Solution structure for the unique dimeric 4:2 complex of a platinum(II)-based tripod bound to a hybrid-1 human telomeric G-quadruplex
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 98.3 % (236 of 240) | 98.3 % (236 of 240) |
Suger, PO4 | 100.0 % (182 of 182) | 100.0 % (182 of 182) |
Nucleobase | 93.1 % (54 of 58) | 93.1 % (54 of 58) |
Aromatic | 92.3 % (48 of 52) | 92.3 % (48 of 52) |
Methyl | 100.0 % (6 of 6) | 100.0 % (6 of 6) |
1. G-quadruplex DNA (26-MER)
AAAGGGTTAG GGTTAGGGTT AGGGAASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM 15N Thymine G-quadruplex DNA (26-MER), 3 mM Pt-tripod, 25 mM potassium phosphate, 70 mM potassium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | G-quadruplex DNA (26-MER) | [U-15N]-Thy | protein | 1 mM |
2 | Pt-tripod | natural abundance | 3 mM | |
3 | potassium chloride | natural abundance | salt | 70 mM |
4 | potassium phosphate | natural abundance | buffer | 25 mM |
5 | H2O | natural abundance | solvent | 90 % |
6 | D2O | [U-2H] | solvent | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 1 mM G-quadruplex DNA (26-MER), 3 mM Pt-tripod, 25 mM potassium phosphate, 70 mM potassium chloride, 90% H2O/10% D2O
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | G-quadruplex DNA (26-MER) | natural abundance | protein | 1 mM |
8 | Pt-tripod | natural abundance | 3 mM | |
9 | potassium chloride | natural abundance | salt | 70 mM |
10 | potassium phosphate | natural abundance | buffer | 25 mM |
11 | H2O | natural abundance | solvent | 90 % |
12 | D2O | [U-2H] | solvent | 10 % |