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BMRB: 36175

5ZMB

A-ubiquitin like protein from the trypanosoma brucei

Authors

Mi, J., Tu, X.M.

Assembly

ubiquintin-like protein

Entity

1. ubiquintin-like protein (polymer, Thiol state: all free), 81 monomers, 9129.364 Da Detail

MSETIPVSVQ CCEGRFELSV DSNHTLRDVL RQFKREVAAL DPINLEEYVV NHEGKLLLDD SVTLQTVGVK KDSVFVLVRK A

Formula weight

9129.364 Da

Source organism

Trypanosoma brucei brucei TREU927

Exptl. method

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 79.9 %, Completeness (bb): 82.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.9 % (752 of 941)	86.8 % (422 of 486)	67.3 % (249 of 370)	95.3 % (81 of 85)
Backbone	82.0 % (395 of 482)	96.3 % (157 of 163)	67.1 % (161 of 240)	97.5 % (77 of 79)
Sidechain	80.6 % (433 of 537)	82.0 % (265 of 323)	78.8 % (164 of 208)	66.7 % (4 of 6)
Aromatic	28.3 % (13 of 46)	56.5 % (13 of 23)	0.0 % (0 of 23)
Methyl	82.5 % (94 of 114)	86.0 % (49 of 57)	78.9 % (45 of 57)

1. ubiquintin-like protein

MSETIPVSVQ CCEGRFELSV DSNHTLRDVL RQFKREVAAL DPINLEEYVV NHEGKLLLDD SVTLQTVGVK KDSVFVLVRK A

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.5 mM [U-99% 13C; U-99% 15N] ubiquitin-like protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like protein	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

LACS Plot; CA

Referencing offset: 0.98 ppm, Outliers: 4 Detail

LACS Plot; CB

Referencing offset: 0.98 ppm, Outliers: 4 Detail

LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 5ZMB, Strand ID: A Detail

Release date

2018-09-30

Citation

Solution structure of a ubiquitin-like protein from Trypanosoma brucei
Mi, J., Zhang, J., Liao, S., Tu, X.
Protein Sci. (2018), 27, 1831-1836, PubMed 30058168 , DOI 10.1002/pro.3492 , Abstract

Related entities 1. ubiquintin-like protein, : 1 : 9 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.5 mM [U-99% 13C; U-99% 15N] ubiquitin-like protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like protein	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

2 3D CBCA(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.5 mM [U-99% 13C; U-99% 15N] ubiquitin-like protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like protein	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

3 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.5 mM [U-99% 13C; U-99% 15N] ubiquitin-like protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like protein	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

4 3D C(CO)NH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.5 mM [U-99% 13C; U-99% 15N] ubiquitin-like protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like protein	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

5 3D 1H-15N NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.5 mM [U-99% 13C; U-99% 15N] ubiquitin-like protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like protein	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

6 3D 1H-13C NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5, Details 0.5 mM [U-99% 13C; U-99% 15N] ubiquitin-like protein, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ubiquitin-like protein	[U-99% 13C; U-99% 15N]	protein	0.5 mM
2	H2O	natural abundance	solvent	90 %
3	D2O	[U-2H]	solvent	10 %

nullKeywords PROTEIN BINDING, STRUCTURE FROM CYANA 3.0