BMRB: 36115

NMR structure of YAP1-2 WW2 domain with LATS1 PPxY motif complex

Authors

Fan, J.S., Sivaraman, J.

Assembly

WW2 domain and PPxY motif complex

Entity

1. WW2 domain and PPxY motif complex (polymer, Thiol state: not present), 54 monomers, 5991.531 Da Detail

SGPLPDGWEQ AMTQDGEIYY INHKNKTTSW LDPRLDPRGG GGNRQPPPPY PLTA

Formula weight

5991.531 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.6 %, Completeness: 77.5 %, Completeness (bb): 84.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	77.5 % (297 of 383)	75.2 % (248 of 330)	92.5 % (49 of 53)
Backbone	84.8 % (128 of 151)	82.1 % (87 of 106)	91.1 % (41 of 45)
Sidechain	72.8 % (169 of 232)	71.9 % (161 of 224)	100.0 % (8 of 8)
Aromatic	78.6 % (22 of 28)	76.9 % (20 of 26)	100.0 % (2 of 2)
Methyl	94.4 % (17 of 18)	94.4 % (17 of 18)

1. entity 1

SGPLPDGWEQ AMTQDGEIYY INHKNKTTSW LDPRLDPRGG GGNRQPPPPY PLTA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details 1.0 mM [U-100% 15N] WW2 domain and PPxY motif complex, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	WW2 domain and PPxY motif complex	[U-100% 15N]	protein	1.0 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 (±0.01) atm, Temperature 298 (±0.1) K, pH 6.0 (±0.1), Details 1.00 mM WW2 domain and PPxY motif complex, 100% D2O

#	Name	Isotope labeling	Type	Concentration
4	WW2 domain and PPxY motif complex	natural abundance	protein	1.00 mM
5	D2O	[U-2H]	solvent	100 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 5YDY, Strand ID: A Detail

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Release date

2017-10-17

Citation

Biophysical studies and NMR structure of YAP2 WW domain - LATS1 PPxY motif complexes reveal the basis of their interaction
Verma, A., Jing-Song, F., Finch-Edmondson, M.L., Velazquez-Campoy, A., Balasegaran, S., Sudol, M., Sivaraman, J.
Oncotarget (2018), 9, 8068-8080, PubMed 29487715 , DOI 10.18632/oncotarget.23909 , Abstract

Related entities 1. WW2 domain and PPxY motif complex, : 1 : 81 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail