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BMRB: 36117

5YFG

SOLUTION STRUCTURE OF HUMAN MOG1

Authors

Hu, Q., Liu, Y., Bao, X., Liu, H.

Assembly

Ran guanine nucleotide release factor

Entity

1. Ran guanine nucleotide release factor (polymer, Thiol state: all free), 194 monomers, 21498.92 Da Detail

MEPTRDCPLF GGAFSAILPM GAIDVSDLRP VPDNQEVFCH PVTDQSLIVE LLELQAHVRG EAAARYHFED VGGVQGARAV HVESVQPLSL ENLALRGRCQ EAWVLSGKQQ IAKENQQVAK DVTLHQALLR LPQYQTDLLL TFNQPPPDNR SSLGPENLSP APWSLGDFEQ LVTSLTLHDP NIFGPQVEHH HHHH

Formula weight

21498.92 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING, DISTANCE GEOMETRYtorsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.3 %, Completeness: 68.5 %, Completeness (bb): 86.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	68.5 % (1524 of 2226)	66.1 % (764 of 1155)	68.8 % (597 of 868)	80.3 % (163 of 203)
Backbone	86.7 % (981 of 1132)	87.0 % (334 of 384)	84.9 % (484 of 570)	91.6 % (163 of 178)
Sidechain	54.4 % (694 of 1276)	55.8 % (430 of 771)	55.0 % (264 of 480)	0.0 % (0 of 25)
Aromatic	9.5 % (15 of 158)	13.9 % (11 of 79)	5.2 % (4 of 77)	0.0 % (0 of 2)
Methyl	73.7 % (168 of 228)	78.9 % (90 of 114)	68.4 % (78 of 114)

1. Ran guanine nucleotide release factor

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 0.9 mM [U-99% 15N] Mog1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Mog1	[U-99% 15N]	protein	0.9 (±0.1) mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 0.9 mM [U-13C] Mog1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	Mog1	[U-13C]	protein	0.9 (±0.1) mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 0.9 mM [U-99% 13C; U-99% 15N] Mog1, 100% D2O

#	Name	Isotope labeling	Type	Concentration
7	Mog1	[U-99% 13C; U-99% 15N]	protein	0.9 (±0.1) mM
8	D2O	[U-2H]	solvent	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.48 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5YFG, Strand ID: A Detail

Release date

2017-10-19

Citation

Mitosis-specific acetylation tunes Ran effector binding for chromosome segregation
Bao, X., Liu, H., Liu, X., Ruan, K., Zhang, Y., Zhang, Z., Hu, Q., Liu, Y., Akram, S., Zhang, J., Gong, Q., Wang, W., Yuan, X., Dou, Z., Tian, R., Yao, X., Wu, J., Shi, Y.
J. Mol. Cell Biol. (2018), 10, 18-32, PubMed 29040603 , DOI 10.1093/jmcb/mjx045 , Abstract

Related entities 1. Ran guanine nucleotide release factor, : 1 : 2 : 20 entities Detail

Interaction partners 1. Ran guanine nucleotide release factor, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 0.9 mM [U-99% 15N] Mog1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	Mog1	[U-99% 15N]	protein	0.9 (±0.1) mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

2 2D 1H-13C HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 0.9 mM [U-13C] Mog1, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
4	Mog1	[U-13C]	protein	0.9 (±0.1) mM
5	H2O	natural abundance	solvent	90 %
6	D2O	[U-2H]	solvent	10 %

3 2D 1H-1H TOCSY