BMRB: 36157

Nukacin ISK-1 in active state

Authors

Kohda, D., Fujinami, D.

Assembly

Lantibiotic nukacin

Entity

1. Lantibiotic nukacin (polymer, Thiol state: all other bound), 27 monomers, 2965.493 Da Detail

KKKSGVIPXV XHDCHMNXFQ FVFXCCS

Formula weight

2965.493 Da

Entity Connection

thioether 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	thioether	sing	1:DBB9:CB	1:CYS14:SG
2	thioether	sing	1:DAL11:CB	1:CYS25:SG
3	thioether	sing	1:DAL18:CB	1:CYS26:SG

Source organism

Staphylococcus warneri

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.2 %, Completeness: 96.6 %, Completeness (bb): 97.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	96.6 % (141 of 146)	96.6 % (141 of 146)
Backbone	97.8 % (45 of 46)	97.8 % (45 of 46)
Sidechain	96.0 % (96 of 100)	96.0 % (96 of 100)
Aromatic	100.0 % (19 of 19)	100.0 % (19 of 19)
Methyl	100.0 % (8 of 8)	100.0 % (8 of 8)

1. Lantibiotic nukacin

KKKSGVIPXV XHDCHMNXFQ FVFXCCS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 328 K, pH 3.5, Details 13 mM nukacin ISK-1, 34 mM [U-2H] dodecylphosphocholine, 10 mM glycine, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	dodecylphosphocholine	[U-2H]		34 mM
2	glycine	natural abundance		10 mM
3	nukacin ISK-1	natural abundance	protein	13 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	[U-2H]	solvent	10 %

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Protein Blocks Logo

Calculated from 20 models in PDB: 5Z5Q, Strand ID: A Detail

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Release date

2019-01-26

Citation

The lantibiotic nukacin ISK-1 exists in an equilibrium between active and inactive lipid-II binding states
Fujinami, D., Mahin, A.A., Elsayed, K.M., Islam, M.R., Nagao, J.I., Roy, U., Momin, S., Zendo, T., Kohda, D., Sonomoto, K.
Commun. Biol. (2018), 1, 150-150, PubMed 30272026 , DOI 10.1038/s42003-018-0150-3 , Abstract

Related entities 1. Lantibiotic nukacin, : 1 : 1 : 1 : 7 entities Detail

Experiments performed 2 experiments Detail

Chemical shift validation 3 contents Detail