BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	81.3 % (557 of 685)	88.7 % (321 of 362)	67.9 % (180 of 265)	96.6 % (56 of 58)
Backbone	75.0 % (252 of 336)	89.7 % (104 of 116)	58.8 % (100 of 170)	96.0 % (48 of 50)
Sidechain	88.3 % (354 of 401)	88.2 % (217 of 246)	87.8 % (129 of 147)	100.0 % (8 of 8)
Aromatic	63.2 % (48 of 76)	65.8 % (25 of 38)	58.3 % (21 of 36)	100.0 % (2 of 2)
Methyl	100.0 % (34 of 34)	100.0 % (17 of 17)	100.0 % (17 of 17)

1. entity 1

SFEIPDDVPL PAGWEMAKTS SGQRYFLNHI DQTTTWQDPR KAMGGGGNYQ GPPPPYPKH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details 1.0 mM [U-100% 13C; U-100% 15N] WW domain with PPxY motif, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	WW domain with PPxY motif	[U-100% 13C; U-100% 15N]	protein	1.0 (±0.1) mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details 1 mM WW domain with PPxY motif, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	WW domain with PPxY motif	natural abundance	protein	1 (±0.1) mM
5	D2O	[U-2H]	solvent	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.735 ppm	na	indirect	0.2514495
¹H	water	protons	4.735 ppm	internal	direct	1.0
¹⁵N	water	protons	4.735 ppm	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.735 ppm	na	indirect	0.2514495
¹H	water	protons	4.735 ppm	internal	direct	1.0
¹⁵N	water	protons	4.735 ppm	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.735 ppm	na	indirect	0.2514495
¹H	water	protons	4.735 ppm	internal	direct	1.0
¹⁵N	water	protons	4.735 ppm	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5YDX, Strand ID: A Detail

Release date

2017-10-17

Citation

Biophysical studies and NMR structure of YAP2 WW domain - LATS1 PPxY motif complexes reveal the basis of their interaction
Verma, A., Jing-Song, F., Finch-Edmondson, M.L., Velazquez-Campoy, A., Balasegaran, S., Sudol, M., Sivaraman, J.
Oncotarget (2018), 9, 8068-8080, PubMed 29487715 , DOI 10.18632/oncotarget.23909 , Abstract

Related entities 1. WW domain with PPxY motif, : 1 : 94 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr36114_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr36114_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chemical_shifts_1	Warning	582		96.6 (chain: 1, length: 59)
1	Chemical shift references	chem_shift_reference_1	OK	3		No information

Assigned chemical shifts

Saveframe: assigned_chemical_shifts_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 59, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 356
  - ¹³C chemical shifts: 170
  - ¹⁵N chemical shifts: 56
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	362	318	87.8
¹³C chemical shifts	265	170	64.2
¹⁵N chemical shifts	60	56	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	116	102	87.9
¹³C chemical shifts	118	41	34.7
¹⁵N chemical shifts	50	47	94.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	246	216	87.8
¹³C chemical shifts	147	129	87.8
¹⁵N chemical shifts	10	9	90.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	19	19	100.0
¹³C chemical shifts	19	19	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	38	25	65.8
¹³C chemical shifts	36	21	58.3
¹⁵N chemical shifts	2	2	100.0

Keywords Hippo pathway, LATs, PPxY motif, SIGNALING PROTEIN, WW domain, YAP

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Found 1 Document (0.867 seconds)

BMRB: 36114

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. WW domain with PPxY motif, : 1 : 94 entities Detail

Experiments performed 5 experiments Detail

Instrument

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Chemical shift validation 3 contents Detail