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BMRB: 36143

5YZ9

zinc finger domain of METTL3-METTL14 N6-methyladenosine methyltransferase

Authors

Dong, X., Tang, C., Gong, Z., Yin, P., Huang, J.B.

Assembly

N6-adenosine-methyltransferase catalytic subunit (E.C.2.1.1.62)

Entity

1. N6-adenosine-methyltransferase catalytic subunit (E.C.2.1.1.62), entity 1 (polymer, Thiol state: all other bound), 102 monomers, 11733.03 Da Detail

AHMSIVEKFR SRGRAQVQEF CDYGTKEECM KASDADRPCR KLHFRRIINK HTDESLGDCS FLNTCFHMDT CKYVHYEIDA SMDSEAPGSK DHTPSQELAL TQ

2. ZINC ION (non-polymer), 65.409 × 2 Da

Total weight

11863.849 Da

Max. entity weight

11733.03 Da

Entity Connection

metal coordination 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS21:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS29:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS39:SG	2:ZN1:ZN
4	metal coordination	sing	1:HIS43:NE2	2:ZN1:ZN
5	metal coordination	sing	1:CYS59:SG	2:ZN1:ZN
6	metal coordination	sing	1:CYS65:SG	2:ZN1:ZN
7	metal coordination	sing	1:CYS71:SG	2:ZN1:ZN
8	metal coordination	sing	1:HIS75:NE2	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.1 %, Completeness: 76.6 %, Completeness (bb): 94.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.6 % (893 of 1166)	71.6 % (437 of 610)	80.0 % (361 of 451)	90.5 % (95 of 105)
Backbone	94.4 % (572 of 606)	94.6 % (194 of 205)	93.7 % (283 of 302)	96.0 % (95 of 99)
Sidechain	62.8 % (413 of 658)	60.0 % (243 of 405)	68.8 % (170 of 247)	0.0 % (0 of 6)
Aromatic	26.5 % (26 of 98)	40.8 % (20 of 49)	12.2 % (6 of 49)
Methyl	85.1 % (63 of 74)	78.4 % (29 of 37)	91.9 % (34 of 37)

1. entity 1

AHMSIVEKFR SRGRAQVQEF CDYGTKEECM KASDADRPCR KLHFRRIINK HTDESLGDCS FLNTCFHMDT CKYVHYEIDA SMDSEAPGSK DHTPSQELAL TQ

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 6.8, Details 20 mM PBS, 100 mM sodium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ZFD	[U-13C; U-15N]	protein	0.8 mM
2	PBS	natural abundance	buffer	20 mM
3	sodium chloride	natural abundance	salt	100 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	na	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	na	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 2 Detail

Release date

2018-05-22

Citation

Solution structure of the RNA recognition domain of METTL3-METTL14 N⁶-methyladenosine methyltransferase
Huang, J., Dong, X., Gong, Z., Qin, L.Y., Yang, S., Zhu, Y.L., Wang, X., Zhang, D., Zou, T., Yin, P., Tang, C.
Protein. Cell (2018), 10, 272-284, PubMed 29542011 , DOI 10.1007/s13238-018-0518-7 , Abstract

Related entities 1. N6-adenosine-methyltransferase catalytic subunit (E.C.2.1.1.62), entity 1, : 1 : 3 : 5 entities Detail

Interaction partners 1. N6-adenosine-methyltransferase catalytic subunit (E.C.2.1.1.62), entity 1, : 6 interactors Detail

More details for 6 interactors identified by 3 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC