol state: all other bound), 53 monomers, 6029.876 Da Detail

SDRTCARCQE SLGRLSPKTN TCRGCNHLVC RDCRIQESNG TWRCKVCAKE IEL

2. ZINC ION (non-polymer), 65.409 × 2 Da

Total weight

6160.694 Da

Max. entity weight

6029.876 Da

Entity Connection

metal coordination 8 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS5:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS8:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS22:SG	2:ZN1:ZN
4	metal coordination	sing	1:CYS25:SG	2:ZN1:ZN
5	metal coordination	sing	1:CYS30:SG	2:ZN1:ZN
6	metal coordination	sing	1:CYS33:SG	2:ZN1:ZN
7	metal coordination	sing	1:CYS44:SG	2:ZN1:ZN
8	metal coordination	sing	1:CYS47:SG	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 18.5 %, Completeness (bb): 50.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	18.5 % (58 of 313)	18.5 % (58 of 313)
Backbone	50.0 % (54 of 108)	50.0 % (54 of 108)
Sidechain	2.0 % (4 of 205)	2.0 % (4 of 205)
Aromatic	0.0 % (0 of 8)	0.0 % (0 of 8)
Methyl	0.0 % (0 of 22)	0.0 % (0 of 22)

1. Synaptotagmin-like protein 4

SDRTCARCQE SLGRLSPKTN TCRGCNHLVC RDCRIQESNG TWRCKVCAKE IEL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.9, Details 20 mM d11 Tris-HCl, 50 mM NaCl, 1 mM d10 1,4-DL-dithiothreitol, 50 uM ZnCl2, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Synaptotagmin-like Protein 4	natural abundance	protein	1 mM
2	1,4-DL-dithiothreitol	[U-2H]		1 mM
3	NaCl	natural abundance	salt	50 mM
4	Tris-HCl	[U-2H]	buffer	20 mM
5	ZnCl2	natural abundance		50 uM
6	H2O	natural abundance	solvent	90 %
7	D2O	[U-2H]	solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 5X6T, Strand ID: A Detail

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Release date

2017-10-22

Citation

The unique N-terminal zinc finger of Synaptotagmin-like protein 4 reveals FYVE structure
Miyamoto, K., Nakatani, A., Saito, K.
Protein Sci. (2017), 26, 2451-2457, PubMed 28906046 , DOI 10.1002/pro.3301 , Abstract

Related entities 1. Synaptotagmin-like protein 4, : 1 : 2 : 92 entities Detail

Interaction partners 1. Synaptotagmin-like protein 4, : 8 interactors Detail

Experiments performed 1 experiments Detail