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BMRB: 36112

5Y70

NMR structure of the domain 5 of the E. coli ribosomal protein S1

Authors

Lu, Y., Lim, L.Z., Song, J.

Assembly

30S ribosomal protein S1

Entity

1. 30S ribosomal protein S1 (polymer, Thiol state: not present), 100 monomers, 12043.34 Da Detail

MATTYEEFAA KLDRLDAEFA KKMEEQNKRF FADKPDEATL SPEMKEHYEK FEKMIQEHTD KFNKKMREHS EHFKAKFAEL LEQQKNAQFP GKGSHHHHHH

Formula weight

12043.34 Da

Source organism

Trypanosoma brucei brucei

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chemcal Shifts

¹H: 178, ¹³C: 177, ¹⁵N: 89 assignments Detail

ID	Entity ID	Seq ID	Comp ID	Atom ID	Atom type	Val
1	1	1	MET	H	H	8.388
2	1	1	MET	CA	C	56.099
3	1	1	MET	CB	C	32.952
4	1	1	MET	N	N	120.800
5	1	2	ALA	H	H	8.247
6	1	2	ALA	HA	H	4.298
7	1	2	ALA	CA	C	53.515
8	1	2	ALA	CB	C	19.201
9	1	2	ALA	N	N	123.762
10	1	3	THR	H	H	8.160
11	1	3	THR	HA	H	4.310
12	1	3	THR	CA	C	63.116
13	1	3	THR	CB	C	69.786
14	1	3	THR	N	N	112.485
15	1	4	THR	H	H	8.187
16	1	4	THR	HA	H	4.205
17	1	4	THR	CA	C	64.308
18	1	4	THR	CB	C	69.526
19	1	4	THR	N	N	116.598
20	1	5	TYR	H	H	8.532
21	1	5	TYR	HA	H	4.192
22	1	5	TYR	CA	C	61.223
23	1	5	TYR	CB	C	38.118
24	1	5	TYR	N	N	120.698
25	1	6	GLU	H	H	8.368
26	1	6	GLU	HA	H	3.862
27	1	6	GLU	CA	C	59.816
28	1	6	GLU	CB	C	29.576
29	1	6	GLU	N	N	119.038
30	1	7	GLU	H	H	8.168
31	1	7	GLU	HA	H	4.081
32	1	7	GLU	CA	C	58.825
33	1	7	GLU	CB	C	29.610
34	1	7	GLU	N	N	119.409
35	1	8	PHE	H	H	8.123
36	1	8	PHE	HA	H	4.195
37	1	8	PHE	CA	C	60.932
38	1	8	PHE	CB	C	39.141
39	1	8	PHE	N	N	119.420
40	1	9	ALA	H	H	8.581
41	1	9	ALA	HA	H	3.765
42	1	9	ALA	CA	C	55.042
43	1	9	ALA	CB	C	17.789
44	1	9	ALA	N	N	121.244
45	1	10	ALA	H	H	7.763
46	1	10	ALA	HA	H	4.143
47	1	10	ALA	CA	C	54.219
48	1	10	ALA	CB	C	18.362
49	1	10	ALA	N	N	118.533
50	1	11	LYS	H	H	7.702
51	1	11	LYS	HA	H	4.105
52	1	11	LYS	CA	C	57.760
53	1	11	LYS	CB	C	32.427
54	1	11	LYS	N	N	117.147
55	1	12	LEU	H	H	7.756
56	1	12	LEU	HA	H	3.983
57	1	12	LEU	CA	C	56.957
58	1	12	LEU	CB	C	41.548
59	1	12	LEU	N	N	118.985
60	1	13	ASP	H	H	7.947
61	1	13	ASP	HA	H	4.380
62	1	13	ASP	CA	C	56.264
63	1	13	ASP	CB	C	41.244
64	1	13	ASP	N	N	118.081
65	1	14	ARG	H	H	7.715
66	1	14	ARG	HA	H	4.231
67	1	14	ARG	CA	C	56.625
68	1	14	ARG	CB	C	30.220
69	1	14	ARG	N	N	117.034
70	1	15	LEU	H	H	7.813
71	1	15	LEU	HA	H	4.124
72	1	15	LEU	CA	C	56.349
73	1	15	LEU	CB	C	42.134
74	1	15	LEU	N	N	120.720
75	1	16	ASP	H	H	8.167
76	1	16	ASP	HA	H	4.507
77	1	16	ASP	CA	C	54.882
78	1	16	ASP	CB	C	41.907
79	1	16	ASP	N	N	119.611
80	1	17	ALA	H	H	8.436
81	1	17	ALA	HA	H	4.166
82	1	17	ALA	CA	C	54.804
83	1	17	ALA	CB	C	18.758
84	1	17	ALA	N	N	123.622
85	1	18	GLU	H	H	8.364
86	1	18	GLU	HA	H	4.139
87	1	18	GLU	CA	C	58.431
88	1	18	GLU	CB	C	29.261
89	1	18	GLU	N	N	118.100
90	1	19	PHE	H	H	8.436
91	1	19	PHE	HA	H	4.202
92	1	19	PHE	CA	C	61.176
93	1	19	PHE	CB	C	39.226
94	1	19	PHE	N	N	120.959
95	1	20	ALA	H	H	8.339
96	1	20	ALA	HA	H	3.924
97	1	20	ALA	CA	C	55.170
98	1	20	ALA	CB	C	18.106
99	1	20	ALA	N	N	120.781
100	1	21	LYS	H	H	7.886
101	1	21	LYS	HA	H	4.064
102	1	21	LYS	CA	C	58.762
103	1	21	LYS	CB	C	32.221
104	1	21	LYS	N	N	117.975
105	1	22	LYS	H	H	7.945
106	1	22	LYS	HA	H	4.120
107	1	22	LYS	CA	C	58.456
108	1	22	LYS	CB	C	32.041
109	1	22	LYS	N	N	119.565
110	1	23	MET	H	H	8.139
111	1	23	MET	HA	H	4.161
112	1	23	MET	CA	C	57.539
113	1	23	MET	CB	C	32.321
114	1	23	MET	N	N	118.319
115	1	24	GLU	H	H	8.157
116	1	24	GLU	HA	H	4.065
117	1	24	GLU	CA	C	59.033
118	1	24	GLU	CB	C	29.509
119	1	24	GLU	N	N	120.578
120	1	25	GLU	H	H	8.114
121	1	25	GLU	HA	H	4.010
122	1	25	GLU	CA	C	58.833
123	1	25	GLU	CB	C	29.751
124	1	25	GLU	N	N	119.153
125	1	26	GLN	H	H	8.120
126	1	26	GLN	HA	H	4.148
127	1	26	GLN	CA	C	57.301
128	1	26	GLN	CB	C	28.441
129	1	26	GLN	N	N	117.723
130	1	27	ASN	H	H	8.274
131	1	27	ASN	HA	H	4.583
132	1	27	ASN	CA	C	55.390
133	1	27	ASN	CB	C	39.054
134	1	27	ASN	N	N	118.157
135	1	28	LYS	H	H	7.953
136	1	28	LYS	HA	H	4.124
137	1	28	LYS	CA	C	58.301
138	1	28	LYS	CB	C	32.535
139	1	28	LYS	N	N	118.711
140	1	29	ARG	H	H	7.908
141	1	29	ARG	HA	H	4.129
142	1	29	ARG	CA	C	57.379
143	1	29	ARG	CB	C	30.420
144	1	29	ARG	N	N	118.471
145	1	30	PHE	H	H	8.044
146	1	30	PHE	HA	H	4.335
147	1	30	PHE	CA	C	59.307
148	1	30	PHE	CB	C	39.746
149	1	30	PHE	N	N	118.350
150	1	31	PHE	H	H	7.964
151	1	31	PHE	HA	H	4.812
152	1	31	PHE	CA	C	57.692
153	1	31	PHE	CB	C	39.399
154	1	31	PHE	N	N	116.250
155	1	32	ALA	H	H	7.880
156	1	32	ALA	HA	H	4.274
157	1	32	ALA	CA	C	52.919
158	1	32	ALA	CB	C	19.661
159	1	32	ALA	N	N	122.951
160	1	33	ASP	H	H	8.251
161	1	33	ASP	HA	H	4.549
162	1	33	ASP	CA	C	54.487
163	1	33	ASP	CB	C	41.316
164	1	33	ASP	N	N	118.303
165	1	34	LYS	H	H	8.023
166	1	34	LYS	HA	H	4.578
167	1	34	LYS	CA	C	54.051
168	1	34	LYS	CB	C	32.738
169	1	34	LYS	N	N	120.459
170	1	36	ASP	H	H	8.413
171	1	36	ASP	HA	H	4.483
172	1	36	ASP	CA	C	54.538
173	1	36	ASP	CB	C	41.051
174	1	36	ASP	N	N	118.508
175	1	37	GLU	H	H	8.231
176	1	37	GLU	HA	H	4.219
177	1	37	GLU	CA	C	56.930
178	1	37	GLU	CB	C	30.414
179	1	37	GLU	N	N	120.244
180	1	38	ALA	H	H	8.211
181	1	38	ALA	HA	H	4.306
182	1	38	ALA	CA	C	52.725
183	1	38	ALA	CB	C	19.214
184	1	38	ALA	N	N	123.584
185	1	39	THR	H	H	8.059
186	1	39	THR	HA	H	4.233
187	1	39	THR	CA	C	62.475
188	1	39	THR	CB	C	69.880
189	1	39	THR	N	N	113.312
190	1	40	LEU	H	H	8.114
191	1	40	LEU	HA	H	4.464
192	1	40	LEU	CA	C	54.429
193	1	40	LEU	CB	C	43.158
194	1	40	LEU	N	N	122.371
195	1	41	SER	H	H	8.448
196	1	41	SER	HA	H	4.235
197	1	41	SER	CA	C	56.841
198	1	41	SER	CB	C	63.082
199	1	41	SER	N	N	119.193
200	1	43	GLU	H	H	8.853
201	1	43	GLU	HA	H	4.103
202	1	43	GLU	CA	C	59.467
203	1	43	GLU	CB	C	29.055
204	1	43	GLU	N	N	117.417
205	1	44	MET	H	H	7.919
206	1	44	MET	HA	H	4.412
207	1	44	MET	CA	C	57.510
208	1	44	MET	CB	C	32.473
209	1	44	MET	N	N	118.894
210	1	45	LYS	H	H	8.329
211	1	45	LYS	HA	H	3.960
212	1	45	LYS	CA	C	60.057
213	1	45	LYS	CB	C	32.352
214	1	45	LYS	N	N	120.079
215	1	46	GLU	H	H	7.995
216	1	46	GLU	HA	H	4.104
217	1	46	GLU	CA	C	59.245
218	1	46	GLU	CB	C	29.341
219	1	46	GLU	N	N	118.410
220	1	47	HIS	H	H	7.804
221	1	47	HIS	HA	H	4.483
222	1	47	HIS	CA	C	59.079
223	1	47	HIS	CB	C	30.471
224	1	47	HIS	N	N	117.568
225	1	48	TYR	H	H	8.499
226	1	48	TYR	HA	H	4.166
227	1	48	TYR	CA	C	61.996
228	1	48	TYR	CB	C	38.354
229	1	48	TYR	N	N	119.563
230	1	49	GLU	H	H	8.513
231	1	49	GLU	HA	H	3.976
232	1	49	GLU	CA	C	59.756
233	1	49	GLU	CB	C	29.387
234	1	49	GLU	N	N	118.964
235	1	50	LYS	H	H	7.921
236	1	50	LYS	HA	H	4.024
237	1	50	LYS	CA	C	59.370
238	1	50	LYS	CB	C	32.405
239	1	50	LYS	N	N	118.752
240	1	51	PHE	H	H	8.158
241	1	51	PHE	HA	H	4.316
242	1	51	PHE	CA	C	60.719
243	1	51	PHE	CB	C	39.117
244	1	51	PHE	N	N	120.508
245	1	52	GLU	H	H	8.649
246	1	52	GLU	HA	H	3.583
247	1	52	GLU	CA	C	60.163
248	1	52	GLU	CB	C	29.414
249	1	52	GLU	N	N	119.731
250	1	53	LYS	H	H	7.869
251	1	53	LYS	HA	H	4.023
252	1	53	LYS	CA	C	59.084
253	1	53	LYS	CB	C	32.214
254	1	53	LYS	N	N	117.814
255	1	54	MET	H	H	7.853
256	1	54	MET	HA	H	4.252
257	1	54	MET	CA	C	59.011
258	1	54	MET	CB	C	33.008
259	1	54	MET	N	N	118.908
260	1	55	ILE	H	H	8.085
261	1	55	ILE	HA	H	3.706
262	1	55	ILE	CA	C	64.110
263	1	55	ILE	CB	C	37.334
264	1	55	ILE	N	N	117.927
265	1	56	GLN	H	H	8.087
266	1	56	GLN	HA	H	4.027
267	1	56	GLN	CA	C	58.886
268	1	56	GLN	CB	C	28.525
269	1	56	GLN	N	N	119.951
270	1	57	GLU	H	H	8.081
271	1	57	GLU	HA	H	4.217
272	1	57	GLU	CA	C	58.434
273	1	57	GLU	CB	C	29.741
274	1	57	GLU	N	N	117.397
275	1	58	HIS	H	H	7.920
276	1	58	HIS	HA	H	4.683
277	1	58	HIS	CA	C	57.607
278	1	58	HIS	CB	C	31.092
279	1	58	HIS	N	N	115.943
280	1	59	THR	H	H	8.019
281	1	59	THR	HA	H	4.265
282	1	59	THR	CA	C	65.776
283	1	59	THR	CB	C	68.938
284	1	59	THR	N	N	114.152
285	1	60	ASP	H	H	8.509
286	1	60	ASP	HA	H	4.496
287	1	60	ASP	CA	C	56.963
288	1	60	ASP	CB	C	40.783
289	1	60	ASP	N	N	121.204
290	1	61	LYS	H	H	7.985
291	1	61	LYS	HA	H	4.104
292	1	61	LYS	CA	C	58.361
293	1	61	LYS	CB	C	32.400
294	1	61	LYS	N	N	118.570
295	1	62	PHE	H	H	8.143
296	1	62	PHE	HA	H	4.335
297	1	62	PHE	CA	C	59.935
298	1	62	PHE	CB	C	39.317
299	1	62	PHE	N	N	118.785
300	1	63	ASN	H	H	8.443
301	1	63	ASN	HA	H	4.358
302	1	63	ASN	CA	C	55.746
303	1	63	ASN	CB	C	38.814
304	1	63	ASN	N	N	118.722
305	1	64	LYS	H	H	8.204
306	1	64	LYS	HA	H	4.001
307	1	64	LYS	CA	C	59.475
308	1	64	LYS	CB	C	32.582
309	1	64	LYS	N	N	119.324
310	1	65	LYS	H	H	8.052
311	1	65	LYS	HA	H	4.216
312	1	65	LYS	CA	C	57.894
313	1	65	LYS	CB	C	32.164
314	1	65	LYS	N	N	118.710
315	1	66	MET	H	H	8.346
316	1	66	MET	HA	H	4.176
317	1	66	MET	CA	C	58.261
318	1	66	MET	CB	C	32.155
319	1	66	MET	N	N	118.027
320	1	67	ARG	H	H	8.071
321	1	67	ARG	HA	H	4.028
322	1	67	ARG	CA	C	59.076
323	1	67	ARG	CB	C	29.638
324	1	67	ARG	N	N	118.577
325	1	68	GLU	H	H	8.090
326	1	68	GLU	HA	H	4.057
327	1	68	GLU	CA	C	58.620
328	1	68	GLU	CB	C	29.761
329	1	68	GLU	N	N	118.790
330	1	69	HIS	H	H	7.988
331	1	69	HIS	HA	H	4.610
332	1	69	HIS	CA	C	57.494
333	1	69	HIS	CB	C	30.947
334	1	69	HIS	N	N	116.286
335	1	70	SER	H	H	8.199
336	1	70	SER	HA	H	4.092
337	1	70	SER	CA	C	58.611
338	1	70	SER	CB	C	63.088
339	1	70	SER	N	N	116.041
340	1	71	GLU	H	H	8.463
341	1	71	GLU	HA	H	4.273
342	1	71	GLU	CA	C	59.550
343	1	71	GLU	CB	C	29.136
344	1	71	GLU	N	N	120.952
345	1	72	HIS	H	H	8.044
346	1	72	HIS	HA	H	4.528
347	1	72	HIS	CA	C	58.555
348	1	72	HIS	CB	C	30.251
349	1	72	HIS	N	N	119.263
350	1	73	PHE	H	H	8.369
351	1	73	PHE	HA	H	4.196
352	1	73	PHE	CA	C	61.317
353	1	73	PHE	CB	C	38.974
354	1	73	PHE	N	N	118.328
355	1	74	LYS	H	H	8.543
356	1	74	LYS	HA	H	3.878
357	1	74	LYS	CA	C	60.914
358	1	74	LYS	CB	C	32.337
359	1	74	LYS	N	N	119.056
360	1	75	ALA	H	H	7.942
361	1	75	ALA	HA	H	4.202
362	1	75	ALA	CA	C	54.999
363	1	75	ALA	CB	C	18.106
364	1	75	ALA	N	N	120.578
365	1	76	LYS	H	H	8.043
366	1	76	LYS	HA	H	4.147
367	1	76	LYS	CA	C	57.416
368	1	76	LYS	CB	C	31.466
369	1	76	LYS	N	N	118.218
370	1	77	PHE	H	H	8.558
371	1	77	PHE	HA	H	4.169
372	1	77	PHE	CA	C	61.099
373	1	77	PHE	CB	C	39.023
374	1	77	PHE	N	N	119.589
375	1	78	ALA	H	H	8.193
376	1	78	ALA	HA	H	3.937
377	1	78	ALA	CA	C	55.502
378	1	78	ALA	CB	C	18.046
379	1	78	ALA	N	N	120.049
380	1	79	GLU	H	H	7.886
381	1	79	GLU	HA	H	4.056
382	1	79	GLU	CA	C	59.042
383	1	79	GLU	CB	C	29.893
384	1	79	GLU	N	N	117.933
385	1	80	LEU	H	H	7.978
386	1	80	LEU	HA	H	4.058
387	1	80	LEU	CA	C	57.666
388	1	80	LEU	CB	C	41.907
389	1	80	LEU	N	N	120.177
390	1	81	LEU	H	H	7.997
391	1	81	LEU	HA	H	3.946
392	1	81	LEU	CA	C	57.299
393	1	81	LEU	CB	C	41.732
394	1	81	LEU	N	N	118.294
395	1	82	GLU	H	H	7.898
396	1	82	GLU	HA	H	4.044
397	1	82	GLU	CA	C	58.549
398	1	82	GLU	CB	C	29.526
399	1	82	GLU	N	N	119.098
400	1	83	GLN	H	H	8.083
401	1	83	GLN	HA	H	4.129
402	1	83	GLN	CA	C	57.630
403	1	83	GLN	CB	C	28.731
404	1	83	GLN	N	N	118.203
405	1	84	GLN	H	H	8.138
406	1	84	GLN	HA	H	4.144
407	1	84	GLN	CA	C	56.911
408	1	84	GLN	CB	C	28.560
409	1	84	GLN	N	N	118.520
410	1	85	LYS	H	H	8.084
411	1	85	LYS	HA	H	4.118
412	1	85	LYS	CA	C	58.015
413	1	85	LYS	CB	C	32.568
414	1	85	LYS	N	N	120.172
415	1	86	ASN	H	H	8.135
416	1	86	ASN	HA	H	4.611
417	1	86	ASN	CA	C	53.706
418	1	86	ASN	CB	C	38.916
419	1	86	ASN	N	N	117.466
420	1	87	ALA	H	H	7.948
421	1	87	ALA	HA	H	4.219
422	1	87	ALA	CA	C	52.932
423	1	87	ALA	CB	C	19.155
424	1	87	ALA	N	N	123.063
425	1	88	GLN	H	H	8.187
426	1	88	GLN	HA	H	4.276
427	1	88	GLN	CA	C	55.738
428	1	88	GLN	CB	C	29.498
429	1	88	GLN	N	N	118.133
430	1	89	PHE	H	H	8.174
431	1	89	PHE	HA	H	4.861
432	1	89	PHE	CA	C	55.452
433	1	89	PHE	CB	C	39.203
434	1	89	PHE	N	N	121.116
435	1	91	GLY	H	H	8.190
436	1	91	GLY	HA2	H	3.901
437	1	91	GLY	HA3	H	3.901
438	1	91	GLY	CA	C	45.373
439	1	91	GLY	N	N	109.133
440	1	92	LYS	H	H	7.764
441	1	92	LYS	HA	H	4.147
442	1	92	LYS	CA	C	57.536
443	1	92	LYS	CB	C	33.689
444	1	92	LYS	N	N	125.689

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

Release date

2017-10-17

Citation

Kinetoplastid membrane protein-11 adopts a four-helix bundle fold in DPC micelle
Lim, L.Z., Ee, S., Fu, J., Tan, Y., He, C.Y., Song, J.
FEBS Lett. (2017), 591, 3793-3804, PubMed 29082514 , DOI 10.1002/1873-3468.12891 , Abstract

Related entities 1. 30S ribosomal protein S1, : 1 : 2 : 13 entities Detail

Experiments performed 4 experiments Detail

#	Name	Isotope labeling	Type	Concentration
1	KMP-11	[U-99% 13C; U-99% 15N]	protein	500 uM
2	DPC	natural abundance		150 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

#	Name	Isotope labeling	Type	Concentration
1	KMP-11	[U-99% 13C; U-99% 15N]	protein	500 uM
2	DPC	natural abundance		150 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

#	Name	Isotope labeling	Type	Concentration
1	KMP-11	[U-99% 13C; U-99% 15N]	protein	500 uM
2	DPC	natural abundance		150 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

#	Name	Isotope labeling	Type	Concentration
1	KMP-11	[U-99% 13C; U-99% 15N]	protein	500 uM
2	DPC	natural abundance		150 mM
3	H2O	natural abundance	solvent	90 %
4	D2O	[U-2H]	solvent	10 %

nullKeywords E. coli ribosomal protein, NMR solution structure, RIBOSOMAL PROTEIN, S1 RNA-binding domain

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Related entities 1. 30S ribosomal protein S1, : 1 : 2 : 13 entities Detail

Experiments performed 4 experiments Detail

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