BMRB: 36103

Pholiota squarrosa lectin trimer

Authors

Yamasaki, K.

Assembly

lectin (PhoSL)

Entity

1. lectin (PhoSL) (polymer, Thiol state: all free), 40 monomers, 4436.923 × 3 Da Detail

APVPVTKLVC DGDTYKCTAY LDYGDGKWVA QWDTAVFHTT

Total weight

13310.769 Da

Max. entity weight

4436.923 Da

Source organism

Pholiota squarrosa

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.1 %, Completeness (bb): 92.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.1 % (212 of 223)	95.1 % (212 of 223)
Backbone	92.6 % (75 of 81)	92.6 % (75 of 81)
Sidechain	96.5 % (137 of 142)	96.5 % (137 of 142)
Aromatic	100.0 % (31 of 31)	100.0 % (31 of 31)
Methyl	95.8 % (23 of 24)	95.8 % (23 of 24)

1. entity 1

APVPVTKLVC DGDTYKCTAY LDYGDGKWVA QWDTAVFHTT

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 7.5, Details 0.3 mM Pholiota squarrosa lectin (PhoSL), 95% H2O/5% D2O

#	Name	Isotope labeling	Type	Concentration
1	Pholiota squarrosa lectin (PhoSL)	natural abundance	protein	0.3 mM
2	TRIS	natural abundance	buffer	20 mM
3	H2O	natural abundance	solvent	95 %
4	D2O	[U-2H]	solvent	5 %

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Release date

2017-08-20

Citation

The trimeric solution structure and fucose-binding mechanism of the core fucosylation-specific lectin PhoSL
Yamasaki, K., Yamasaki, T., Tateno, H.
Sci. Rep. (2018), 8, 7740-7740, PubMed 29773815 , DOI 10.1038/s41598-018-25630-2 , Abstract

Related entities 1. lectin (PhoSL), : 1 : 3 : 100 entities Detail

Experiments performed 3 experiments Detail