BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	83.5 % (920 of 1102)	82.0 % (468 of 571)	84.8 % (362 of 427)	86.5 % (90 of 104)
Backbone	91.4 % (530 of 580)	91.5 % (183 of 200)	90.8 % (258 of 284)	92.7 % (89 of 96)
Sidechain	77.3 % (473 of 612)	76.8 % (285 of 371)	80.3 % (187 of 233)	12.5 % (1 of 8)
Aromatic	47.8 % (43 of 90)	53.3 % (24 of 45)	42.2 % (19 of 45)
Methyl	92.4 % (85 of 92)	89.1 % (41 of 46)	95.7 % (44 of 46)

1. Small ubiquitin-related modifier

MHHHHHHMAD DAAQAGDNAE YIKIKVVGQD SNEVHFRVKY GTSMAKLKKS YADRTGVAVN SLRFLFDGRR INDDDTPKTL EMEDDDVIEV YQEQLGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 620 uM [U-13C; U-15N] SMO1, Small Ubiquitin like modifier in C. elegans, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.2514495
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.2514495
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.2514495
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.78 ppm	internal	indirect	0.2514495
¹H	water	protons	4.78 ppm	internal	direct	1.0
¹⁵N	water	protons	4.78 ppm	internal	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5XQM, Strand ID: A Detail

Release date

2017-08-27

Citation

Structural and functional analysis of SMO-1, the SUMO homolog in Caenorhabditis elegans
Surana, P., Gowda, C.M., Tripathi, V., Broday, L., Das, R.
PLoS One (2017), 12, e0186622-e0186622, PubMed 29045470 , DOI 10.1371/journal.pone.0186622 , Abstract

Related entities 1. Small ubiquitin-related modifier, : 1 : 2 : 169 entities Detail

Interaction partners 1. Small ubiquitin-related modifier, : 24 interactors Detail

More details for 24 interactors identified by 4 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker AvanceIII - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0, Details 620 uM [U-13C; U-15N] SMO1, Small Ubiquitin like modifier in C. elegans, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

2 3D HNCO

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

3 3D HNCA

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

4 3D HNCACB

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

5 3D CBCA(CO)NH

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

6 3D HN(CO)CA

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

7 3D C(CO)NH

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

8 3D H(CCO)NH

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

9 3D 1H-15N NOESY

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

10 3D 1H-13C NOESY aliphatic

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

11 3D 1H-13C NOESY aromatic

Instrument

Bruker AvanceIII - 800 MHz

Sample

#	Name	Isotope labeling	Type	Concentration
1	SMO1, Small Ubiquitin like modifier in C. elegans	[U-13C; U-15N]		620 uM
2	NaCl	natural abundance	salt	150 mM
3	sodium phosphate	natural abundance	buffer	25 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36096_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36096_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	999		92.8 (chain: 1, length: 97)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 97, Sequence coverage: 92.8%
- Total number of assignments
  - ¹H chemical shifts: 547
  - ¹³C chemical shifts: 364
  - ¹⁵N chemical shifts: 88
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	571	465	81.4
¹³C chemical shifts	427	364	85.2
¹⁵N chemical shifts	109	88	80.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	200	183	91.5
¹³C chemical shifts	194	176	90.7
¹⁵N chemical shifts	96	88	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	371	282	76.0
¹³C chemical shifts	233	188	80.7
¹⁵N chemical shifts	13	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	41	82.0
¹³C chemical shifts	50	44	88.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	45	23	51.1
¹³C chemical shifts	45	19	42.2

Keywords Caenorhabditis elegans, SIGNALING PROTEIN, Sumo homologue, solution structure

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Found 1 Document (0.994 seconds)

BMRB: 36096

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Small ubiquitin-related modifier, : 1 : 2 : 169 entities Detail

Interaction partners 1. Small ubiquitin-related modifier, : 24 interactors Detail

Experiments performed 11 experiments Detail

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Chemical shift validation 3 contents Detail