BMRB: 36168

Solution structure of G-quadruplex formed in vegfr-2 proximal promoter sequence

Authors

Liu, Y., Lan, W.X.

Assembly

DNA (5'-D(*GP*GP*GP*TP*AP*CP*CP*CP*GP*GP*GP*TP*GP*AP*GP*GP*TP*GP*CP*GP*GP*GP*GP*T)-3')

Entity

1. DNA (5'-D(*GP*GP*GP*TP*AP*CP*CP*CP*GP*GP*GP*TP*GP*AP*GP*GP*TP*GP*CP*GP*GP*GP*GP*T)-3') (polymer, Thiol state: not present), 24 monomers, 7626.807 Da Detail

GGGTACCCGG GTGAGGTGCG GGGT

Formula weight

7626.807 Da

Source organism

synthetic construct

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.9 %, Completeness (bb): 93.8 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H	31P
All	88.9 % (224 of 252)	87.7 % (200 of 228)	100.0 % (24 of 24)
Suger, PO4	93.8 % (180 of 192)	92.9 % (156 of 168)	100.0 % (24 of 24)
Nucleobase	73.3 % (44 of 60)	73.3 % (44 of 60)
Aromatic	83.3 % (40 of 48)	83.3 % (40 of 48)
Methyl	100.0 % (4 of 4)	100.0 % (4 of 4)

1. entity 1

GGGTACCCGG GTGAGGTGCG GGGT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 1 mM DNA (5'-D(*GP*GP*GP*TP*AP*CP*CP*CP*GP*GP*GP*TP*GP*AP*GP*GP*TP*GP*CP*GP*GP*GP*GP*T)-3'), 20 mM potassium phosphate, 80 mM potassium chloride, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	DNA (5'-D(*GP*GP*GP*TP*AP*CP*CP*CP*GP*GP*GP*TP*GP*AP*GP*GP*TP*GP*CP*GP*GP*GP*GP*T)-3')	natural abundance	DNA	1 mM
2	potassium chloride	natural abundance	salt	80 mM
3	potassium phosphate	natural abundance	buffer	20 mM
4	H2O	natural abundance	solvent	90 %
5	D2O	[U-2H]	solvent	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8, Details 1.0 mM DNA (5'-D(*GP*GP*GP*TP*AP*CP*CP*CP*GP*GP*GP*TP*GP*AP*GP*GP*TP*GP*CP*GP*GP*GP*GP*T)-3'), 80 mM potassium chloride, 20 mM potassium phosphate, 100% D2O

#	Name	Isotope labeling	Type	Concentration
6	DNA (5'-D(*GP*GP*GP*TP*AP*CP*CP*CP*GP*GP*GP*TP*GP*AP*GP*GP*TP*GP*CP*GP*GP*GP*GP*T)-3')	natural abundance	DNA	1.0 mM
7	potassium chloride	natural abundance	salt	80 mM
8	potassium phosphate	natural abundance	buffer	20 mM
9	D2O	[U-2H]	solvent	100 %

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Release date

2018-04-16

Citation

A putative G-quadruplex structure in the proximal promoter of VEGFR-2 has implications for drug design to inhibit tumor angiogenesis
Liu, Y., Lan, W., Wang, C., Cao, C.
J. Biol. Chem. (2018), 293, 8947-8955, PubMed 29666187 , DOI 10.1074/jbc.RA118.002666 , Abstract

Experiments performed 5 experiments Detail