BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	30.8 % (253 of 821)	31.6 % (137 of 433)	18.7 % (60 of 321)	83.6 % (56 of 67)
Backbone	56.1 % (213 of 380)	76.7 % (99 of 129)	30.4 % (58 of 191)	93.3 % (56 of 60)
Sidechain	8.4 % (42 of 502)	12.5 % (38 of 304)	2.1 % (4 of 191)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 94)	0.0 % (0 of 47)	0.0 % (0 of 47)
Methyl	13.3 % (8 of 60)	20.0 % (6 of 30)	6.7 % (2 of 30)

1. Spike protein S2

PRNTREVFAQ VKQMYKTPTL KYFGGFNFSQ ILPSPLKPTK RSFIEDLLFN KVTLADAGFM KQYGE

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Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.2 M [U-13C; U-15N] Large Fragment of the SARS-CoV Fusion Protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Large Fragment of the SARS-CoV Fusion Protein	[U-13C; U-15N]	protein	0.2 M
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

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Calculated from 20 models in PDB: 5XJK, Strand ID: A Detail

Release date

2017-10-22

Citation

NMR structure and localization of a large fragment of the SARS-CoV fusion protein: Implications in viral cell fusion
Mahajan, M., Chatterjee, D., Bhuvaneswari, K., Pillay, S., Bhattacharjya, S.
Biochim. Biophys. Acta (2018), 1860, 407-415, PubMed 28988778 , DOI 10.1016/j.bbamem.2017.10.002 , Abstract

Related entities 1. Spike protein S2, : 1 : 35 : 71 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.2 M [U-13C; U-15N] Large Fragment of the SARS-CoV Fusion Protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Large Fragment of the SARS-CoV Fusion Protein	[U-13C; U-15N]	protein	0.2 M
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

2 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Large Fragment of the SARS-CoV Fusion Protein	[U-13C; U-15N]	protein	0.2 M
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

3 3D HNCA

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Large Fragment of the SARS-CoV Fusion Protein	[U-13C; U-15N]	protein	0.2 M
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

4 3D HN(CO)CA

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Large Fragment of the SARS-CoV Fusion Protein	[U-13C; U-15N]	protein	0.2 M
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

5 Hetronuclear NOE

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Large Fragment of the SARS-CoV Fusion Protein	[U-13C; U-15N]	protein	0.2 M
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

6 3D 1H-15N NOESY

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Large Fragment of the SARS-CoV Fusion Protein	[U-13C; U-15N]	protein	0.2 M
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

7 3D 1H-15N TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	Large Fragment of the SARS-CoV Fusion Protein	[U-13C; U-15N]	protein	0.2 M
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

nullKeywords VIRAL PROTEIN

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Found 1 Document (3.201 seconds)

BMRB: 36080

Sample

Related entities 1. Spike protein S2, : 1 : 35 : 71 entities Detail

Experiments performed 7 experiments Detail

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